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Determination of atorvastatin in human plasma by magnetic solid-phase extraction combined with HPLC and application to a pharmacokinetic study

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dc.contributor.authorTekkeli, Serife Evrim Kepekci
dc.contributor.authorDurmus, ZEHRA
dc.contributor.authorOnal, Armagan
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2019-10-05T15:16:36Z
dc.date.available2019-10-05T15:16:36Z
dc.date.issued2016-01-01
dc.description.abstractA simple and sensitive analytical procedure by solid-phase extraction method combined with high-performance liquid chromatography and using of graphene-magnetite nanomaterials as sorbent has been developed for the determination of atorvastatin in human plasma. A magnetic solid-phase extraction method as a simple, fast, and efficient extraction technique has been used for sample preparation. A solid nanocomposite material, graphene nanosheets decorated with magnetite nanoparticles, was used as a magnetic adsorbent and the adsorption process was optimized in this study. RP C18 column was used with mobile phase composed of acetonitrile-10mM orthophosphoric acid by isocratic elution with the flow rate of 1mLmin(-1). Fluorimetric detection was used by the excitation wavelength at 282nm and the emission wavelength at 400nm. It was found that the calibration curve was linear in the 30-150ngmL(-1). Limit of detection and limit of quantitation values were found to be 10 and 30ngmL(-1), respectively. The intra-day and inter-day relative standard deviation values were less than 5.27%. It has been concluded that the new developed method provides fast, simple, cost reduced, and sensitive assay for atorvastatin determination in human plasma. This method is also applied to a pharmacokinetic study.
dc.description.sponsorshipBezmiâlem Vakıf Üniversitesi
dc.identifier
dc.identifier.citationTekkeli S. E. K. , Durmus Z., Onal A., -Determination of atorvastatin in human plasma by magnetic solid-phase extraction combined with HPLC and application to a pharmacokinetic study-, JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.39, ss.852-860, 2016
dc.identifier.urihttps://hdl.handle.net/20.500.12645/5877
dc.language.isoen
dc.titleDetermination of atorvastatin in human plasma by magnetic solid-phase extraction combined with HPLC and application to a pharmacokinetic study
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameSCANDINAVIAN JOURNAL OF UROLOGY
local.avesis.id919e1fa2-fb26-4627-afff-88ed14a2fef9
local.avesis.response5747
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery837d5047-762e-43db-b252-9f3d1399378f
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