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Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.

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dc.contributor.authorKucukkaya, B
dc.contributor.authorBasoglu, H
dc.contributor.authorErdag, D
dc.contributor.authorAkbas, FAHRİ
dc.contributor.authorYalcintepe, L
dc.contributor.institutionauthorAKBAŞ, FAHRİ
dc.contributor.institutionauthorSÜSGÜN, SEDA
dc.date.accessioned2019-10-05T23:19:13Z
dc.date.available2019-10-05T23:19:13Z
dc.date.issued2019-07-01
dc.description.abstractIntracellular calcium concentration ([Ca2+](i)) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high success rate in cancer therapies. We investigated the regulatory role of [Ca2+]in cisplatin resistance in epithelial ovarian cancer cell line, in MDAH-2774, and its chemoresistant subclone MDAH-2774/DDP. The measurement of [Ca2+](i) using fluorescence microscope, and flow cytometry revealed that the amount of intracellular calcium decreased in cisplatin resistant cells compared to the amounts in parental cells. mRNA expression profiles of calcium homeostasis-associated major genes (IP(3)R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) decreased in cisplatin resistant cell line in comparison to the expression profiles in parental cells. Owing to the changes in the expression of genes involved in calcium regulation, these results show, drug resistance may be prevented by introducing a new perspective on the use of inhibitors and activators of these genes, and thus of cytostatic treatment strategies, due to changes in the expression of genes involved in calcium regulation.
dc.identifier
dc.identifier.citationKucukkaya B., Basoglu H., Erdag D., Akbas F., Susgun S., Yalcintepe L., -Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.-, General physiology and biophysics, cilt.38, ss.353-363, 2019
dc.identifier.doi10.4149/gpb_2019013
dc.identifier.pubmed31241042
dc.identifier.urihttps://hdl.handle.net/20.500.12645/9991
dc.language.isoen
dc.titleCalcium homeostasis in cisplatin resistant epithelial ovarian cancer.
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameUlusal Travma Dergisi
local.avesis.idf991bfca-a25c-499a-8ab0-9e71f019f1dc
local.avesis.response9865
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
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relation.isAuthorOfPublication.latestForDiscoveryc630e619-3437-4b50-940e-8aef11c5388c
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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