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Effect of metformin on the human T98G glioblastoma multiforme cell line

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dc.contributor.authorUcbek, Ali
dc.contributor.authorOzunal, Zeynep Gunes
dc.contributor.authorUzun, Ozge
dc.contributor.authorGepdiremen, ALİ AKÇAHAN
dc.contributor.institutionauthorGEPDİREMEN, ALİ AKÇAHAN
dc.date.accessioned2020-10-29T14:39:36Z
dc.date.available2020-10-29T14:39:36Z
dc.date.issued2014-05-01T00:00:00Z
dc.description.abstractMetformin is a guanidine derivative found in Galega officinalis that is commonly used to treat diabetes mellitus. The mechanism of action of metformin involves regulation of the adenosine monophosphate-activated protein kinase/mammalian target of rapamycin signaling pathway, which is implicated in the control of protein synthesis and cell proliferation. This led to the hypothesis that metformin reduces the risk of cancer and slows tumor growth. Thus, in the present study, the effectiveness of metformin as an antiglioma agent was evaluated using the human T98G glioblastoma multiforme cell line. The viability of the T98G cells was assessed using a 3-(4,5-dimethylthiazol-2-yL)-2,5-diphenyltetrazolium bromide assay. Apoptosis was monitored by measuring caspase-3 levels, as well as by terminal deoxynucleotidyl transferase dUTP nick end labeling and staining with acridine orange and ethidium bromide. The results demonstrate that metformin reduced cell viability and caused apoptotic morphological changes in the T98G cells. Furthermore, the caspase-3 levels in the metformin-treated T98G cells were higher than those in the control cells. Metformin induced apoptosis in the T98G cell line in a concentration-dependent manner. Metformin may provide an important contribution to the treatment of glioblastoma multiforme.
dc.identifier.citationUcbek A., Ozunal Z. G. , Uzun O., Gepdiremen A. A. , -Effect of metformin on the human T98G glioblastoma multiforme cell line-, EXPERIMENTAL AND THERAPEUTIC MEDICINE, cilt.7, ss.1285-1290, 2014
dc.identifier.doi10.3892/etm.2014.1597
dc.identifier.scopus84896838154
dc.identifier.trdizintrdizin
dc.identifier.urihttp://hdl.handle.net/20.500.12645/25362
dc.identifier.wosWOS:000337557700040
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleEffect of metformin on the human T98G glioblastoma multiforme cell line
dc.typeArticle
dspace.entity.typePublication
local.avesis.id280d86f2-1174-4259-a807-5010103f2b57
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublicationdc70adc1-eb72-4820-8b6d-4b39285a568f
relation.isAuthorOfPublication.latestForDiscoverydc70adc1-eb72-4820-8b6d-4b39285a568f
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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