Publication:
Identification of suitable reference genes for RT-qPCR studies in human parathyroid tissue glandular cells.

dc.contributor.authorGoncu B. S.
dc.contributor.institutionauthorGÖNCÜ, BEYZA SERVET
dc.date.accessioned2024-04-02T21:50:17Z
dc.date.available2024-04-02T21:50:17Z
dc.date.issued2024-06-20
dc.description.abstractIdentifying a proper reference gene allows us to understand fundamental changes in many biological processes. Normalization during gene expression analyses is essential for every tissue/cell type, including parathyroid tissue glandular cells. The quantitative method of gene expression analyses via qRT-PCR provides an accurate examination of every target gene. There are limited reports to present commonly used reference genes in human parathyroid tissues rather than for glandular cell types. This study aims to determine and compare the most stable to least stable genes for parathyroid tissue cells. 43 human parathyroid tissue and glandular cells obtained from primary and secondary hyperparathyroidism patients were isolated enzymatically by removing extracellular matrix components. After extraction of the total RNA, cDNA synthesis was performed, then qRT-PCR evaluated 14 candidate reference genes. Stability was determined by RefFinder software (Delta ct, BestKeeper, Genorm, and NormFinder algorithms), and the outcome was evaluated for five groups. Even if assessed with different groups, the most stable genes were RPLP0 and GAPDH, while the CLTC and RNA 18S were the least stable. We confirmed the comprehensive ranking of the most stable three genes alone with the NormFinder algorithm to understand intergroup variation and found that RPLP0>GAPDH>PGK1. Lastly, relative target gene (GCM2) expression comparisons revealed similar expression patterns for the most stable reference genes. The most stable reference gene is recommended for the stages where stability is evaluated using the results of four different approaches using RefFinder. We aspire for this study to assist future research to conduct thorough assessments of appropriate reference genes before engaging in gene expression analyses for parathyroid tissue
dc.identifier.citationGoncu B. S., "Identification of suitable reference genes for RT-qPCR studies in human parathyroid tissue glandular cells.", Gene, cilt.912, ss.148380, 2024
dc.identifier.doi10.1016/j.gene.2024.148380
dc.identifier.endpage148380
dc.identifier.issn0378-1119
dc.identifier.pubmed38490511
dc.identifier.scopus85188122121
dc.identifier.startpage148380
dc.identifier.urihttps://hdl.handle.net/20.500.12645/39190
dc.identifier.volume912
dc.relation.ispartofGene
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectNatural Sciences
dc.subjectTemel Bilimler (SCI)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectDoğa Bilimleri Genel
dc.subjectÇOK DİSİPLİNLİ BİLİMLER
dc.subjectNatural Sciences (SCI)
dc.subjectLife Sciences (LIFE)
dc.subjectNATURAL SCIENCES, GENERAL
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectMULTIDISCIPLINARY SCIENCES
dc.subjectMoleküler Biyoloji
dc.subjectMultidisipliner
dc.subjectMolecular Biology
dc.subjectMultidisciplinary
dc.titleIdentification of suitable reference genes for RT-qPCR studies in human parathyroid tissue glandular cells.
dc.typearticle
dspace.entity.typePublication
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