Development of a green high-performance liquid chromatography method for tofacitinib quantification in pharmaceutical formulations and degradation studies

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Genc A. A.
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A new high-performance liquid chromatography (HPLC) method was applied for the quantification of the active substance of tofacitinib. Analysis was performed on a Chromasil 100 C18 (100.0 x 4.0 mm, 3.5 mu m) stationary phase. The mobile phase consisted of acetonitrile:0.2% phosphoric acid in water (12:88, v/v). The prepared sample (20.0 mu L) was injected into the system. A detection wavelength of 285.0 nm was chosen for the compound, and the flow rate was 0.8 mL/min. The experiment was completed in 5.0 min. The analysis temperature was set to 40.0 degrees C. The method was evaluated using green chemistry. The method was validated according to the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use guidelines. For linearity studies calibration curves were constructed in the range of 10.0-200.0 mu g/mL. The recovery values were calculated at 97.66% and 105.68%. The method developed for the analysis of the active substance had a short analysis time and was cost-effective. It is an environmentally friendly method due to the mobile phase content used. The technique can be used in laboratory analysis and bioequivalence experiments.
Tıp, Eczacılık, Temel Eczacılık Bilimleri, Yaşam Bilimleri, Biyokimya, Moleküler Biyoloji ve Genetik, Sitogenetik, Kimya, Analitik Kimya, Sağlık Bilimleri, Temel Tıp Bilimleri, Temel Bilimler, Medicine, Pharmacology and Therapeutics, Basic Pharmaceutics Sciences, Life Sciences, Biochemistry, Molecular Biology and Genetics, Cytogenetic, Chemistry, Analytical Chemistry, Health Sciences, Fundamental Medical Sciences, Natural Sciences, BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ, Biyoloji ve Biyokimya, Yaşam Bilimleri (LIFE), BİYOKİMYA VE MOLEKÜLER BİYOLOJİ, KİMYA, ANALİTİK, Temel Bilimler (SCI), FARMAKOLOJİ VE ECZACILIK, Farmakoloji ve Toksikoloji, BIOCHEMICAL RESEARCH METHODS, BIOLOGY & BIOCHEMISTRY, Life Sciences (LIFE), BIOCHEMISTRY & MOLECULAR BIOLOGY, MOLECULAR BIOLOGY & GENETICS, CHEMISTRY, ANALYTICAL, CHEMISTRY, Natural Sciences (SCI), PHARMACOLOGY & PHARMACY, PHARMACOLOGY & TOXICOLOGY, Farmakoloji, İlaç Keşfi, Farmakoloji, Toksikoloji ve Eczacılık (çeşitli), Genel Farmakoloji, Toksikoloji ve Eczacılık, Farmakoloji (tıbbi), İlaç Rehberleri, Biyokimya (tıbbi), Kimya (çeşitli), Genel Kimya, Filtrasyon ve Ayırma, Yapısal Biyoloji, Moleküler Biyoloji, Klinik Biyokimya, Kanser Araştırmaları, Yaşlanma, Biyokimya, Genetik ve Moleküler Biyoloji (çeşitli), Genel Biyokimya, Genetik ve Moleküler Biyoloji, Fizik Bilimleri, Pharmacy, Pharmacology, Drug Discovery, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), General Pharmacology, Toxicology and Pharmaceutics, Pharmacology (medical), Drug Guides, Biochemistry (medical), Chemistry (miscellaneous), General Chemistry, Filtration and Separation, Structural Biology, Molecular Biology, Clinical Biochemistry, Cancer Research, Aging, Biochemistry, Genetics and Molecular Biology (miscellaneous), General Biochemistry, Genetics and Molecular Biology, Physical Sciences
TIRIS G., Genc A. A., ERK N., "Development of a green high-performance liquid chromatography method for tofacitinib quantification in pharmaceutical formulations and degradation studies", BIOMEDICAL CHROMATOGRAPHY, 2024
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