Person: EROL, EBRU
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Publication Metadata only Chemical constituents of essential oil of endemic Rhanterium suaveolens Desf. growing in Algerian Sahara with antibiofilm, antioxidant and anticholinesterase activities(2016-01-01T00:00:00Z) Chemsa, Ahmed Elkhalifa; Erol, EBRU; Ozturk, Mehmet; Zellagui, Amar; Ozgur, Ceylan; Gherraf, Noureddine; Duru, Mehmet Emin; EROL, EBRUTwenty compounds were detected in the essential oil of Rhanterium suaveolens representing 98.01% of the total oil content. Perillaldehyde (45.79%), caryophyllene oxide (24.82%) and -cadinol (5.61%) were identified as the main constituents. In -carotene-linoleic acid assay, both the oil and the methanol extract exhibited good lipid peroxidation inhibition activity, with IC50 values of 17.97 +/- 5.40 and 11.55 +/- 3.39g/mL, respectively. In DPPH and CUPRAC assays, however, the methanol extract exhibited a good antioxidant activity. The highest antibiofilm activity has been found 50.30% against Staphylococcus epidermidis (MU 30) at 20g/mL for essential oil and 58.34% against Micrococcus luteus (NRRL B-4375) at 25mg/mL concentration for methanol extract. The in vitro anticholinesterase activity of methanol extract showed a moderate acetylcholinesterase inhibitory (IC50=168.76 +/- 0.62g/mL) and good butyrylcholinesterase inhibitory (IC50=54.79 +/- 1.89g/mL) activities. The essential oil was inactive against both enzymes.Publication Metadata only Antibiofilm formation, antioxidant and anticholinesterase activities of essential oil and methanol extract of Marrubium deserti de Noé(2016-01-01T00:00:00Z) Chemsa, Ahmed Elkhalifa; Zellagui, Amar; Öztürk, Mehmet; EROL, EBRU; Ceylan, Ozgür; Duru, Mehmet Emin; Gherraf, Noureddine; EROL, EBRUThe essential oil obtained from the aerial parts of Marrubium deserti de Noé. (Lamiaceae), growing in the North fringe of the Algerian Sahara, was analyzed by GC-MS. Thirty-eight compounds were identified, representing 99.70% of the total oils. The GC-MS analysis revealed the presence of tetracosane, germacrene D, Δ-cadinene, a-cadinol and t-cadinol as the main constituents, representing 31.11%, 7.91%, 6.52%, 6.26% and 5.81%, respectively. Minimum inhibitory concentrations (MICs) of essential oil and methanol extract were calculated by microtitre broth dilution method, and antibiofilm effects by microplate biofilm assay. The highest antibiofilm activity was found to be 69.31% against Micrococcus luteus NRRL B-4375 at 25 mg/mL for methanol extract and 36.62% against Candida albicans ATCC 10239 at 25 μL/mL concentration for essential oil. The antioxidant activity was determined using three complementary tests namely: β-carotene-linoleic acid, DPPHfree radical scavenging, and CUPRAC assays. In β-carotene-linoleic acid assay, both the oil and the extract exhibited good lipid peroxidation inhibition activity, demonstrating 76.81 ± 0.59 and 86.33 ± 0.27% at 200 μg/mL concentration, respectively. In DPPH and CUPRAC assays, however, the methanol extract exhibited high antioxidant activity; however, the essential oil showed weak activity. The in vitro anticholinesterase activity, was carried out against acetylcholinesterase and butyrylcholinesterase enzymes spectrophotometrically using Elman method. Methanol extract showed weak acetylcholinesterase and butyrylcholinesterase inhibitory activities, while the essential oil was inactive against both enzymes.Publication Metadata only Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of essential oil and methanolic extract of Anthemis stiparum subsp sabulicola (Pomel) Oberpr(2018-06-01T00:00:00Z) Chemsa, Ahmed Elkhalifa; Zellagui, Amar; Ozturk, Mehmet; Erol, EBRU; Ceylan, Ozgur; Duru, Mehmet Emin; Lahouel, Mesbah; EROL, EBRUAnthemis species are traditionally used to treat infectious and inflammatory processes, among others clinical disturbances. In the current study, the chemical composition, the total phenolic and flavonoid contents, the antioxidant, anticholinesterase, antimicrobial, and antibiofilm activities of Anthemis stiparum subsp. sabulicola aerial parts methanolic extract (As-ME) and essential oil (As-EO) were investigated. The chemical composition of As-EO was established by GC-MS and GC-FID. Total phenolic and flavonoid contents of As-ME were spectrophotometrically determined. Diphenyl-1-picrylhydrazyl (DPPH center dot) radical scavenging, cupric reducing antioxidant capacity (CUPRAC) and beta-carotene bleaching assays were applied to evaluate the antioxidant potential. The anticholinesterase activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were carried out spectrophotometrically. The antimicrobial activity was assessed by Minimal Inhibitory Concentration (MIC) using broth microdilution method against 7 ATCC(center dot) bacterial and one ATCC(center dot) yeast reference strains. The antibiofilm effect was determined quantifying the percentage of adhesion inhibition. GC-MS and GC-FID identified 72 compounds (99.02%), being As-EO predominantly constituted by germacrene D (11.13%), t-cadinol (11.01%), camphor (6.73%), spathulenol (6.50%) and isoamyl salicylate (6.45%). The total phenolic and flavonoid contents of As-ME were 13.6 +/- 0.03 and 5.9 +/- 0.04 pyrocatechol equivalents and quercetin equivalents, respectively. In beta-carotene-linoleic acid assay, As-ME showed the best lipid peroxidation inhibition activity with an IC50 = 9.96 mu g/mL followed by As-EO with an IC50 = 619.98 mu g/mL. In contrast, in DPPH assay, As-ME and As-EO showed moderate to low activity with an IC50 = 92.69 mu g/mL for As-ME and 917.69 mu g/mL for As-EO. While in CUPRAC assay, As-EO and As-ME indicated a less to moderate reducing activity. As-ME inhibited AChE (IC50 = 490.46 mu g/mL) and BChE (IC50 = 142.07 mu g/mL), while As-EO was inactive against AChE and revealed a discreet inhibitory action against BChE (IC50 = 212.14 mu g/mL). As-ME displayed better antimicrobial activity than As-EO, being active against Staphylococcus aureus (ATCC(center dot) 25923) and Bacillus subtilis (ATCC(center dot) 6633), with MIC of 1.56 mg/mL. An expressive fungal adhesion inhibition (80.02%) on Candida albicans (ATCC(center dot) 10239) was detected with As-ME at 6.25 mg/mL. These results showed that A. stiparum subsp. sabulicola is a natural source of active compounds with antibiotic and antibiofilm effects against S. aureus and B. subtilis, and C. albicans, respectively, and also presents antioxidant and anticholinesterase properties.