Person:
AKKAN, AHMET GÖKHAN

Profile Picture
Status
Organizational Units
OrgUnit Logo
Organizational Unit
Job Title
First Name
AHMET GÖKHAN
Last Name
AKKAN
Name
Email Address
Birth Date

Filters

2020 - 20227
Reset filters

Settings

Start date: 2020 × End date: 2022 × Type: Article × Item Type: Publication ×

Search Results

Now showing 1 - 7 of 7
  • No Thumbnail Available
    PublicationMetadata only
    The Anti-Inflammatory Effects of Anacardic Acid on a TNF-alpha Induced Human Saphenous Vein Endothelial Cell Culture Model
    (2020-01-01T00:00:00Z) DURSUN, Erdinç; Onal, Burak; Ozen, Deniz; Demir, Bulent; Ak, Duygu Gezen; Demir, Caner; Akkan, Ahmet Gökhan; Ozyazgan, Sibel; AKKAN, AHMET GÖKHAN
    Background and Objective: Coronary bypass operations are commonly performed for the treatment of ischemic heart diseases. Coronary artery bypass surgery with autologous human saphenous vein maintains its importance as a commonly used therapy for advanced atherosclerosis. Vascular inflammation-related intimal hyperplasia and atherosclerotic progress have major roles in the pathogenesis of saphenous vein graft disease.
  • Thumbnail Image
    PublicationOpen Access
    The Prevalence of Potential Drug-Drug Interactions in CKD-A Retrospective Observational Study of Cerrahpasa Nephrology Unit
    (2022-02-01T00:00:00Z) BUKHARI, ANDLEEB; Sonmez, Ikbal; Kose, Cagla; OKTAN, BURHANEDDİN; Alagoz, Selma; Sonmez, Haktan; Hussain, Adil; AKKAN, Ahmet Gökhan; AKKAN, AHMET GÖKHAN
    Background and Objectives: Chronic kidney disease (CKD) is usually linked with polypharmacy and patients are invariably at risk of complex medication regimens. The present study was designed to estimate the potential drug-drug interactions (pDDIs) through the prescription patterns provided to patients of the Nephrology Transplant Unit of Cerrahpasa Medical Faculty patients. Materials and Methods: 96 patients were included in the study. pDDIs among every combination of the prescribed drug were analyzed using the Thomson Reuters Micromedex. Results: We found 149 pDDIs making 2.16 interactions per prescription with incidence rates of 69.7%. Approximately 4.1% of interactions were of significant severity, 75.1% moderate severity, and 20.8% were classified as minor pDDIs. The most frequent interactions were found between iron and aluminum, calcium or magnesium-containing products (21.37%), calcium channel blockers and beta-blockers (8.96%); and aspirin and aluminum, calcium, or magnesium-containing products (7.58%). We identified 42 drug pairs with probability of clinical significance. The most commonly reported clinical outcomes of the pDDIs were hypo- or hypertension (39.24%), decreased drug efficacy (24.05%), and arrhythmia (9.49%). Aluminum, calcium, or magnesium-containing drug products (33.10%) constituted the primary class of drugs involved in interactions. Conclusions: This study showed pharmacodynamics (49%), pharmacokinetics (42.94%) interactions, polypharmacy and gender as determinant of pDDIs. A comprehensive multicenter research is required to decrease the morbidity and ease the state burden.
  • No Thumbnail Available
    PublicationMetadata only
    Effect of Three PDEIs on Neuroprotective and Autophagy Proteins in vitro AD Model
    (2021-01-01T00:00:00Z) Saygisever-Faikoglu, Kubra; Faikoglu, Gokhan; Celik, Hande; Ugur, Sedat Askin; AKKAN, Ahmet Gökhan; Kelicen-Ugur, Pelin; Ozyazgan, Sibel; AKKAN, AHMET GÖKHAN
    Background and Objective: The effects of PDEIs on neuroprotective SIRT1 and SESN2, on the autophagy-related proteins, are unknown but neuroprotective enzymes (sirtuins and sestrins) with autophagy genes are involved in the pathogenesis of Alzheimer-s disease. In this study, we aimed to elucidate the effect of three PDE Inhibitors (PDEIs) as autophagy enhancers and provide insights into their neuroprotective effects. Materials and Methods: HT-22 cells were exposed to A$ 25-35 with or without PDEIs for 32 hrs. qRT-PCR was performed for SIRT1, SESN2, ATG5 and BECN1 genes. Western blot analysis was performed for neuroprotective