Publication:
Andrographolide Induces Apoptosis and Cell Cycle Arrest through Inhibition of Aberrant Hedgehog Signaling Pathway in Colon Cancer Cells

Simple item page
dc.contributor.authorKHAN, IMRAN
dc.contributor.authorMahfooz, Sadaf
dc.contributor.authorFaisal, Mohammad
dc.contributor.authorAlatar, Abdulrahman A.
dc.contributor.authorAnsari, Irfan A.
dc.contributor.institutionauthorKHAN, IMRAN
dc.date.accessioned2020-11-11T22:13:54Z
dc.date.available2020-11-11T22:13:54Z
dc.date.issued2020-10-01T00:00:00Z
dc.description.abstractBackground Hedgehog signaling pathway (Hh) is abnormally stimulated in colon cancer. Evidence suggests the therapeutic effectiveness of andrographolide against several cancers. This study attempts to delineate the effect of andrographolide on Hh signaling pathway in colon cancer HCT-116 cells.Methods:Effects of andrographolide were studied on HCT-116 cells by evaluating cytotoxicity by MTT assay, morphology assessment, trypan blue exclusion, and colony formation assay; migratory potential by scratch assay; apoptosis by DAPI, Hoechst staining, FITC-Annexin V assay, and caspases activation; mitochondrial membrane potential (Delta psi m) by Mito Tracker and Rhodamine 123. Intracellular ROS by DCFH-DA staining. Cell cycle regulation by flow cytometry. Expression of BAX, BAD, BCL2, Cyclin B1, CDK1, Smo, and Gli1 by qRT-PCR. Interaction between andrographolide and Smo protein by in-silico molecular docking.Results:Andrographolide induced antiproliferative effect on HCT-116 cells in a dose-dependent and time-dependent manner. It also induced apoptosis and anti-migratory effect in HCT-116 cells. In combination with 5FU, andrographolide exhibited synergistic effect. It Induced G2/M phase arrest through downregulating CDK1 and Cyclin B1. Andrographolide also inhibited Hh signaling by downregulating Smo and Gli1 in HCT-116 cells. It showed high affinity toward Smo protein in-silico.Conclusion:Andrographolide repressed the colon cancer cell growth via inhibiting Hh signaling pathway.
dc.identifier.citationKHAN I., Mahfooz S., Faisal M., Alatar A. A. , Ansari I. A. , -Andrographolide Induces Apoptosis and Cell Cycle Arrest through Inhibition of Aberrant Hedgehog Signaling Pathway in Colon Cancer Cells-, NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2020
dc.identifier.doi10.1080/01635581.2020.1828942
dc.identifier.pubmed33030050
dc.identifier.scopus85092361341
dc.identifier.urihttp://hdl.handle.net/20.500.12645/27369
dc.identifier.wosWOS:000577507700001
dc.titleAndrographolide Induces Apoptosis and Cell Cycle Arrest through Inhibition of Aberrant Hedgehog Signaling Pathway in Colon Cancer Cells
dc.typeArticle
dspace.entity.typePublication
local.avesis.id9cda1537-6ccf-45f6-beaf-4717bddbcde5
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication096be806-cf26-4766-b5b4-26f30c593af2
relation.isAuthorOfPublication.latestForDiscovery096be806-cf26-4766-b5b4-26f30c593af2
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
Files
Collections
Yayınlar - Eserler