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Bauhinia bauhinioides cruzipain inhibitor reduces endothelial proliferation and induces an increase of the intracellular Ca2+ concentration

dc.contributor.authorBilgin, MEHMET
dc.contributor.authorNEUHOF, Christiane
dc.contributor.authorDOERR, Oliver
dc.contributor.authorBENSCHEID, Utz
dc.contributor.authorANDRADE, Sheila S.
dc.contributor.authorMOST, Astrid
dc.contributor.authorABDALLAH, Yaser
dc.contributor.authorPARAHULEVA, Mariana
dc.contributor.authorGUENDUEZ, Dursun
dc.contributor.authorOLIVA, Maria L.
dc.contributor.authorERDOGAN, Ali
dc.contributor.institutionauthorBİLGİN, MEHMET
dc.date.accessioned2020-10-29T20:25:08Z
dc.date.available2020-10-29T20:25:08Z
dc.date.issued2010-12-01T00:00:00Z
dc.description.abstractProteinase inhibitors, isolated from different types of Bauhinia, have an effect on apoptosis, angiogenesis and inflammation. The Bauhinia bauhinioides cruzipain inhibitor (BbCI) is a Kunitz-type inhibitor and inactivates the cysteine proteinases cruzipain and cruzain from Trypanosoma cruzi. Cruzipain and tissue kallikrein have similar biochemical properties, e.g. the proteolytic cleavage of the kininogen precursor of lys-bradykinin. Tissue kallikrein stimulation in endothelial cells causes migration and capillary tube formation. The aim of this study was to examine whether the antiproliferative effect of BbCI is dependent on changes of the intracellular calcium concentration and membrane hyperpolarization. Endothelial cells were isolated from human umbilical cord veins (HUVEC). For proliferation experiments, HUVEC were incubated with BbCI (10-100 mu mol/L) for 48 h. The proliferation was detected by cell counting with a Neubauer chamber. The effect of BbCI (10-100 mu M) on the membrane potential was measured with the fluorescence dye DiBAC(4)(3) and the effect on [Ca+2] (i) with the fluorescence probe Fluo-3 AM. The change of the fluorescence intensity was determined with a GENios plate reader (Tecan). The experiments showed that BbCI (10-100 mu mol/L) reduces the endothelial cell proliferation significantly in a concentration-dependent manner with a maximum effect at 100 mu mol/L (35.1 +/- 1.8% as compared to control (p a parts per thousand currency signaEuro parts per thousand 0.05; n = 45)). As compared to the control, the addition of BbCI (100 mu mol/L) caused a significant increase of systolic Ca2+ of 28.4 +/- 5.0% after 30 min incubation. HUVEC treatment with BbCI (100 mu mol/L) showed a weak but significant decrease of the membrane potential of 9.5 +/- 0.9% as compared to control (p a parts per thousand currency signaEuro parts per thousand 0.05; n = 80). BbCI influenced significantly the endothelial proliferation, the intracellular Ca2+ concentration and the membrane potential.
dc.identifier.citationBilgin M., NEUHOF C., DOERR O., BENSCHEID U., ANDRADE S. S. , MOST A., ABDALLAH Y., PARAHULEVA M., GUENDUEZ D., OLIVA M. L. , et al., -Bauhinia bauhinioides cruzipain inhibitor reduces endothelial proliferation and induces an increase of the intracellular Ca2+ concentration-, JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY, cilt.66, ss.283-290, 2010
dc.identifier.doi10.1007/s13105-010-0032-8
dc.identifier.scopus78650170264
dc.identifier.urihttp://hdl.handle.net/20.500.12645/26041
dc.identifier.wosWOS:000284061700002
dc.titleBauhinia bauhinioides cruzipain inhibitor reduces endothelial proliferation and induces an increase of the intracellular Ca2+ concentration
dc.typeArticle
dspace.entity.typePublication
local.avesis.id6862006b-0292-4012-a38f-fcc846df6df8
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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relation.isAuthorOfPublication.latestForDiscoverya64d7f51-ba75-433f-9e99-de1f8528c158
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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