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TOPÇU, GÜLAÇTI

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Start date: 2020 × End date: 2023 × Type: Article ×

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    PublicationOpen Access
    Triterpenoids and steroids isolated from Anatolian Capparis ovata and their activity on the expression of inflammatory cytokines
    (2020-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Semen, Sevcan; Acar, Ozden Ozgun; KOLAK, Ufuk; Sen, Alaattin; TOPÇU, GÜLAÇTI; GAZİOĞLU, IŞIL; TOPÇU, GÜLAÇTI
    Context: Capparis L. (Capparaceae) is grown worldwide. Caper has been used in traditional medicine to treat various diseases including rheumatism, kidney, liver, stomach, as well as headache and toothache. Objective: To isolate and elucidate of the secondary metabolites of the C. ovata extracts which are responsible for their anti-inflammatory activities. Materials and methods: Buds, fruits, flowers, leaves and stems of C. ovata Desf. was dried, cut to pieces, then ground separately. From their dichloromethane/hexane (1:1) extracts, eight compounds were isolated and their structures were elucidated by NMR, mass spectroscopic techniques. The effects of compounds on the expression of inflammatory cytokines in SH-SY5Y cell lines were examined by qRT-PCR ranging from 4 to 96 mM. Cell viability was expressed as a percentage of the control, untreated cells. Results: This is a first report on isolation of triterpenoids and steroids from C. ovata with anti-inflammatory activity. One new triterpenoid ester olean-12-en-3b,28-diol, 3b-pentacosanoate (1) and two new natural steroids 5a,6a-epoxycholestan-3b-ol (5) and 5b,6b-epoxycholestan-3b-ol (6) were elucidated besides known compounds; oleanolic acid (2), ursolic acid (3), b-sitosterol (4), stigmast-5,22-dien-3b-myristate (7) and bismethyl-octylphthalate (8). mRNA expression levels as EC10 of all the tested seven genes were decreased, particularly CXCL9 (19.36-fold), CXCL10 (8.14-fold), and TNF (18.69) by the treatment of 26 mM of compound 1 on SH-SY5Y cells. Discussion and conclusions: Triterpenoids and steroids isolated from C. ovata were found to be moderate-strong anti-inflammatory compounds. Particularly, compounds 1 and 3 were found to be promising therapeutic agents in the treatment of inflammatory and autoimmune diseases.
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    Investigation of cytotoxic and apoptotic effects of 63 compounds obtained from Salvia species: Promising anticancer agents
    (2022-05-01T00:00:00Z) Kandemir, Sevgi Irtegun; FİDAN, HİLAL SARUHAN; Yener, Ismail; METE, NURİYE; ERTAŞ, ABDULSELAM; TOPÇU, GÜLAÇTI; KOLAK, Ufuk; TOPÇU, GÜLAÇTI
    Since ancient time, Salvia L. species have been commonly used to treat colds, bronchitis, tuberculosis, heart diseases, and menstrual and digestive disorders in traditional medicine all around the world. They have been also used as tea and spice. Studies indicated that diterpenes and triterpenes isolated from Salvia species possess various pharmacological and biological effects such as anti-inflammatory, antiviral, cytotoxic, antioxidant, and hepatotoxic activities. Flavones were also shown to have antimicrobial, antioxidant, and cytotoxic potentials. Salvia extracts also exhibit anti-Alzheimer, antiseptic, cardiovascular, antihypertensive, and antituberculous effects. To investigate the effects of 63 secondary metabolites from Salvia species on cell viability and apoptosis, Salvia secondary metabolites including 25 phenolics, 4 fatty acids, 19 abietane diterpenoids, 12 triterpenoids, and three steroids were examined on healthy cell line (PDF), breast cancer (MCF-7), and colon cancer (HT-29) cell lines using MTT method. In addition, the effects of rosmarinic acid, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, carnosic acid, carnosol, cryptotanshinone, beta-sitosterol, and ursolic acid on pro-apoptotic Bax and antiapoptotic Bcl-2 protein expression levels were investigated by Western Blot method. Practical applications Phenolic compounds (apigenin, chrysin, and luteolin) and diterpenes (especially dihydrotanshinone I, carnosic acid, and carnosol), and almost all of the triterpenes exhibited high toxic effects on healthy cell line. Cytotoxic effects of cryptotanshinone, 12-hydroxy abieta-1,3,5(10),8,11,13-hexaene, 12-demethylmulticauline, 6,7-dehydroroyleanone, acetyl royleanone, ferruginol, ursolic acid, and 3-acetyl lupeol were relatively higher than their toxic effects. Acetyl royleanone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone were found to have anticancer potential based on their modulating effects on the expression levels of Bax and Bcl-2 proteins which play important roles in the regulation of apoptosis. The results of the present study showed that acetyl royleanone, cryptotanshinone, 6,7-dehydroroyleanone, carnosic acid, and cryptotanshinone have potential to be used in the pharmaceutical industry.
