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Polymer-Albumin Conjugate for the Facilitated Delivery of Macromolecular Platinum Drugs

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dc.contributor.authorDag, AYDAN
dc.contributor.authorJIANG, Yanyan
dc.contributor.authorABD KARIM, Khairil Juhanni
dc.contributor.authorHART-SMITH, Gene
dc.contributor.authorSCARANO, Wei
dc.contributor.authorSTENZEL, Martina H.
dc.contributor.institutionauthorDAĞ, AYDAN
dc.date.accessioned2019-10-05T21:23:39Z
dc.date.available2019-10-05T21:23:39Z
dc.date.issued2015-05-01
dc.description.abstractThe delivery of macromolecular platinum drugs into cancerous cells is enhanced by conjugating the polymer to albumin. The monomers N-(2-hydroxypropyl)methacrylamide (HPMA) and Boc protected 1,3-diaminopropan-2-yl acrylate (Ac-DAP-Boc) are copolymerized in the presence of a furan protected maleimide functionalized reversible addition-fragmentation chain transfer (RAFT) agent. The resulting polymer with a composition of P(HPMA(14)-co-(Ac-DAP-Boc)(9)) and a molecular weight of M-n = 7600 g mol(-1) (D = 1.24) is used as a macromolecular ligand for the conjugation to the platinum drug. Thermogravimetric analysis reveals full conjugation. After deprotection of the maleimide functionality of the polymer, the reactive polymer is conjugated to albumin using the Cys34 functionality. The conjugation is monitored using size exclusion chromatography, MALDI-TOF (matrix assisted laser desorption ionization time-of-flight), and SDS Page (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The polymer-albumin conjugates self-assemble in water into nanoparticles of sizes of around 80 nm thanks to the hydrophobic nature of the platinum drugs. The albumin coated nanoparticles are readily taken up by ovarian cancer cell lines and they show superior toxicity compared to a control sample without protein coating.
dc.description.sponsorshipTürkiye Bilimsel Ve Teknolojik Araştırma Kurumu ( Tübitak )
dc.identifier10.1007/s00595-012-0433-0
dc.identifier.citationDag A., JIANG Y., ABD KARIM K. J. , HART-SMITH G., SCARANO W., STENZEL M. H. , -Polymer-Albumin Conjugate for the Facilitated Delivery of Macromolecular Platinum Drugs-, MACROMOLECULAR RAPID COMMUNICATIONS, cilt.36, ss.890-897, 2015
dc.identifier.pubmed25790077
dc.identifier.urihttps://hdl.handle.net/20.500.12645/7190
dc.language.isoen
dc.titlePolymer-Albumin Conjugate for the Facilitated Delivery of Macromolecular Platinum Drugs
dc.typeArticle
dspace.entity.typePublication
local.article.journalnameSurgery today
local.avesis.idb31ee611-9b98-4c9c-a847-bf58d38b8093
local.avesis.response7066
local.publication.goal03 - Sağlık ve Kaliteli Yaşam
relation.isAuthorOfPublication5053fdfd-57cd-423a-9076-db4bab8cbe3d
relation.isAuthorOfPublication.latestForDiscovery5053fdfd-57cd-423a-9076-db4bab8cbe3d
relation.isGoalOfPublication9c198c48-b603-4e2f-8366-04edcfc1224c
relation.isGoalOfPublication.latestForDiscovery9c198c48-b603-4e2f-8366-04edcfc1224c
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