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Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

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dc.contributor.authorGazioglu, IŞIL
dc.contributor.authorKolak, Ufuk
dc.contributor.institutionauthorGAZİOĞLU, IŞIL
dc.date.accessioned2019-10-05T12:53:41Z
dc.date.available2019-10-05T12:53:41Z
dc.date.issued2015-07-01
dc.description.abstractModified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol-water (75+25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.en
dc.description.sponsorshipİstanbul Üniversitesi
dc.description.sponsorshipİstanbul Üniversitesi
dc.identifier
dc.identifier.citationGazioglu I., Kolak U., -Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection-, JOURNAL OF AOAC INTERNATIONAL, cilt.98, ss.939-945, 2015
dc.identifier.doi10.5740/jaoacint.14-211
dc.identifier.pubmed26268976
dc.identifier.scopus84940426486
dc.identifier.urihttps://hdl.handle.net/20.500.12645/847
dc.identifier.wosWOS:000359509500017
dc.rightsinfo:eu-repo/semantics/openAccessen
dc.titleMethod Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameInternational journal of clinical and experimental medicine
local.avesis.id13121769-97e7-40ed-a239-0700f157ca31
local.avesis.response717
local.publication.isinternational1
relation.isAuthorOfPublication336380d2-70ee-496d-a002-08059f9da500
relation.isAuthorOfPublication.latestForDiscovery336380d2-70ee-496d-a002-08059f9da500
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