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A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study

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dc.contributor.authorTEKKELİ, ŞERİFE EVRİM
dc.contributor.authorCeylan, Burhan
dc.contributor.authorTIRIS, GİZEM
dc.contributor.institutionauthorTIRIS, GİZEM
dc.contributor.institutionauthorTEKKELİ, ŞERİFE EVRİM
dc.date.accessioned2021-08-17T20:59:05Z
dc.date.available2021-08-17T20:59:05Z
dc.date.issued2021-09-01T00:00:00Z
dc.description.abstractThis article aims to present a simple and sensitive, HPLC-UV method, which was developed to determine carnosol in human plasma samples. Chromatographic separation was achieved with C18 column (150 mm x 4.6 mm x 5 mu m), at 25 degrees C with gradient elution of the mobile phase consisting of methanol-water (2% o-phosphoric acid) at flow rate 1.2 mL/min. The analyte was detected at 230 nm by UV detector. The retention time of carnosol is 3.40 +/- 0.01 min. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 1-20 ng/mL with the correlation coefficient of 0.9942. The proposed method was applied successfully to the analysis of carnosol in spiked human plasma with good recovery as 96.4% and the precision of the method was determined by intra day and interday assays with the highest RSD % values 5.71. The method successfully applied to a pharmacokinetic study with determination of C-max, t(max), t(1/2) and AUC, by administration of carnosol to a healthy volunteer.
dc.identifier.citationCeylan B., TIRIS G., TEKKELİ Ş. E. , -A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study-, CHROMATOGRAPHIA, cilt.84, sa.9, ss.855-860, 2021
dc.identifier.doi10.1007/s10337-021-04069-0
dc.identifier.scopus85111747043
dc.identifier.urihttp://hdl.handle.net/20.500.12645/29252
dc.identifier.wosWOS:000681192200001
dc.titleA New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study
dc.typeArticle
dspace.entity.typePublication
local.avesis.id2255fd99-bb5f-436c-a2f4-b9dfaa1660a7
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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