Person: YÜKSEL MAYDA, PELİN
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Publication Metadata only Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey(2015-05-01T00:00:00Z) Caliskan, Reyhan; Tokman, Hrisi Bahar; Erzin, Yusuf; Saribas, Suat; Yuksel, Pelin; Bolek, Bora Kazim; Sevuk, Ecehan Ozge; Demirci, Mehmet; Yilmazli, Ozge; Akgul, Ozer; Kalayci, Fatma; Cakan, Huseyin; Salih, Barik; Bal, Kadir; Kocazeybek, Bekir; YÜKSEL MAYDA, PELİNIntroduction: Antibiotic resistance is the main factor that affects the efficacy of current therapeutic regimens against Helicobacter pylori. This study aimed to determine the rates of resistance to efficacy clarithromycin, amoxicillin, tetracycline, levofloxacin and metronidazole among H. pylori strains isolated from Turkish patients with dyspepsia. Methods: H. pylori was cultured from corpus and antrum biopsies that were collected from patients with dyspeptic symptoms, and the antimicrobial susceptibility of H. pylori was determined using the E-test (clarithromycin, amoxicillin, tetracycline, metronidazole and levofloxacin) according to the EUCAST breakpoints. Point mutations in the 23S rRNA gene of clarithromycin-resistant strains were investigated using real-time PCR. Results: A total of 98 H. pylori strains were isolated, all of which were susceptible to amoxicillin and tetracycline. Of these strains, 36.7% (36/98) were resistant to clarithromycin, 35.5% (34/98) were resistant to metronidazole, and 29.5% (29/98) were resistant to levofloxacin. Multiple resistance was detected in 19.3% of the isolates. The A2143G and A2144G point mutations in the 23S rRNA-encoding gene were found in all 36 (100%) of the clarithromycin-resistant strains. Additionally, the levofloxacin MIC values increased to 32 mg/L in our H. pylori strains. Finally, among the clarithromycin-resistant strains, 27.2% were resistant to levofloxacin, and 45.4% were resistant to metronidazole. Conclusions: We conclude that treatment failure after clarithromycin-or levofloxacin-based triple therapy is not surprising and that metronidazole is not a reliable agent for the eradication of H. pylori infection in Turkey.Publication Metadata only Problems encountered in conventional HIV 1/2 algorithms: Lack of necessity for immunoblot assays to confirm repeated ELISA reactive results(2018-06-01T00:00:00Z) Yuksel, Pelin; Saribas, Suat; Kuskucu, Mert; Mutcali, Sibel Islak; Kosan, Erdogan; Habip, Zafer; Demirci, Mehmet; Kara, Eda Salihoglu; Birinci, Ilhan; Caliskan, Reyhan; Dinc, HARİKA ÖYKÜ; Midilli, Kenan; Ziver, Tevhide; Kocazeybek, Bekir; YÜKSEL MAYDA, PELİN; DİNÇ, HARİKA ÖYKÜBackground: The use of conventional (serologically based) HIV 1/2 diagnostic algorithms has become controversial in recent years.Publication Metadata only The relationship of Chlamydophila pneumoniae with schizophrenia: The role of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in this relationship(2017-01-01T00:00:00Z) Kalayci, Fatma; Ozdemir, Armagan; Saribas, Suat; Yuksel, Pelin; Ergin, Sevgi; Kuskucu, Ali Mert; Poyraz, Cana Aksoy; Balcioglu, Ibrahim; Alpay, Nihat; Kurt, Aykut; Sezgin, Zeynep; Kocak, Banu Tufan; Icel, Rana Sucu; Can, Gunay; Tokman, Hrisi Bahar; Kocazeybek, Bekir; YÜKSEL MAYDA, PELİNSeveral pathogens have been suspected of playing a role in the pathogenesis of schizophrenia. Chronic inflammation has been proposed to occur as a result of persistent infection caused by Chlamydophila pneumoniae cells that reside in brain endothelial cells for many years. It was recently hypothesized that brain-derived neurotrophic factor ( BDNF) and neurotrophin-3 (NT-3) may play prominent roles in the development of schizophrenia. NT3 and BDNF levels have been suggested to change in response to various manifestations of infection. Therefore, we