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KINA, ÜMİT YAŞAR

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ÜMİT YAŞAR
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KINA
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Now showing 1 - 6 of 6
  • PublicationMetadata only
    Genetic disruption of nucleoside transporter 4 reveals its critical roles in malaria parasite sporozoite functions.
    (2022-08-24T00:00:00Z) Deveci, GÖZDE; Kamil, Mohd; Kina, Umit; Temel, Binnur Aydogan; Aly, Ahmed S I; DEVECİ, GÖZDE; KAMIL, MOHD; KINA, ÜMİT YAŞAR; TEMEL, BİNNUR
  • PublicationOpen Access
    Hydrogen peroxide-induced oxidative stress upregulates ght5 gene belonging to hexose transporters in Schizosaccharomyces pombe
    (2019-01-01) Kina, Umit Yasar; Palabiyik, Bedia; KINA, ÜMİT YAŞAR
    Hydrogen peroxide is an agent that triggers oxidative stress. Glucose, which is a source of carbon and energy has a regulatory role in many metabolic processes such as growth rate, fermentation capacity and stress response. Schizosaccharomyces pombe has eight hexose transporters with a different affinity for glucose and/or related monosaccharides. In S. pombe. Ght5 is a glucose transporter with high-affinity. We aimed to investigate the effects of H2O2-induced oxidative stress on hexose transporters using glucose repression-resistant mutant strains (ird5 and ird11) of S. pombe. We analyzed the percentage of glucose consumption in S. pombe wild-type and mutant cells under stressed and non-stressed conditions. Then we compared the expression levels of the genes encoding hexose transporters under the same conditions. We confirmed that the glucose consumption efficiencies of the mutants were slower than the wild-type as in earlier study under non-stressed condition. The percentage of glucose consumption reduced by approximately two-fold in ird11 and wild-type, but not change in ird5, under a stressed condition. There is no difference between cells shape and size of S. pombe strains under stressed and non-stressed conditions. Under stress-induced condition, the expression levels of ght3, ght4 genes in ird11 and wild-type, and ght4, ght6 genes in ird5 decreased, but that of ght5 gene remarkably increased in only wild-type. We suggested that oxidative stress caused by H2O2 leads to upregulation of the ght5 gene in S. pombe.
  • PublicationMetadata only
    Assessing the influence of cycloastragenol on telomere/telomerase system of Arabidopsis thaliana
    (2021-03-01T00:00:00Z) Ceylan, Merve; KINA, ÜMİT YAŞAR; Cakir, Ozgur; Kara, Neslihan Turgut; KINA, ÜMİT YAŞAR
    Cycloastragenol (CAG) is a triterpenoid saponin compound that is synthesized by Astragalus species. CAG has many bioactivity, including telomerase activation, telomere elongation, anti-inflammatory, and anti-oxidative properties. While CAG activates human telomerase, no study has been conducted to investigate its effect on the plant telomere/telomerase system. In this study, in order to test whether CAG has an impact on plants, 1 mu M and 10 mu M CAG were tested on all in vitro grown plant and callus tissues of a model plant Arabidopsis thaliana. Results revealed that CAG caused an increase in the transcript levels of TER1, CTC1, TRB1, POT1a, TAC1, BT2 which act as positive regulators on the telomere/telomerase system together with the TERT gene encoding the catalytic subunit of telomerase especially calli tissues in A.thaliana. While CAG administration resulted in increase in height, and no remarkable effect on callus growth index was observed. Furthermore, no significant change in cell proliferation was observed in plant root cells due to the CAG treatment. The gene expression results suggest that CAG induced transcription of positive regulators of telomere/telomerase system and it might have a role in plants as well as in humans.
  • PublicationMetadata only
    Mitochondrial Spermidine Synthase is Essential for Blood-stage growth of the Malaria Parasite
    (2022-12-01T00:00:00Z) KAMIL, MOHD; KINA, ÜMİT YAŞAR; DEVECİ, GÖZDE; Akyuz, Sevim N.; Yilmaz, Ilknur; ALY, Ahmed Sayed Ibrahım; KAMIL, MOHD; KINA, ÜMİT YAŞAR; DEVECİ, GÖZDE; ALY, AHMED SAYED IBRAHıM
    © 2022Positively-charged polyamines are essential molecules for the replication of eukaryotic cells and are particularly important for the rapid proliferation of parasitic protozoa and cancer cells. Unlike in Trypanosoma brucei, the inhibition of the synthesis of intermediate polyamine Putrescine caused only partial defect in malaria parasite blood-stage growth. In contrast, reducing the intracellular concentrations of Spermidine and Spermine by polyamine analogs caused significant defects in blood-stage growth in Plasmodium yoelii and P. falciparum. However, little is known about the synthesizing enzyme of Spermidine and Spermine in the malaria parasite. Herein, malaria parasite conserved Spermidine Synthase (SpdS) gene was targeted for deletion/complementation analyses by knockout/knock-in constructs in P. yoelii. SpdS was found to be essential for blood-stage growth. Live fluorescence imaging in blood-stages and sporozoites confirmed a specific mitochondrial localization, which is not known for any polyamine-synthesizing enzyme so far. This study identifies SpdS as an excellent drug targeting candidate against the malaria parasite, which is localized to the parasite mitochondrion.
  • PublicationMetadata only
    Otozomal Resesif Nörogelişimsel Bozukluğun Nedeni Olarak Bi-alelik NALCN Varyantı
    (2020-11-30T00:00:00Z) Süsgün, Seda; Göncü, Beyza; Kına, Ümit Yaşar; Hasanoğlu, Sevde; Özgül, Cemil; Çalık, Mustafa; Özbek, Uğur; Uğur İşeri, Sibel Aylin; SÜSGÜN, SEDA; YÜCESAN, EMRAH; GÖNCÜ, BEYZA SERVET; KINA, ÜMİT YAŞAR
  • PublicationMetadata only
    Endoplasmic reticulum localized TMEM33 domain-containing protein is crucial for all life cycle stages of the malaria parasite
    (2024-01-18) Kamil M.; KINA Ü. Y.; Atmaca H. N.; Unal S.; Deveci G.; Burak P.; Aly A. S. I.; KAMIL, MOHD; KINA, ÜMİT YAŞAR; DEVECİ, GÖZDE
    Endoplasmic reticulum (ER) plays a pivotal role in the regulation of stress responses in multiple eukaryotic cells. However, little is known about the effector mechanisms that regulate stress responses in ER of the malaria parasite. Herein, we aimed to identify the importance of a transmembrane protein 33 (TMEM33)-domain-containing protein in life cycle of the rodent malaria parasite Plasmodium berghei. TMEM33 is an ER membrane-resident protein that is involved in regulating stress responses in various eukaryotic cells. A C-terminal tagged TMEM33 was localized in the ER throughout the blood and mosquito stages of development. Targeted deletion of TMEM33 confirmed its importance for asexual blood stages and ookinete development, in addition to its essential role for sporozoite infectivity in the mammalian host. Pilot scale analysis shows that the loss of TMEM33 results in the initiation of ER stress response and induction of autophagy. Our findings conclude an important role of TMEM33 in the development of all life cycle stages of the malaria parasite, which indicates its potential as an antimalarial target.