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Effects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells

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dc.contributor.authorSalkin, Hasan
dc.contributor.authorGÖNEN, ZEYNEP BURÇİN
dc.contributor.authorÖZCAN, SERVET
dc.contributor.authorLekesizcan, Ayca
dc.contributor.authorBAHAR, DİLEK
dc.contributor.authorKÜTÜK, NÜKHET
dc.contributor.authorALKAN, ALPER
dc.contributor.institutionauthorKÜTÜK, NÜKHET
dc.contributor.institutionauthorALKAN, ALPER
dc.date.accessioned2019-12-29T20:59:43Z
dc.date.available2019-12-29T20:59:43Z
dc.date.issued2019-10-13
dc.description.abstractAim: The aim of this study was to evaluate the effects of standard culture medium and chondrogenic differentiation medium with PRP on chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells (rabbit DPSCs) that are transfected with transforming growth factor-beta 1 (TGF-B1) gene, based on the hypothesis of TGF- B1 and PRP can be effective on the chondrogenesis of stem cells. Materials and Methods: Rabbit DPSCs were characterized by using flow cytometry, immunofluorescent staining, quantitative Real Time Polymerase Chain Reaction (qRT-PCR) and differentiation tests. For the characterization, CD29, CD44 and CD45 mesenchymal cell markers were used. Rabbit DPSCs were transfected with TGF-B1 gene using electroporation technique in group 1; with PRP 10% in group 2; with chondrogenic medium in group 3; with both chondrogenic medium and PRP in group 4. DPSCs were cultured in medium with 10% inactive PRP in group 5, chondrogenic medium in group 6, chondrogenic medium with PRP 10% in group 7. SOX9, MMP13 and Aggrecan gene expression levels were evaluated in 3, 6, 12. and 24. days by qRT-PCR. Results: The expression levels of SOX9, MMP13 and Aggrecan were higher in group 2, 3 and group 7 in 3th day however in 24th day group 7 and group 2 were found higher. The expression levels changed by time-dependent. The extracellular matrix of the cells in experimental groups were positively stained with safranin O and toluidine blue. Conclusion: The combination in culture medium of TGF-B1 gene transfection and 10% PRP accelerates the chondrogenic differentiation of DPSCs.
dc.identifier.citationSalkin H., GÖNEN Z. B. , ÖZCAN S., BAHAR D., Lekesizcan A., TAHERİ S., KÜTÜK N., ALKAN A., -Effects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells-, CONNECTIVE TISSUE RESEARCH, 2019
dc.identifier.pubmed31581853
dc.identifier.urihttps://hdl.handle.net/20.500.12645/11118
dc.titleEffects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublication908c31f8-027f-44c3-9992-1f8b42ac3367
relation.isAuthorOfPublicationf621b6f2-1415-4076-b3a2-78835a8712bd
relation.isAuthorOfPublication.latestForDiscoveryf621b6f2-1415-4076-b3a2-78835a8712bd
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