Publication: A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection
dc.contributor.author | Onal, Cem | |
dc.contributor.author | ASRAM SAĞIROĞLU, ALİ | |
dc.contributor.institutionauthor | TEKKELİ, ŞERİFE EVRİM | |
dc.contributor.institutionauthor | ASRAM SAĞIROĞLU, ALİ | |
dc.date.accessioned | 2020-10-22T16:01:27Z | |
dc.date.available | 2020-10-22T16:01:27Z | |
dc.date.issued | 2020-09-01T00:00:00Z | |
dc.description.abstract | To determine deferasirox in human breast milk a novel, sensitive high-performance liquid chromatographic method with fluorescence detection has been developed. Precolumn derivatization process is applied to achieve fluorescent analyte using 4-bromomethyl-7-methoxy coumarin (BrMmC) as a reagent and dibenzo-18-crown-6-ether as catalyst. The method depends on reverse phase chromatography. C(18)column has been used with 150 x 4.6 mm, 5 mu m particle size and the mobile phase consisting of acetonitrile:phosphate buffer (20 mM, pH 3) (70:30,v/v) with a flow rate of 1.0 mL/min. Fluorescence detection at 390 nm is provided with an excitation at 320 nm The linear range is 0.01-1.0 mu g/mL. The mean recovery has been calculated as 100.7% with a relative standard deviation (RSD) value less than 2%. The developed method was successfully applied for the determination of deferasirox in human breast milk samples. | |
dc.identifier.citation | Onal C., TEKKELİ Ş. E. , ASRAM SAĞIROĞLU A., -A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection-, CHROMATOGRAPHIA, 2020 | |
dc.identifier.doi | 10.1007/s10337-020-03953-5 | |
dc.identifier.scopus | 85090498154 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12645/23863 | |
dc.identifier.wos | WOS:000568232100001 | |
dc.title | A Liquid Chromatographic Analysis of Deferasirox in Human Breast Milk with Fluorimetric Detection | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.avesis.id | 65917669-06d7-4916-b99e-0910e03ae1aa | |
local.indexed.at | WOS | |
local.indexed.at | Scopus | |
local.publication.isinternational | 1 | |
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relation.isAuthorOfPublication | 8d4d976d-a790-465b-8db9-1707530c0551 | |
relation.isAuthorOfPublication.latestForDiscovery | 837d5047-762e-43db-b252-9f3d1399378f |