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    Di-, and Triterpenoids Isolation and LC-MS Analysis of Salvia marashica Extracts with Bioactivity Studies
    (2021-11-01T00:00:00Z) Toraman, Gulbahar O. Alim; Aydin, Sibel Kiran; Ertas, Abdulselam; Boga, Mehmet; EROL, EBRU; Saygi, Tuba Kusman; Halfon, Belkis; TOPÇU, GÜLAÇTI; EROL, EBRU; ALİM TORAMAN, GÜLBAHAR ÖZGE; TOPÇU, GÜLAÇTI
    In this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salvia marashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of these extracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidant activity was determined by four complementary methods including inhibition of lipid peroxidation (by beta-carotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity and CUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity tests were carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. The triterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone and methanol extracts isolated by chromatographic methods. Their structures were determined based on spectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone or methanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate, lup-12, 20(29)-diene, lup-20(29)-ene, alpha-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroid beta-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene (2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in the present study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MS analysis.
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    Chemical composition and biological activities of Cypriot propolis
    (2021-09-01T00:00:00Z) NALBANTSOY, AYŞE; SARIKAHYA, NAZLI; Ozverel, Cenk Serhan; Barlas, Ayse Bercin; Kirci, Damla; AKGÜN, İSMAİL HAKKI; Yalcin, Tansel; Duven, Gamze; KIŞLA, DUYGU; DEMİRCİ, BETÜL; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN
    Propolis compositions are highly variable, depending on the geographic region and the season of collection. In this study, propolis samples from seven different regions of Cyprus were studied for the first time by means of chemical content and biological activities. Secondary metabolite composition was determined by LC-HRMS. While the major flavonoids found were isosakuranetin, naringenin, rhamnocitrin, diosmetin, chrysin and acacetin, interestingly verbascoside, a phenylethanoid glycoside, and chlorogenic acid were identified as the major compounds in the ethanol-water extracts. alpha-Pinene was detected as the major compound of propolis extracts according to the volatile compositions via GC-MS. Karaoglanoglu and Tirmen extracts, presenting different chemical profiles, exerted enormous cytotoxic activity by MTT assay (IC50: 2.36-11.56 mu g/mL; 1.44-9.33 mu g/mL, respectively). The highest iNOS inhibition potential was detected in the Karpaz extract (IC50:2.6 mu g/mL) in LPS induced RAW 264.7 cells whereas the Guzelyurt sample demonstrated remarkable antioxidant (88.82 +/- 0.10%) and antimicrobial activities (with a MIC value of 31.2 mu g/mL against S. aureus, S. epidermidis, E. faecium, and E. faecalis).
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    PublicationOpen Access
    Special issue dedicated to the memory of Professor Ayhan Ulubelen (1931-2020)
    (2021-01-01T00:00:00Z) TOPÇU, GÜLAÇTI; TOPÇU, GÜLAÇTI
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    PublicationOpen Access
    Bioguided Isolation of Secondary Metabolites from Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa
    (2021-11-01T00:00:00Z) Ertas, Abdulselam; Cakirca, Hatice; Yener, Ismail; Akdeniz, Mehmet; Fırat, Mehmet; TOPÇU, GÜLAÇTI; KOLAK, Ufuk; TOPÇU, GÜLAÇTI
    In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemic species, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and tested for determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, and cupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolated from the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4-hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2-dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone (11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid (15), oleanolic acid (16), taraxasterol (17), lupenone (18), beta-sitosterol (19), and stigmasterol (20). Rosmarinic acid, which was obtained from the aerial parts, was found to be the best antioxidant compound among the isolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays (IC50: 1.20 +/- 0.04 mu g/mL, IC50: 1.74 +/- 0.06 mu g/mL, A(0.5): 1.22 +/- 0.02 mu g/mL, respectively). Chlorogenic and caffeic acids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidant activity in the mentioned assays.
