Publication:
Development of eco-friendly sensitive HPLC method for determination of Letrozole and assessment of validation

dc.contributor.authorLotfy H. M.
dc.contributor.authorGenc A. A.
dc.contributor.authorBAY M.
dc.contributor.authorTIRIS G.
dc.contributor.authorObaydo R. H.
dc.contributor.authorERK N.
dc.date.accessioned2025-03-26T21:50:24Z
dc.date.issued2025-08-01
dc.description.abstractThe greening of analytical methods has gained interest in the field of quantitative analysis to reduce environmental impact and improve analyst\"s safety and health. For postmenopausal women with hormone-responsive advanced breast cancer, Letrozole (LTZ) has been a standard first-line endocrine therapy A green HPLC method was developed, optimized, validated, and applied for quantification of the LTZ in tablets. Experiments were conducted on the Inertsil ODS- 3® C18 (5 µm, 150 × 4.6 mm). An isocratic elution was applied using a mobile phase consisting of ethanol: water (50:50, v/v). A wavelength of 230.0 nm was chosen for the analysis. An experiment was finished 3.0 mins. Calibration graphs for each drug were rectilinear in the range of 0.1 – 40.0 µg/mL. Validation studies were carried out based on International Conference on Harmonization (ICH) recommendations. The student (t) test for means and the (F) test for standard deviations were used to compare the results. In this study, we utilized the ChlorTox Scale, Greenness Index, AGREE, GAPI, and NQS Indicator to comprehensively evaluate the environmental, safety, and sustainability aspects of analytical methodology, aligning our approach with the United Nations Sustainable Development Goals (SDGs). It can be applied to quality control, bioequivalence studies, and routine analysis.
dc.identifier.citationLotfy H. M., Genc A. A., BAY M., TIRIS G., Obaydo R. H., ERK N., "Development of eco-friendly sensitive HPLC method for determination of Letrozole and assessment of validation", Talanta Open, cilt.11, 2025
dc.identifier.doi10.1016/j.talo.2025.100425
dc.identifier.issn2666-8319
dc.identifier.scopus85219546239
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85219546239&origin=inward
dc.identifier.urihttps://hdl.handle.net/20.500.12645/40495
dc.identifier.volume11
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKimya
dc.subjectAnalitik Kimya
dc.subjectTemel Bilimler
dc.subjectChemistry
dc.subjectAnalytical Chemistry
dc.subjectNatural Sciences
dc.subjectTemel Bilimler (Sci)
dc.subjectKimya Analitik
dc.subjectNatural Sciences (Sci)
dc.subjectChemistry Analytical
dc.subjectFizik Bilimleri
dc.subjectPhysical Sciences
dc.subjectGreen chemistry
dc.subjectHPLC
dc.subjectLetrozole
dc.subjectQuantification
dc.subjectSustainable development goals (SDGs)
dc.titleDevelopment of eco-friendly sensitive HPLC method for determination of Letrozole and assessment of validation
dc.typearticle
dspace.entity.typePublication
local.avesis.ida0dcf0c1-016d-445c-8115-5cbfad35c522

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