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Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma

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dc.contributor.authorUtkusavas, Ayfer
dc.contributor.authorGurevin, Ebru Gurel
dc.contributor.authorYILMAZER, NADİM
dc.contributor.authorÜVEZ, AYÇA
dc.contributor.authorOztay, Fusun
dc.contributor.authorBulut, Huri
dc.contributor.authorÜSTÜNOVA, SAVAŞ
dc.contributor.authorESENER, OSMAN BEHZAT BURAK
dc.contributor.authorSÖNMEZ, KIVILCIM
dc.contributor.authorKutucu, Deniz Erol
dc.contributor.authorMERAL, İSMAİL
dc.contributor.authorDimas, Konstantinos
dc.contributor.authorARMUTAK, Elif İlkay
dc.contributor.institutionauthorÜSTÜNOVA, SAVAŞ
dc.contributor.institutionauthorMERAL, İSMAİL
dc.date.accessioned2022-03-15T20:59:16Z
dc.date.available2022-03-15T20:59:16Z
dc.date.issued2022-03-01T00:00:00Z
dc.description.abstractCombined use of a chemotherapeutic agent and an autophagy inhibitor is a novel cancer treatment strategy. We investigated the effects of chloroquine (CQ) on lung pathology caused by both solid Ehrlich ascites carcinoma (EAC) and doxorubicin (DXR). A control group and eight experimental groups of adult female mice were inoculated subcutaneously with 2.5 x 10(6) EAC cells. DXR (1.5 mg/kg and 3 mg/kg) and CQ (25 mg/kg and 50 mg/kg) alone or in combination were injected intraperitoneally on days 2, 7 and 12 following inoculation with EAC cells. Lung tissue samples were examined using immunohistochemistry (IHC) for endothelial (eNOS), inducible nitric oxide synthase (iNOS) and neutrophil gelatinase-associated lipocalin (NGAL). Serum catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured using ELISA. We found decreased levels of iNOS and eNOS in the groups that received 1.5 mg/kg DXR alone and in combination with 25 mg/kg and 50 mg/kg CQ. Combined administration of DXR and CQ partially prevented disruption of alveolar structure. Levels of antioxidant enzymes and MDA were lower in all treated groups; the greatest reduction was observed in mice that received the combination of 25 mg/kg CQ + 1.5 mg/kg DXR. Levels of NGAL were elevated in all treated groups. We found that CQ ameliorated both EAC and DOX induced lung pathology in female mice with solid EAC by reducing oxidative stress.
dc.identifier.citationUtkusavas A., Gurevin E. G. , YILMAZER N., ÜVEZ A., Oztay F., Bulut H., ÜSTÜNOVA S., ESENER O. B. B. , SÖNMEZ K., Kutucu D. E. , et al., -Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma-, BIOTECHNIC & HISTOCHEMISTRY, 2022
dc.identifier.doi10.1080/10520295.2022.2036369
dc.identifier.pubmed35240890
dc.identifier.urihttp://hdl.handle.net/20.500.12645/30446
dc.identifier.wosWOS:000764954500001
dc.titleEffects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma
dc.typeArticle
dspace.entity.typePublication
local.avesis.id3481721d-f20b-45c9-af56-26c773f89581
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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relation.isAuthorOfPublication.latestForDiscovery58058ee9-1440-480c-9817-c6b25d7ec008
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relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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