SIRT1, SESN2 proteins and autophagy proteins such as p-mTOR/mTOR, p-AMPK/AMPK and LC3. Results: A$ 25-35 exposure decreased SIRT1, ATG5 and BECN1 expression, while PDEIs prevented these genes from the A$ 25-35 induced decrease. Increased SESN2 gene expression by A$ 25-35 exposure was decreased by PDEIs treatment. Western blot experiment has also shown that SIRT1, p-AMPK and autophagy marker LC3II were decreased, whereas SESN2 and p-mTOR were elevated in the A$ 25-35 exposed HT-22 cells. Co administration of three PDEIs with A$ 25-35 recovered SIRT1, p-AMPK and LC3II decline and compensated SESN2 increase by elevating SIRT1, p-AMPK and LC3II expression and decreasing p-mTOR expression. Conclusion: The present study revealed the significant neuroprotective and autophagy stimulating potential of three PDEIs in A$-induced in vitro AD model. SIRT1 is a novel candidate for determining new, safe and effective treatment strategies and PDEI-mediated SIRT1 increase may advocate autophagy activation through different autophagy components.
  • No Thumbnail Available
    PublicationMetadata only
    The Effect of Orphenadrine on Rewarding Property of Morphine-Induced Conditioned Place Preference
    (2020-05-01T00:00:00Z) Köse, Çağla; Bukharı, Andleeb; Akkan, Ahmet Gökhan; Özyazgan, Sibel; AKKAN, AHMET GÖKHAN
  • No Thumbnail Available
    PublicationMetadata only
    Vascular responses disrupted by fructose-induced hyperinsulinemia improved with delta-9- tetrahydrocannabinol
    (2021-02-01T00:00:00Z) Todurga Seven, Zeynep Gizem; Bolkent, Sema; Köse, Çağla; Çoşkun, Zeynep Mine; Gökdemir, Selim; Akkan, Ahmet Gökhan; Özyazgan, Sibel; AKKAN, AHMET GÖKHAN
    : In recent years, cannabinoids have been shown to have beneficial effects on diabetic vascular complica tions. Vascular complications due to fructose-induced hyperinsulinemia (HI) and diabetic vascular complications have similar mechanisms. The aim of this experimental study was to observe whether the cannabinoid agonist delta-9-te trahydrocannabinol (THC) has an ameliorating effect on fructose-induced HI and vascular responses in the aortic ring of rats with HI. Methods: A total of 24 rats were categorized into 4 groups: control (standard food pellets and water), HI (water contain ing 10% fructose provided for 12 weeks), THC (1.5 mg/kg/day intraperitoneal administration for 4 weeks), and THC+HI. Body weight was measured again on the last day of the study and the serum insulin level was measured with an en zyme-linked immunosorbent assay. The acetylcholine (ACh) maximum relaxant effect in aortic rings pre-contracted with noradrenaline (NA) was evaluated. Results: The body weight of THC and THC+HI groups was lower compared with that of the controls (p<0.01). Increased insulin level as a result of fructose consumption decreased with THC administration (p<0.01) while the glucose level increased in all other groups compared with the control group (p<0.01, p<0.05). The NA Emax value decreased in the group receiving THC treatment (p<0.01). The increased ACh pD2 value in the HI groups also decreased in the THC treatment group (p<0.0001). The decreased maximum inhibition value in the HI group increased significantly with THC administration (p<0.001). Conclusion: THC demonstrated beneficial effects on fructose-induced HI. THC improved ACh-induced endothelial dependent relaxation in HI rat aortic rings. Keywords: Endothelial-dependent relaxation, hyperinsulinemia, tetrahydrocannabinol, vascular response
  • No Thumbnail Available
    PublicationMetadata only
    Investigation of the pharmacological potential of myricetin on alcohol addiction in mice
    (2022-01-01T00:00:00Z) Yunusoglu, Oruc; BUKHARI, ANDLEEB; Turel, Canan Akunal; Demirkol, Muhammed Hamdi; Berköz, Mehmet; AKKAN, Ahmet Gökhan; AKKAN, AHMET GÖKHAN
