Publication:
Iron affects localization of Ght5 in fission yeast.

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dc.contributor.authorAkyüz, Sevim Nur
dc.contributor.authorKina, Umit Yasar
dc.contributor.authorAly, Ahmed S I
dc.contributor.authorPalabiyik, Bedia
dc.date.accessioned2023-05-16T14:32:28Z
dc.date.available2023-05-16T14:32:28Z
dc.date.issued2023-01-16T21:00:00Z
dc.description.abstractIron is an essential cofactor for eukaryotic cells, as well as a toxic metal under certain conditions. On the other hand, glucose is the preferred energy and carbon source by most organisms and is an important signaling molecule in the regulation of biological processes. In Schizosaccharomyces pombe, the Ght5 hexose transporter, known as a high affinity glucose transporter, is required for cell proliferation in low glucose concentrations. Herein, we aimed to investigate the effects of iron stress on the Ght5 hexose transporter under glucose repression and derepression conditions. The effect of iron stress on the expression profile of the ght5 gene was analyzed by RT-qPCR and western blot. The localization of the Ght5-mNeonGreen fusion protein examined with confocal microscopy. Our results revealed that iron stress had an inhibitory effect on ght5 expression, and it altered Ght5 localization on the cell surface, causing it to accumulate in the cytoplasm.
dc.identifier.pubmed36990657
dc.identifier.urihttps://hdl.handle.net/20.500.12645/37821
dc.language.isoen
dc.subjectSchizosaccharomyces pombe
dc.subjectGht5
dc.subjectglucose
dc.subjecthexose transporters
dc.subjectiron stress
dc.subjectmembrane localization
dc.titleIron affects localization of Ght5 in fission yeast.
dspace.entity.typePublication
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