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Genetic Characterization of Plasmodium Putative Pantothenate Kinase Genes Reveals Their Essential Role in Malaria Parasite Transmission to the Mosquito

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dc.contributor.authorHart, Robert J.
dc.contributor.authorCornillot, Emmanuel
dc.contributor.authorAbraham, Amanah
dc.contributor.authorMolina, Emily
dc.contributor.authorNation, Catherine S.
dc.contributor.authorBen Mamoun, Choukri
dc.contributor.authorAly, Ahmed Sayed Ibrahım
dc.contributor.institutionauthorALY, AHMED SAYED IBRAHıM
dc.date.accessioned2020-10-22T19:28:14Z
dc.date.available2020-10-22T19:28:14Z
dc.date.issued2016-09-20T00:00:00Z
dc.description.abstractThe metabolic machinery for the biosynthesis of Coenzyme A (CoA) from exogenous pantothenic acid (Vitamin B-5) has long been considered as an excellent target for the development of selective antimicrobials. Earlier studies in the human malaria parasite Plasmodium falciparum have shown that pantothenate analogs interfere with pantothenate phosphorylation and block asexual blood stage development. Although two eukaryotic-type putative pantothenate kinase genes (PanK1 and PanK2) have been identified in all malaria parasite species, their role in the development of Plasmodium life cycle stages remains unknown. Here we report on the genetic characterization of PanK1 and PanK2 in P. yoelii. We show that P. yoelii parasites lacking either PanK1 or PanK2 undergo normal asexual stages development and sexual stages differentiation, however they are severely deficient in ookinete, oocyst and sporozoite formation inside the mosquito vector. Quantitative transcriptional analyses in wild-type and knockout parasites demonstrate an important role for these genes in the regulation of expression of other CoA biosynthesis genes. Together, our data provide the first genetic evidence for the importance of the early steps of pantothenate utilization in the regulation of CoA biosynthesis and malaria parasite transmission to Anopheles mosquitoes.
dc.identifier.citationHart R. J. , Cornillot E., Abraham A., Molina E., Nation C. S. , Ben Mamoun C., Aly A. S. I. , -Genetic Characterization of Plasmodium Putative Pantothenate Kinase Genes Reveals Their Essential Role in Malaria Parasite Transmission to the Mosquito-, SCIENTIFIC REPORTS, cilt.6, 2016
dc.identifier.doi10.1038/srep33518
dc.identifier.scopus84988649528
dc.identifier.urihttp://hdl.handle.net/20.500.12645/24527
dc.identifier.wosWOS:000383489300001
dc.titleGenetic Characterization of Plasmodium Putative Pantothenate Kinase Genes Reveals Their Essential Role in Malaria Parasite Transmission to the Mosquito
dc.typeArticle
dspace.entity.typePublication
local.avesis.idc278d11a-d762-4d79-86c0-4cc3f2f7b333
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication47a38002-6a0b-4c36-b83d-773a943d999d
relation.isAuthorOfPublication.latestForDiscovery47a38002-6a0b-4c36-b83d-773a943d999d
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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