    Alcohol addiction is one of the leading causes which is associated with morbidity and mortality with outcomes in high healthcare and economic costs. Myricetin is a flavonoid that demonstrates therapeutic actions in many central nervous system diseases. In the current study, the conditioned place preference (CPP) tests were performed W examine the effects of myricetin on ethanol reward. During conditioning, intraperitoneal (i.p) administration of ethanol (2 g/kg) and serum physiologic were given on alternate days for 8 days. In order to evaluate the effect of myricetin on the development of alcohol addiction, myricetin was injected into mice 30 minutes before ethanol administration. Subsequently, a daily myricetin injection was performed to evaluate the effect of myricetin on the extinction of alcohol addiction. Finally, ethanol was administered 900 seconds after different dose myricetin administration, and reinstatement was evaluated immediately thereafter. Systemic ethanol (2 g/kg, i.p) administration significantly produced CPP. Myricetin (5 and 10 mg/kg, i.p) attenuated the development of ethanol addiction (p < 0.05). Systemic myricetin injections immediately after each extinction period precipitated extinction and decreased reinstatement (10 mg/kg, i.p, p < 0.05, respectively). Ethanol alone and in combination with myricetin did not change locomotor activity and motor coordination. As a result, it can be suggested that myricetin is effective in attenuating the rewarding effect of alcohol in mice and can be used for the adjunctive therapy for alcohol addiction. In addition, it will be appropriate to conduct mechanistic experimental studies regarding these results in the future.
  • No Thumbnail Available
    PublicationMetadata only
    TNF-α ile Oluşturulan in Vitro İnsan Safen ven Ateroskleroz Modelinde Atorvastatin ve Pitavastatinin Matriks Metalloproteinaz 2 Üzerine Etkilerinin Karşılaştırılması
    (2021-08-01T00:00:00Z) Özen, Deniz; Önal, Burak; Gezen Ak, Duygu; Dursun, Erdinç; Akkan, Ahmet Gökhan; AKKAN, AHMET GÖKHAN
    Giriş: Ateroskleroz, arteryal duvarda kolesterol içeren düşük dansiteli lipoprotein parçacıklarının birikmesi ile tetiklenen farklı tip hücreler ve ekstraselüler matriks birikiminin kalınlaşmaya sebep olduğu kronik inflamatuvar bir hastalıktır. Aterosklerotik süreçte subendotelyal matriks modifikasyonuna dahil olan başta matriks metalloproteinazları (MMP) olmak üzere birçok yapısal proteinlerin ve enzimlerin sentezi ile salınımında artışlar görülür. Patolojik vasküler dokularda özellikle MMP-2, -3 ve -9 ekspresyonunda ve bu enzimlerin proteolitik aktivitelerinde artış meydana gelir. Statinler, 3-hidroksi-3-metilglutaril-koenzim A redüktaz enzimini inhibe ederek kolesterol sentezini azaltan lipid düşürücü ajanlardır, ateroskleroz ve vasküler endotelyal hasara karşı koruyucu etkilere sahiptir. Statinler, direkt olarak vasküler ve immün hücrelerinin inflamatuvar fonksiyonlarını etkileyerek pleiotropik anti-inflamatuvar etki gösterirler. Gereç ve Yöntemler: Çalışmamızda, insan safen ven endotelyal hücrelerinde (HSaVEC) tümör nekroz faktörü alfa (TNF-α) ile uyararak oluşturduğumuz aterosklerozun inflamatuvar süreci modelinde, koroner bypass cerrahisi sonrası sıklıkla reçetelenen statinlerden atorvastatin ve pitavastatinin anti-inflamatuvar etkilerini karşılaştırdık. HSaVEC’lere 10 ve 50 µM atorvastatin ve 1 ve 2 µM pitavastatin uygulamalarının ardından, TNF-α ile inflamasyon oluşturduk, laktat dehidrogenazı (LDH) spektrofotometrik olarak ölçerek sitotoksisiteleri belirledik. MMP-2 ekspresyon seviyelerini qRT-PCR ile tespit ettik. Bulgular: DMSO grubu ile karşılaştırıldığında, her iki ilaç uygulamasının yapıldığı gruplardaki LDH düzeylerinin daha düşük olduğunu belirledik. TNF- uygulaması sonucunda, MMP-2 ekspresyon seviyesinin kontrol grubuna göre anlamlı olarak azaldığını gözlemledik (p<0.05). Buna karşın farklı dozlarda atorvastatin ve pitavastatin uygulamasının ardından TNF- ile uyarılan hücrelerde MMP-2 ekspresyon seviyesinin anlamlı düzeyde arttığını tespit ettik (p<0.05). Tartışma: Düşük sitotoksik etki gösteren bu iki statin uygulamasının, in vitro ateroskleroz modelinde MMP-2 ekspresyonu üzerindeki etkileri saptanarak, inflamasyonda düzenleyici etkilere sahip oldukları gözlemlenmiştir. Anahtar Kelimeler: Ateroskleroz, safen ven, statin