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MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK

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dc.contributor.authorDurmus, ZEHRA
dc.contributor.authorTekkeli, SerIfe Evrim Kepekci
dc.contributor.authorKiziltas, Mustafa Volkan
dc.contributor.authorOnal, Armagan
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2019-10-05T15:15:50Z
dc.date.available2019-10-05T15:15:50Z
dc.date.issued2017-06-01
dc.description.abstractA simple analytical procedure, magnetic solid phase extraction combination to HPLC, has been developed for the analysis of gemifloxacin in human plasma and breast milk. Before chromatographic separation, magnetic solid phase extraction has been applied for sample preparation which is currently preffered extraction technique accordingly its simple, fast and efficient procedure. Fe3O4 magnetic nanoparticles have been used as magnetic adsorbents, the adsorption process has been optimized. RP C18 column has been used with mobile phase composed of acetonitrile-10 mM orthophosphoric acid including 1 mL/L triethylamine (60:40) by isocratic elution with flow rate of 1.3 mL/min. Calibration curve is linear over the range of 0.5-30 mu g/mL and 0.5-20 mu g/mL for plasma and breast milk, respectively. LOD and LOQ has been found to be 0.15 and 0.5 mu g/mL for both matrices. Intraday and interday RSD values are less than 3.57% for both assays. Moreover, the newly developed method provides fast, simple, cost reduced and sensitive assay for gemifloxacin.
dc.description.sponsorshipBezmiâlem Vakıf Üniversitesi
dc.identifier
dc.identifier.citationDurmus Z., Tekkeli S. E. K. , Kiziltas M. V. , Onal A., -MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK-, JOURNAL OF THE CHILEAN CHEMICAL SOCIETY, cilt.62, ss.3483-3489, 2017
dc.identifier.doi10.4067/s0717-97072017000200012
dc.identifier.scopus85027029911
dc.identifier.urihttps://hdl.handle.net/20.500.12645/5843
dc.identifier.wosWOS:000408266600012
dc.language.isoen
dc.titleMAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameTÜRK ÜROLOJİ DERGİSİ
local.avesis.id90eb23b4-ebeb-4938-bb4f-12f38ccdfaa5
local.avesis.response5713
local.publication.isinternational1
relation.isAuthorOfPublication837d5047-762e-43db-b252-9f3d1399378f
relation.isAuthorOfPublication.latestForDiscovery837d5047-762e-43db-b252-9f3d1399378f
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