Person: GÜNEŞ BAYIR, AYŞE
6 results
Search Results
Now showing 1 - 6 of 6
Publication Metadata only Cytotoxic, genotoxic and apoptotic activities of carvacrol from Thymus vulgaris on the human adenocarcinoma gastric cells(2015-08-01) GUNES-BAYIR, Ayse; Kiziltan, HURİYE ŞENAY; Guler, ERAY METİN; KARAKAS, Ersin; Kocyigit, ABDÜRRAHİM; GÜNEŞ BAYIR, AYŞE; KIZILTAN, HURİYE ŞENAY; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only In vitro effects of two major phenolic compounds from the family Lamiaceae plants on the human gastric carcinoma cells(2018-08-01) GÜNES-BAYIR, Ayse; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİNPublication Metadata only Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.(2018-11-01) Günes-Bayir, AYŞE; Kocyigit, ABDÜRRAHİM; Güler, ERAY METİN; Bilgin, MEHMET GÜLTEKİN; Ergün, İLYAS SAMET; DADAK, A; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; BİLGİN, MEHMET GÜLTEKİN; ERGÜN, İLYAS SAMETPublication Metadata only Anti-Inflammatory and Antioxidant Effects of Carvacrol on N-Methyl-N′-Nitro-N-Nitrosoguanidine (MNNG) Induced Gastric Carcinogenesis in Wistar Rats(2022-07-01T00:00:00Z) Güneş Bayır, Ayşe; Guler, Eray Metin; Bilgin, Mehmet Gultekin; Ergun, Ilyas Samet; Kocyigit, Abdurrahim; Dadak, Agnes; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİMCarvacrol is a dietary polyphenol from Lamiaceae plants that has been shown to possess a wide range of biological activities including antioxidant and antitumor effects. This study aimed to investigate its anti-inflammatory and antioxidant effects on N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) induced gastric carcinogenesis in Wistar rats. Forty-nine rats were randomly assigned to four treatment and three control groups. Over 60 days, MNNG (200 mg/kg BW) was orally applied to animals of groups 1–5 while the rats in groups 2–5 also received different doses of carvacrol (10, 25, 50, and 100 mg/kg BW, respectively) until the end of the experiment. Group 6 rats were treated with 100 mg/kg BW carvacrol and no MNNG whereas group 7 was the control group without any treatment. After the euthanasia of all rats, the inflammatory cytokines and oxidative stress parameters were assessed in the blood and tissues. The expression of caspase 9, Bax, and Bcl-2 proteins in the stomach tissues were investigated through histopathological examinations. Statistically significant differences were observed in the body weight, oxidative stress, and inflammation parameters of groups 1 to 6 compared to group 7 (p ≤ 0.001). Animals in MNNG groups 2 and 3 treated with the low dose carvacrol (10 and 25 mg/kg BW) showed significantly reduced oxidative stress, inflammation, and apoptotic effect compared to animals of the MNNG groups receiving increased doses of carvacrol (50 and 100 mg/kg BW) or no carvacrol. Rats exposed to MNNG exhibited gastric cancer cells in several areas. In the MNNG group receiving 100 mg/kg BW carvacrol, the inflammatory cell infiltration was observed in gastric mucosal and submucosal areas whereas MNNG rats supplemented with 10 and 25 mg/kg BW carvacrol showed no pathological alterations of the gastric cells. The results of this study indicate that significant antioxidant and anti-inflammatory effects induced by carvacrol at doses of 10 and 25 mg/kg BW interfered with gastric carcinogenesis induced by MNNG in Wistar rats as well as provide hepatoprotection. However, high doses of carvacrol (50 and 100 mg/kg BW) increased oxidative stress, inflammation, and apoptosis.Publication Metadata only Effects of natural phenolic compound carvacrol on the human gastric adenocarcinoma (AGS) cells in vitro(2017-06-01) GUNES-BAYIR, Ayse; KIZILTAN, Huriye S.; Kocyigit, ABDÜRRAHİM; Guler, Eray M.; KARATAS, Ersin; Toprak, ALİ; GÜNEŞ BAYIR, AYŞE; KIZILTAN, HURİYE ŞENAY; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; TOPRAK, ALİGastric cancer (GC) is the most common cause of morbidity and mortality because of cancer. Medicinal plants containing polyphenolic compounds have gained importance in anticancer treatment. In this context, carvacrol is a main component of many plants in the family Lamiaceae that are frequently used in folk medicine and a good candidate to investigate for GC treatment. The present study aimed to explore the cytotoxic, genotoxic, apoptotic, and reactive oxygen species (ROS)-generating effects of carvacrol on gastric adenocarcinoma in vitro. For these purposes, human gastric adenocarcinoma (AGS) cells were used and analyzed after 24h of exposure to carvacrol with different concentrations. The cytotoxicity, ROS generation, glutathione (GSH) level, and genotoxicity were investigated by the ATP cell viability assay, 2,7-dichlorodihydrofluorescein-diacetate assay, GSH/GSSG-Glo assay, and comet assay, respectively. Apoptosis induction was detected by acridine orange/ethidium bromide staining and western blotting at below the half-maximal growth inhibitory concentration value. Carvacrol showed cytotoxic, genotoxic, apoptotic, ROS generating, and GSH-reducing effects on AGS cells in a dose-dependent manner. There was a close negative relationship between cell viability and ROS level. Carvacrol inhibited the proliferation of AGS cells, suggesting that it could be a novel and strong anticancer agent against the human gastric adenocarcinoma. These results support the interest of natural diet components in the development of therapeutic products for diseases.Publication Metadata only Effects of Thymol, a Natural Phenolic Compound, on Human Gastric Adenocarcinoma Cells In Vitro.(2019-03-01) GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; KIZILTAN, HÜRİYE ŞENAY; GÜNEŞ BAYIR, AYŞE; KOÇYİĞİT, ABDÜRRAHİM; GÜLER, ERAY METİN; KIZILTAN, HURİYE ŞENAY