aimed to elucidate the roles of BDNF and NT3 in the schizophrenia-C. pneumoniae infection relationship. RT-PCR, immunofluorescence and ELISA methods were used. Fifty patients suffering from schizophrenia and 35 healthy individuals were included as the patient group (PG) and the healthy control group (HCG), respectively. We detected persistent infection in 14 of the 50 individuals in the PG and in 1 of the 35 individuals in the HCG. A significant difference was found between the two groups (p 0.05). C. pneumoniae DNA was not detected in any group. A significant difference in NT-3 levels was observed between the groups, with very low levels in the PG (p 0.05). In conclusion, we suggest that NT-3 levels during persistent C. pneumoniaeinfection may play a role in this relationship. (C) 2016 Asociaci on Argentina de Microbiologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND licensePublication Metadata only Evaluation of Adenovirus-36 (Ad-36) antibody seropositivity and adipokine levels in obese children(2017-07-01T00:00:00Z) Kocazeybek, Bekir; Dinc, HARİKA ÖYKÜ; Ergin, Sevgi; Saribas, Suat; Ozcabi, Bahar Taskm; Cizmecigil, Utku; Altan, Eda; Atalik, Keyser; Yuksel, Pelin; Taner, Zeynep; Karakullukcu, Asiye; Sirekbasan, Serhat; Turan, Nuri; Cagatay, Penbe; Imamova, Nergiz; Evliyaoglu, Olcay; Yilmaz, Huseyin; DİNÇ, HARİKA ÖYKÜ; YÜKSEL MAYDA, PELİNAdenovirus 36 (Ad-36) has recently been suggested as a possible contributor to the current obesity epidemic. The aim of this study was to investigate the prevalence of Ad-36 antibodies in obese children, as well as investigate the role of serum leptin and lipid levels in Ad-36-obesity. Seventy-one obese children and 62 non-obese children were included as the patient group (PG), including the healthy control group (HCG), respectively. Simultaneously, Ad-36 antibodies and adipokine levels were assessed with serum neutralization assays (SNA) and ELISA. Ad-36 antibody was detected in 9 patients (12.7%) and 1 patient (1.6%) in both the PG and HCG, respectively, while a significant difference was detected between groups (p < 0.05). Although serum LDL, total cholesterol, triglycerides and leptin levels were detected significantly higher, adiponectin level was detected paradoxically lower in the PG. However, a significant difference was not detected for lipids and leptin levels; adiponectin levels were found to be significantly lower in Ad-36 antibody-positive PG (p < 0.05).Publication Metadata only Serum neopterin and procalcitonin levels in relationship with pediatric burn wound infections(2016-03-01T00:00:00Z) Pilanci, Ozgur; Ergin, Sevgi; Sirekbasan, Serhat; Ersin, Idris; Habip, Zafer; Yuksel, Pelin; Kuvat, Nuray; Aslan, Mustafa; Dinc, Oyku; Saribas, Suat; Kocazeybek, Bekir; YÜKSEL MAYDA, PELİN; DİNÇ, HARİKA ÖYKÜInfection and septic complications in burn patients can be monitored by procalcitonin (PCT) and neopterin plasma values. The aim of the study was to investigate serum neopterin and PCT levels with WBC (white blood cell) and CRP (C-reactive protein) levels in patient group (PG) and healthy control group (HCG) and to investigate the relationship of these markers with burn wound infections (BWI). As the PG, 23 patients between 0-12 ages and up to 30% Total Body Surface Area (TBSA) burned and 15 HCG were included. PCT, neopterin, WBC, and CRP results on the first, the seventh, the fourteenth and the 21st day have been compared. During the follow-up period, 11 patients with BWI and 12 patients without BWI were classified as infected and non-infected patients, respectively. PCT and neopterin levels were detected higher in patients with BWI but no significant difference were present. Also, PCT and neopterin levels within the first 24 hours following the burn were detected higher in