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Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe

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dc.contributor.authorKina, Umit Yasar
dc.contributor.authorPalabiyik, Bedia
dc.contributor.institutionauthorKINA, ÜMİT YAŞAR
dc.date.accessioned2019-10-05T14:49:20Z
dc.date.available2019-10-05T14:49:20Z
dc.date.issued2019-01-01
dc.description.abstractHydrogen peroxide is an agent that triggers oxidative stress. Glucose, which is a source of carbon and energy has a regulatory role in many metabolic processes such as growth rate, fermentation capacity and stress response. Schizosaccharomyces pombe has eight hexose transporters with a different affinity for glucose and/or related monosaccharides. In S. pombe. Ght5 is a glucose transporter with high-affinity. We aimed to investigate the effects of H2O2-induced oxidative stress on hexose transporters using glucose repression-resistant mutant strains (ird5 and ird11) of S. pombe. We analyzed the percentage of glucose consumption in S. pombe wild-type and mutant cells under stressed and non-stressed conditions. Then we compared the expression levels of the genes encoding hexose transporters under the same conditions. We confirmed that the glucose consumption efficiencies of the mutants were slower than the wild-type as in earlier study under non-stressed condition. The percentage of glucose consumption reduced by approximately two-fold in ird11 and wild-type, but not change in ird5, under a stressed condition. There is no difference between cells shape and size of S. pombe strains under stressed and non-stressed conditions. Under stress-induced condition, the expression levels of ght3, ght4 genes in ird11 and wild-type, and ght4, ght6 genes in ird5 decreased, but that of ght5 gene remarkably increased in only wild-type. We suggested that oxidative stress caused by H2O2 leads to upregulation of the ght5 gene in S. pombe.
dc.description.sponsorshipİstanbul Üniversitesi
dc.identifier10.1177/1078155219839460
dc.identifier.citationKina U. Y. , Palabiyik B., -Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe-, CELLULAR AND MOLECULAR BIOLOGY, cilt.65, ss.41-45, 2019
dc.identifier.pubmed30782292
dc.identifier.urihttps://hdl.handle.net/20.500.12645/4785
dc.language.isoen
dc.titleHydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameJournal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners
local.avesis.id75a1c6ce-2cce-4be3-9b73-ddf9d4a30b74
local.avesis.response4655
relation.isAuthorOfPublicationc3765050-04eb-4a05-9f88-b9913d0f1f06
relation.isAuthorOfPublication.latestForDiscoveryc3765050-04eb-4a05-9f88-b9913d0f1f06
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