Biochemical, Histopathologic, and Genotoxic Effects of Ethanol Extract of (Fisch. & Mey.) on Incisional and Excisional Wounded Diabetic Rats.

Abstract

: Nonhealing wounds are a serious problem of diabetic patients. species are traditionally used for the treatment of wounds. The aim of the study was to investigate the effects of ointment prepared with ethanol extract obtained from the aerial parts of , an endemic plant from Turkey, on diabetic rat incisional and excisional skin wounds. : Male Wistar albino rats (: 60) were divided into five groups. Diabetes was induced and two concentrations (0.5% and 1%) of the extract were used for ointments and applied on wounds for 7 and 14 days. Fito cream was chosen as a reference drug. : In excisional wounds, healing ratios of 0.5% (63.4% and 99.3%) and 1% (65.5% and 99.9%) groups were higher compared to control (35.9% and 75.1%), and in incisional wounds, healing ratios of 0.5% (78.1% and 98.5%) and 1% (84.4% and 99.4%) groups were higher compared to control (30.5% and 72.9%) ( < .01). Hydroxyproline (0.31 ± 0.3 and 0.34 ± 0.2) levels were lower and GSH (10.7 ± 3.1 and 7.6 ± 0.9) levels were higher in 0.5% and 1% groups on the 14th day ( < .01). Histopathological results revealed re-epithelialization and formation of granulation tissue in all groups. Genotoxicologic results indicated, GDI, DCP values, and MN frequency of 0.5% and 1% groups did not reach to significant levels both on the 7 and 14 days. : may have a potential for therapeutic use in treatment and management of diabetic wounds with a successful topical application.