PG than HCG. CRP and WBC levels were detected high due to burn trauma. PCT and neopterin levels were increased in patients with BWI. PCT levels were increased during the pre-infectious period, while neopterin levels increased during the post-infectious period.Publication Metadata only The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains(2015-07-01T00:00:00Z) Caliskan, Reyhan; Yazgan, Ayca Sayi; Tokman, Hrisi Bahar; Sofyali, Emre; Erzin, Yusuf Ziya; Akgul, Ozer; Kurt, Aykut; Kalayci, Fatma; Ziver, Tevhide; Yuksel, Pelin; Bal, Kadir; Kocazeybek, Bekir; YÜKSEL MAYDA, PELİNBackground/Aims: Helicobacter pylori (H. pylori) is a microaerophilic bacterium related with peptic ulcer and gastric cancer. Its virulence factors include cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) proteins. Cytokine release inducted by H. pylori colonization has an important role in pathogenesis of H. pylori. The severity of gastric pathologies depends on the H. pylori genotypes found in different geographical regions. We aimed to determine the relationship between different H. pylori genotypes and their effects on the cytokine release levels.Publication Metadata only The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1(2017-09-01T00:00:00Z) Uysal, Hayriye Kirkoyun; Adas, Gokhan Tolga; Atalik, Kevser; Altiparmak, Semih; Akgul, Ozer; Saribas, Suat; Gurcan, Mert; Yuksel, Pelin; Yildirmak, Taner; Kocazeybek, Bekir; Ziver, Tevhide; Oner, Yasar Ali; YÜKSEL MAYDA, PELİNOpportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS). We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl-Neelsen (ZN). The primers and probes used for PCR were Heat shock protein 70 for C. cayetanensis and the oocysts wall protein for Cryptosporidium spp.. Cyclospora and Cryptosporidium spp. oocysts were detected in one and two patients, respectively, by staining, whereas we detected C. cayetanensis in three patients out of 115 (2.6%) by PCR, and Cryptosporidium spp. in a further three patients (2.6%). C. cayetensis was detected in patients with CD4 counts of 64 cells/mu m, 182 cells/mu m and 287 cells/mu m, respectively. Cryptosporidium spp. was detected in patients with CD4 counts of 176 cells/mu m, 241 cells/mu m and 669 cells/mu m. As conclusion, PCR method is faster and more sensitive than microscopic methods and to screen intestinal pathogens routinely in patients infected with HIV should not be neglected in developing countries like Turkey.Publication Metadata only Neopterin and soluble CD14 (sCD14) levels as immunoactivation markers in aHBc-only cases(2017-08-01) Dinc, Harika Oyku; SOHRABİ, Pari; Habip, Zafer; Saribas, Suat; Vatan, Asli; Altun, Yagiz M.; ZİVER, Tevhide; Caliskan, Reyhan; Demirci, Mehmet; Uysal, Omer; Yuksel, Pelin; Kocazeybek, Bekir; DİNÇ, HARİKA ÖYKÜ; UYSAL, ÖMER; YÜKSEL MAYDA, PELİNAim: Isolated hepatitis B core antibodies (aHBc)-only pattern complicates the diagnosis of HBV infections. We evaluated neopterin and sCD14 levels in HBV infections. Methods: aHBc-only (n: 102), healthy control (healthy control group [HCG], n: 100), and chronic hepatitis (CHB) groups (n: 70) were investigated. Competitive and sandwich ELISA were used. Results: The mean neopterin levels were significantly lower in the aHBc-only group than those in the CHB group (p = 0.0001). No significant difference was found between the aHBc-only group and the HCG (p = 0.854). The mean sCD14 levels were lower in the aHBc-only group than those in the CHB group (p = 0.0001), but no significant difference was found between the aHBc-only group and HCG (p = 0.402). No significant difference was detected between aHBc-only and HCG for mean sCD14 (p = 0.402) and neopterin levels (p = 0.854). Conclusion: These two biomarkers are not useful for diagnosing the aHBc only pattern.