Publication:
Point Mutations at gyrA and gyrB Genes of Levofloxacin Resistant Helicobacter pylori Strains and Dual Resistance with Clarithromycin

dc.contributor.authorZiver-Sarp, Tevhide
dc.contributor.authorYÜKSEL MAYDA, PELİN
dc.contributor.authorSaribas, Suat
dc.contributor.authorDEMİRYAS, Süleyman
dc.contributor.authorGareayaghi, Nesrin
dc.contributor.authorERGİN, Sevgi
dc.contributor.authorTAŞÇI, İhsan
dc.contributor.authorOzbey, Dogukan
dc.contributor.authorBAL, Kadir
dc.contributor.authorErzin, Yusuf
dc.contributor.authorAKKUŞ, Seher
dc.contributor.authorBAHAR TOKMAN, Hrisi
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorTufan-Kocak, Banu
dc.contributor.authorKOCAZEYBEK, Bekir Sami
dc.contributor.institutionauthorYÜKSEL MAYDA, PELİN
dc.date.accessioned2021-11-02T20:59:17Z
dc.date.available2021-11-02T20:59:17Z
dc.date.issued2021-01-01T00:00:00Z
dc.description.abstractBackground: Spontaneous point mutations in genes encoding gyrA/B subunits of DNA gyrase are responsible for fluoroquinolone resistance. We aimed to determine the clarithromycin and levofloxacin resistance phenotypically in H. pylori strains and to investigate the mutations responsible for levofloxacin resistance and the effects of these mutations on dual antibiotic resistance. Methods: A total of 65 H. pylori isolates were included. The E-test method was used for the clarithromycin and le-vofloxacin antimicrobial susceptibility test. Real-time PCR was used to detect the point mutations. Results: Twenty-four (36.9%) of 65 H. pylori strains were phenotypically resistant to clarithromycin and 14 (21.5%) to levofloxacin. The phenotypic levofloxacin resistance rate of strains with Asn87Lys and Asp91Asn mu-tations were significantly higher (gyrA gene) (p < 0.05). The phenotypic levofloxacin resistance rate of strains with Arg484Lys and Asp481Glu mutations were significantly higher (gyrB gene) (p < 0.05). The Asn87Lys mutation in-creased the risk of phenotypes being resistant to levofloxacin 70.156 times and Asp91Asn mutation increased 125,427 times higher. Seven (10.8%) of 65 H. pylori strains showed dual resistance to both levofloxacin and cla-rithromycin. The rate of being dual resistant with A2143G mutation (clarithromycin resistance) was found to be significantly higher (p < 0.05). Conclusions: The Asn87Lys and Asp91Asn mutations in the gyrA gene had a phenotypically enhancing effect on levofloxacin resistance, while the presence of Asp481Glu and Arg484Lys mutations in the gyrB gene did not. The existence of dual resistance was developed with the increase in clarithromycin and levofloxacin resistance rates. (Clin. Lab. 2021;67:2369-2377. DOI: 10.7754/Clin.Lab.2021.210843)
dc.identifier.citationZiver-Sarp T., YÜKSEL MAYDA P., Saribas S., DEMİRYAS S., Gareayaghi N., ERGİN S., TAŞÇI İ., Ozbey D., BAL K., Erzin Y., et al., -Point Mutations at gyrA and gyrB Genes of Levofloxacin Resistant Helicobacter pylori Strains and Dual Resistance with Clarithromycin-, CLINICAL LABORATORY, cilt.67, sa.10, ss.2369-2377, 2021
dc.identifier.doi10.7754/clin.lab.2021.210843
dc.identifier.urihttp://hdl.handle.net/20.500.12645/29611
dc.identifier.wosWOS:000707662400027
dc.titlePoint Mutations at gyrA and gyrB Genes of Levofloxacin Resistant Helicobacter pylori Strains and Dual Resistance with Clarithromycin
dc.typeArticle
dspace.entity.typePublication
local.avesis.id55164e3d-f928-498e-945d-f28ccd4f4918
local.publication.isinternational1
relation.isAuthorOfPublicationa7da1ef6-442b-46fc-8c6c-601442ec1778
relation.isAuthorOfPublication.latestForDiscoverya7da1ef6-442b-46fc-8c6c-601442ec1778
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