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Plasmodium yoelii Vitamin B-5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito

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dc.contributor.authorHart, Robert J.
dc.contributor.authorLawres, Lauren
dc.contributor.authorFritzen, Emma
dc.contributor.authorBen Mamoun, Choukri
dc.contributor.authorAly, Ahmed Sayed Ibrahım
dc.contributor.institutionauthorALY, AHMED SAYED IBRAHıM
dc.date.accessioned2020-10-22T15:59:21Z
dc.date.available2020-10-22T15:59:21Z
dc.date.issued2014-07-11T00:00:00Z
dc.description.abstractIn nearly all non-photosynthetic cells, pantothenate (vitamin B-5) transport and utilization are prerequisites for the synthesis of the universal essential cofactor Coenzyme A (CoA). Early studies showed that human malaria parasites rely on the uptake of pantothenate across the parasite plasma membrane for survival within erythrocytes. Recently, a P. falciparum candidate pantothenate transporter (PAT) was characterized by functional complementation in yeast. These studies revealed that PfPAT mediated survival of yeast cells in low pantothenate concentrations and restored sensitivity of yeast cells lacking pantothenate uptake to fenpropimorph. In addition, PfPAT was refractory to deletion in P. falciparum in vitro, but nothing is known about the in vivo functions of PAT in Plasmodium life cycle stages. Herein, we used gene-targeting techniques to delete PAT in Plasmodium yoelii. Parasites lacking PAT displayed normal asexual and sexual blood stage development compared to wild-type (WT) and WT-like p230p(-) parasites. However, progression from the ookinete to the oocyst stage and sporozoite formation were completely abolished in pat(-) parasites. These studies provide the first evidence for an essential role of a candidate pantothenate transport in malaria transmission to Anopheles mosquitoes. This will set the stage for the development of PAT inhibitors against multiple parasite life cycle stages.
dc.identifier.citationHart R. J. , Lawres L., Fritzen E., Ben Mamoun C., Aly A. S. I. , -Plasmodium yoelii Vitamin B-5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito-, SCIENTIFIC REPORTS, cilt.4, 2014
dc.identifier.doi10.1038/srep05665
dc.identifier.scopus84904358183
dc.identifier.urihttp://hdl.handle.net/20.500.12645/23797
dc.identifier.wosWOS:000338828500006
dc.titlePlasmodium yoelii Vitamin B-5 Pantothenate Transporter Candidate is Essential for Parasite Transmission to the Mosquito
dc.typeArticle
dspace.entity.typePublication
local.avesis.id5e3e3d0d-d01a-425a-9e4d-fd0f102a10b2
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
local.publication.isinternational1
relation.isAuthorOfPublication47a38002-6a0b-4c36-b83d-773a943d999d
relation.isAuthorOfPublication.latestForDiscovery47a38002-6a0b-4c36-b83d-773a943d999d
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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