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TEKKELİ, ŞERİFE EVRİM

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ŞERİFE EVRİM
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TEKKELİ
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2010 - 2019372020 - 202217
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    PublicationOpen Access
    An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization
    (2017-03-01) Tekkeli, S. E. Kepekci; GAZIOGLU, IŞIL; KIZILTAS, M. V.; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL
    A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.
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    ANALYTICAL METHODS FOR THE DETERMINATION OF POLYBROMINATED DIPHENYL ETHERS IN HUMAN MILK
    (2020-03-01T00:00:00Z) ASRAM SAĞIROĞLU, ALİ; Onal, Cem; DİNCEL, DEMET; Onal, Armagan; ASRAM SAĞIROĞLU, ALİ; TEKKELİ, ŞERİFE EVRİM; DİNCEL, DEMET
    Polybrominated diphenyl ethers (PBDEs), which are used as flame retardants, are widely used additives so many different kind of materials that are consumed by public. Current researches reveal a significant increase in the levels of PBDEs in human biological fluids all around the world. According to International Agency for Research on Cancer (IARC), PBDEs are declarated as carcinogenic agents and additionally a considerable risks have been shown in animal studies such as liver damage, alteration of thyroid hormone levels, neurotoxicity and hazardous effects on development of fetus. Due to the simplicity of human exposure routes such as ingestion, inhalation or dermal contact, it gains high importance to quantify PBDEs in biological fluids. The exposure to PBDEs is critic aspecially during pregnancy, fetus development and infancy. Excretion of PBDEs in human milk is the focus area for researchers in recent years.
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    Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography
    (2010-01-20T00:00:00Z) Öztekin, Nevin; Baskan, Selda; Kepekci, S. Evrim; Erim, F. Bedia; Topcu, Guelacti; TEKKELİ, ŞERİFE EVRİM; TOPÇU, GÜLAÇTI
    In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia Species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 mu g/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts. (C) 2009 Elsevier B.V. All rights reserved.
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    SIMULTANEOUS DETERMINATION OF FEBUXOSTAT AND MONTELUKAST IN HUMAN PLASMA BY USING FABRIC PHASE SORPTIVE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
    (2021-09-17T00:00:00Z) GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM; Kabir, Abuzar; Aslan, Ceylin; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM
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    Spectrofluorimetric Methods for the Determination of Gemifloxacin in Tablets and Spiked Plasma Samples
    (2011-05-01) Tekkeli, Serife Evrim Kepekci; Onal, Armagan; TEKKELİ, ŞERİFE EVRİM
    Two new, sensitive and selective spectrofluorimetric methods have been developed for the determination of gemifloxacin (GFX) in tablets and spiked plasma samples. Gemifloxacin, as a primary amine compound, reacts with 7-chloro-4-nitrobenzofurazon (NBD-Cl) (for method A) and fluorescamine (for method B) which are a highly sensitive fluorogenic reagents used in many investigations. For method A, the reaction product was measured spectrofluorimetrically at 516 nm with excitation at 451 nm. The reaction proceeded quantitatively at pH 8.5, 80 A degrees C in 7 min. For method B, the method was based on the reaction between GFX and fluorescamine in borate buffer solution of pH 8.5 to give highly fluorescent derivatives that were measured at 481 nm using an excitation wavelength of 351 nm. The fluorescence intensity was directly proportional to the concentration over the range 40-200 ng mL(-1) and 100-1,200 ng mL(-1) for method A and B, respectively. Successful applications of the developed methods, for the drug determination in pharmaceutical preparations and spiked plasma samples, were performed.
