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Two-dimensional liquid chromatographic analysis of ramelteon in human serum

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dc.contributor.authorTEKKELİ, ŞERİFE EVRİM
dc.contributor.authorSagirli, Olcay
dc.contributor.authorOnal, Armagan
dc.contributor.authorToker, Sidika Erturk
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2020-01-16T22:51:27Z
dc.date.available2020-01-16T22:51:27Z
dc.date.issued2019-12-01T00:00:00Z
dc.description.abstractIn this study, a selective and sensitive new 2D-LC method was developed for the determination of ramelteon in human serum. Separations were performed using the following systems: the first dimension consisted of a C18 (50 mm x 2.1 mm) pre-separation column and the mobile phase of 45% acetonitrile: 55% 10 mM ortho-phosphoric acid solution mixture and the second dimension consisted of a pentafluorophenyl propyl (150 mm x 4.6 mm) separating column and the mobile phase of 40% methanol: 60% 2.25 mM ortho-phosphoric acid solution mixture. Two systems were connected with a 6 port valve including 200 mu L sample loop and separations were performed continuously through injections. Detection wavelength has been selected as 203 nm for both systems. Extraction of drug substance from serum samples was performed using protein precipitation method with methanol and the calibration curve was linear over a concentration range of 1-50 ng/mL. The accuracy and precision of the method were determined as 1.49-4.24% and 1.75-3.63% ranges for RSD and RME, respectively. The absolute recovery of the drug was found as 80.28%. The developed method was successfully applied for the determination of ramelteon in serum samples collected from a volunteer who had received 8 mg ramelteon orally. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.description.sponsorshipİstanbul Üniversitesi
dc.identifier.citationSagirli O., Onal A., Toker S. E. , TEKKELİ Ş. E. , -Two-dimensional liquid chromatographic analysis of ramelteon in human serum-, ARABIAN JOURNAL OF CHEMISTRY, cilt.12, ss.2817-2822, 2019
dc.identifier.doi10.1016/j.arabjc.2015.06.021
dc.identifier.scopus84932160256
dc.identifier.urihttps://openaccess.bezmialem.edu.tr/handle/20.500.12645/13356
dc.identifier.wosWOS:000504900300109
dc.language.isoen
dc.titleTwo-dimensional liquid chromatographic analysis of ramelteon in human serum
dc.typeArticle
dspace.entity.typePublication
local.avesis.id35b46f6f-b0ea-4e7d-9a93-1a0fc768847b
local.publication.isinternational1
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery837d5047-762e-43db-b252-9f3d1399378f
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