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Chitosan and carboxymethyl cellulose based magnetic nanocomposites for application of peroxidase purification

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dc.contributor.authorKurt, BELMA
dc.contributor.authorUckaya, FATİH
dc.contributor.authorDurmus, ZEHRA
dc.contributor.institutionauthorZENGİN KURT, BELMA
dc.contributor.institutionauthorUÇKAYA, FATİH
dc.date.accessioned2019-10-05T14:24:48Z
dc.date.available2019-10-05T14:24:48Z
dc.date.issued2017-03-01
dc.description.abstractRecently, protein purification methods have a very wide area of research. Many of these methods are both expensive and multi-stage methods, that are needed in specific equipment. In this study, biopolymer coated magnetic nanoparticles, carboxymethyl cellulose (CMC) and chitosan (CH) coated Fe3O4 (magnetite) nanocomposites, are used in a new purification process. The structure of the synthesized magnetic nanocomposites were characterized by Fourier transform infrared (FTIR) spectrometry, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric (TGA) analysis and zeta potential for surface charge of magnetic nanocomposites. Molecular weight and purification degree of peroxidase were estimated with SDS-PAGE. Peroxidase enzyme was purified a yield of 82.55% with carboxymethyl cellulose and 76.72% with chitosan using this method. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier
dc.identifier.citationKurt B., Uckaya F., Durmus Z., -Chitosan and carboxymethyl cellulose based magnetic nanocomposites for application of peroxidase purification-, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.96, ss.149-160, 2017
dc.identifier.pubmed27988290
dc.identifier.urihttps://hdl.handle.net/20.500.12645/3813
dc.language.isoen
dc.titleChitosan and carboxymethyl cellulose based magnetic nanocomposites for application of peroxidase purification
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameAdvances in peritoneal dialysis. Conference on Peritoneal Dialysis
local.avesis.id5c716c3d-066a-4eeb-b0dc-29578e748f69
local.avesis.response3683
local.org.facultyTıp Fakültesi
relation.isAuthorOfPublication3fd265b5-fdd8-4c56-949b-deb0303c3db7
relation.isAuthorOfPublicationd456484b-f806-4669-8385-1bf18014746e
relation.isAuthorOfPublication.latestForDiscoveryd456484b-f806-4669-8385-1bf18014746e
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