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    Ent-kaurene Diterpenoids from Sideritis lycia with Antiviral and Cytotoxic Activities
    (2020-07-01T00:00:00Z) Kilic, Turgut; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; AYDOĞMUŞ, Zeynep; KARAGÖZ, Ali; Yildiz, Yasar K.; Aslan, Irfan; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN
    The genus Sideritis (Lamiaceae) is represented by 45 species (54 taxa) in Anatolia with high endemism ratio (74%), and Turkey is one of the gene centers of the genus along with Spain. Acetone extract of the aerial parts of Sideritis lycia afforded eight known ent-kaurene diterpenoids, structures of which have been identified as linearol, isolinearol, isosidol, sidol, siderol, sideridiol, 7-epi-candicandiol and foliol through 1H NMR, 13 C NMR and mass spectroscopic analyses. Cytotoxic and antiviral activities of the acetone extract, linearol, sidol and isosidol were investigated together with insecticidal activity of species. The antiviral index of linearol, isosidol and acetone extract of S. lycia were determined as 2.31, 2.01 and 2.58, respectively, except sidol. 7-Epi-candicandiol was found to be the most active diterpene against a series of cancer cell lines with ED50 values; KB (13.3 mu g/mL), COL-2 (11.8 mu g/mL), LU1 (17.9 mu g/mL), LNCaP (14.9 mu g/mL) and A2780 (9.0 mu g/mL). Activity results of this study indicated that ent-kaurane diterpenes have potential to be considered as antiviral and cytotoxic lead compounds.
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    Comparing healing effect against ulcerative colitis and toxicological effects of Rosmarinus officinalis: A comprehensive in vivo study of an edible plant in rats
    (2022-07-01T00:00:00Z) YILMAZ, ANIL; UÇKAYA, FATİH; BAYINDIR, NİHAN; GÜLER, ERAY METİN; TOPRAK, ALİ; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDES; TOPÇU, GÜLAÇTI; UÇKAYA, FATİH; BAYINDIR, NİHAN; GÜLER, ERAY METİN; TOPRAK, ALİ; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDES; TOPÇU, GÜLAÇTI
    Ulcerative colitis (UC) is a chronic and inflammatory disorder of the gastrointestinal (GI) tract. UC usually worsens the daily life of the patient and may sometimes become mortal. There is no known remedy discovered against UC, yet. Rosmarinus officinalis consists of many flavonoids, phenolics, and terpenoids possessing various biological activities such as anti-inflammatory. For this reason, in the present study, anti-ulcerative colitis effect of ROME (Rosmarinus officinalis methanol extract) was investigated comprehensively by histopathological studies, a number of in vivo anti-inflammatory activities and several in vivo antioxidant activities, in addition to in vitro antioxidant activities and biochemical analyses. In addition, the toxic effects of ROME were examined. The results showed that ROME provided a significant healing effect against ulcerative colitis in rats. Both in vitro and in vivo assay results correlated with histopathological examinations. ROME exhibited minimal toxic alterations. When the results of rosemary are compared with the results of sulfasalazine, it can be suggested that instead of synthetic drugs with side effects, natural sources can be used for the treatment of various diseases. Although some activities of rosemary have been investigated in vitro in the previous studies, this is the first study revealing anti-ulcerative colitis effect of rosemary through histopathological studies, in vivo and in vitro assays as well as biochemical analyses overall. Practical applications The results revealed and proved that ROME provided a significant healing effect against ulcerative colitis in rats. When the results of rosemary are compared with the results of sulfasalazine, a commercially available drug on the market, it can be suggested that instead of synthetic drugs with side effects, natural sources can be used for the treatment of various inflammatory diseases such as UC disease. In addition, ROME possesses limited toxic alterations, but not much more than the commercial drug. As a future perspective, lethal and therapeutic doses can be examined and determined. Thus, human studies can be started through this comprehensive in vivo study on rosemary which is commonly used as an edible plant and spice all over the world.
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    A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum
    (2020-02-01T00:00:00Z) Dincel, Demet; Topçu, Gülaçtı; DİNCEL, DEMET; TOPÇU, GÜLAÇTI
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    Composition of the essential oil of Satureja metastasiantha: a new species for the flora of Turkey
    (2020-01-01T00:00:00Z) Carikci, Sema; GÖREN, AHMET CEYHAN; Dirmenci, Tuncay; Yalcinkaya, Burhanettin; Erkucuk, Aysen; TOPÇU, GÜLAÇTI; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI
    The aerial parts of Satureja metastasiantha were hydrodistilled for 3 h using a Clevenger-type apparatus. The essential oils were analyzed by gas chromatography/flame ionization detector and gas chromatography/mass spectrometry, simultaneously, the main compounds of which were characterized as p-cymene (22.3%), thymol (21.0%), carvacrol (18.45%), and gamma-terpinene (12.1%). Antioxidant capacity, acetylcholinesterase and butyryl-cholinesterase inhibition effects, and antimicrobial and antifungal properties of the species were evaluated. The anticholinesterase activity of the essential oil of S. metastasiantha was observed with 30% inhibition at 200 mu g/mL. The essential oil of the species showed activity against Staphylococcus aureus with 128 mu g/mL minimum inhibitory concentration value.