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    PublicationOpen Access
    Effects of prebiotic oligofructose-enriched inulin on gut-derived uremic toxins and disease progression in rats with adenine-induced chronic kidney disease
    (2021-10-06T00:00:00Z) Cetinkaya, M. Alper; Kepekci-Tekkeli, Şerife Evrim; Kul, Oguz; Samur, Gulhan; TEKKELİ, ŞERİFE EVRİM
    Recent studies suggest that dysbiosis in chronic kidney disease (CKD) increases gut-derived uremic toxins (GDUT) generation, leads to systemic inflammation, reactive oxygen species generation, and poor prognosis. This study aimed to investigate the effect of oligofructose-enriched inulin supplementation on GDUT levels, inflammatory and antioxidant parameters, renal damage, and intestinal barrier function in adenine-induced CKD rats. Male Sprague-Dawley rats were divided into control group (CTL, n = 12) fed with standard diet; and CKD group (n = 16) given adenine (200 mg/kg/day) by oral gavage for 3-weeks to induce CKD. At the 4th week, CKD rats were subdivided into prebiotic supplementation (5g/kg/day) for four consecutive weeks (CKD-Pre, n = 8). Also, the control group was subdivided into two subgroups; prebiotic supplemented (CTL-Pre, n = 6) and non-supplemented group (CTL, n = 6). Results showed that prebiotic oligofructose-enriched inulin supplementation did not significantly reduce serum indoxyl sulfate (IS) but did significantly reduce serum p-Cresyl sulfate (PCS) (p = 0.002) in CKD rats. Prebiotic supplementation also reduced serum urea (p = 0.008) and interleukin (IL)-6 levels (p = 0.001), ameliorated renal injury, and enhanced antioxidant enzyme activity of glutathione peroxidase (GPx) (p = 0.002) and superoxide dismutase (SOD) (p = 0.001) in renal tissues of CKD rats. No significant changes were observed in colonic epithelial tight junction proteins claudin-1 and occludin in the CKD-Pre group. In adenine-induced CKD rats, oligofructose-enriched inulin supplementation resulted in a reduction in serum urea and PCS levels, enhancement of the antioxidant activity in the renal tissues, and retardation of the disease progression.
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    Çocuklarda Dikkat Eksikliği Hiperaktivite Bozukluğu Durumunda Kullanılan İlaçlar, Gıda Takviyeleri ve Fitofarmasötiklerin İncelenmesi
    (2021-11-17T00:00:00Z) Tekkeli, Şerife Evrim; Özbek, Emirhan; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİM
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    High performance liquid chromatographic analysis of lercanidipine in human breast milk
    (2019-01-01) Tekkeli, Evrim Kepekci; GAZİOĞLU, IŞIL; TIRIS, GİZEM; Onal, Cem; TEKKELİ, ŞERİFE EVRİM; GAZİOĞLU, IŞIL; TIRIS, GİZEM
    A simple, rapid, precise and accurate isocratic reversed phase HPLC method was developed and validated for the determination of lercanidipine hydrochloride in pharmaceutical tablets and spiked human breast milk. The chromatographic separation was achieved on C18 (250x4.6 mmx5 mu m) column using a mobile phase consisting of acetonitrile and phospate buffer (pH=4) (55:45, v/v) at a flow rate of 1.1 mL/min and UV detection at 237 nm. The linearity of the proposed method was investigated in the range of 1.0-40 mu g/mL (r(2)=0.9990). The method was validated in terms of accuracy, precision, reproducibility, specificity, robustness, and detection and quantification limits, in accordance with ICH guidelines. The proposed method is found as suitable for routine quantification of lercanidipine in human breast milk.
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    Spectrofluorimetric determination of tobramycin in human serum and pharmaceutical preparations by derivatization with fluorescamine
    (2014-02-01) Tekkeli, Serife Evrim Kepekci; Onal, Armagan; Sagirli, Olcay; TEKKELİ, ŞERİFE EVRİM
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    An HPLC Method for the Determination of Saxagliptin in Human Plasma with Fluorescence Detection
    (2013-12-01) Tekkeli, Şerife Evrim; Kızıltas, M. V.; Dincel, Demet; TEKKELİ, ŞERİFE EVRİM; DİNCEL, DEMET