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GÖNCÜ, BEYZA SERVET

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BEYZA SERVET
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GÖNCÜ
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End date: 2022 × Start date: 2020 × Item Type: Publication × Type: Article × Rights: info:eu-repo/semantics/openAccess ×

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Now showing 1 - 4 of 4
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    PublicationOpen Access
    Investigating differential miRNA expression profiling using serum and urine specimens for detecting potential biomarker for early prostate cancer diagnosis
    (2021-02-08T00:00:00Z) Hasanoğlu, Sevde; Göncü, Beyza Servet; Yücesan, Emrah; Atasoy, Sezen; Kayali, Yunus; Özten Kandaş, Nur; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; ATASOY, SEZEN
    Background/aim: MicroRNAs (miRNAs) are known up-to-date candidate biomarkers for several diseases. In addition, obtaining miRNA from different body fluids such as serum, plasma, saliva, and urine is relatively easy to handle. Herein we aimed to detect miRNAs as biomarkers for early stage prostate cancer (PC). For this purpose, we used urine and serum samples to detect any significant differences in miRNA profiles between patients and healthy controls. Materials and methods: Total ribonucleic acid (RNA) in urine and serum samples were isolated from eight untreated PC patients, thirty healthy individuals were screened for miRNA profile, and candidate miRNAs were validated. Whole urinary and serum miRNA profile was analyzed using Affymetrix GeneChip miRNA 4.0 Arrays. Candidate miRNAs were investigated by stem-loop reverse transcription- polymerase chain reaction. Results: When we analyzed the urinary samples of PC patients, 49 miRNAs were detected to be upregulated and 14 miRNAs were found to be downregulated when compared with healthy controls. According to the serum samples, 19 miRNAs were found to be upregulated, and 21 miRNAs were found to be downregulated when compared with healthy individuals as well. Interestingly, we detected only four overlapping miRNAs (MIR320A, MIR4535, MIR4706, MIR6750) that commonly increase or decrease in both serum and urine samples. Among them, MIR320A was found to be downregulated, and MIR4535, MIR4706, and MIR6750 were found to be upregulated for urine samples. However, only MIR6750 was upregulated and the other three miRNAs were downregulated for serum samples. Conclusion: Notably, the expression profile of MIR320A was significantly altered in urine specimens of prostate cancer patients. We considered that MIR320A has been evaluated as a valuable biomarker that can be used in the early diagnosis of PC.
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    Hücre Mikroenkapsülasyonunda Manuel ve Kapsülasyon Sisteminin Hücre İzolasyon Tipi ve Aljinat Yüzdesine Bağlı Verimliliğinin Karşılaştırılması
    (2021-08-01T00:00:00Z) Düzenli, Ömer Faruk; Göncü, Beyza Servet; Selepcioğlu, Harika; Yücesan, Emrah; Ersoy, Yeliz Emine; Akçakaya, Adem; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; ERSOY, YELIZ EMINE; AKÇAKAYA, ADEM
    Amaç: Birçok endüstriyel sektörde kullanılan polimer malzemeler sağlık bilimlerinde de farklı işlemlerde kullanılmaktadır. Bu işlemlerden biri olan enkapsülasyon sistemi hücre nakli gibi terapötik uygulamalarda tercih edilmektedir. Enkapsülasyon çalışmalarında uygulanacak yaklaşıma göre kapsül yapısında kullanılacak polimer malzeme ve oluşan kapsül boyutu değişmektedir. Aljinat, kahverengi alglerden elde edilen, içeriğindeki farklı polimerik blok oranlarına bağlı olarak değişiklik gösteren doğal polimerlerden biridir. Bu çalışmada, kapsülasyon aşaması için kullanılacak olan değişik aljinat yüzdeleri uygulanarak paratiroid hücrelerinde ideal mikroenkapsülasyon prosedürlerinin belirlenmesi amaçlanmaktadır.Gereç ve Yöntem: Çalışmada sekonder hiperparatiroidi hastasından alınan bir adet paratiroid hiperplazi dokusundan mekanik ve enzimatik izolasyon yöntemleriyle hücre eldesi gerçekleştirilmiştir. İki farklı aljinat yüzdesi kullanarak hem manuel olarak hem de kapsülasyon cihazında otomatize olarak iki farklı akış hızı değerlendirilmiştir. Mikroenkapsüle edilen hücreler 64-79 gün boyunca in vitro olarak parathormon miktarları ölçülerek takip edilmiştir.Bulgular: Değerlendirilen aljinat yüzdelerinden %2’lik konsantrasyona sahip mikroenkapsüllerin oluşturulmasında kapsülasyon cihazında kullanılan 2 mL/dk akış hızıyla morfolojik stabilite gözlenmiştir. Ayrıca parathormon salınımı açısından hücre izolasyon tipi ve aljinat yüzdeleri arasında benzer sonuçlar elde edilmiştir.Sonuç: Uzun süreli mikroenkapsülasyon verimliliğinin arttırılması için yapısal ve fonksiyonel açıdan birçok parametrenin belirlenmesi gerekmektedir. Bu çalışma ile enzimatik izolasyon metoduyla elde edilen paratiroid hücrelerinin kapsülasyon sistemi kullanılarak artan akış hızında daha stabil bir yapı oluşturdukları belirlenmiştir.
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    PublicationOpen Access
    Xenotransplantation of Microencapsulated Parathyroid Cells as a Potential Treatment for Autoimmune-Related Hypoparathyroidism.
    (2021-08-09T00:00:00Z) Yucesan, Emrah; Basoglu, Harun; Goncu, Beyza; Gul, Burcu; Aysan, Erhan; Ersoy, Yeliz Emine; GÖNCÜ, BEYZA SERVET; YÜCESAN, EMRAH; GÜL, BURCU
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    PublicationOpen Access
    Differential anti-proliferative and apoptotic effects of lichen species on human prostate carcinoma cells
    (2020-09-01T00:00:00Z) Goncu, Beyza; SEVGİ, ECE; KIZILARSLAN HANÇER, ÇAĞLA; Gokay, Guzin; ÖZTEN KANDAŞ, NUR; GÖNCÜ, BEYZA SERVET; SEVGİ, ECE; KIZILARSLAN HANÇER, ÇAĞLA; ÖZTEN KANDAŞ, NUR
    Lichens are stable symbiotic associations between fungus and algae and/or cyanobacteria that have different biological activities. Around 60% of anti-cancer drugs are derived from natural resources including plants, fungi, sea creatures, and lichens. This project aims to identify the apoptotic effects and proliferative properties of extracts ofBryoria capillaris (Ach.) Brodo&D.Hawksw,Cladonia fimbriata (L.) Fr.,Evernia divaricata (L.) Ach.,Hypogymnia tubulosa (Schaer.) Hav.,Lobaria pulmonaria (L.) Hoffm., andUsnea florida (L.) Weber ex Wigg. lichen species on prostate cancer cells. Lichen extracts were performed by ethanol, methanol, and acetone separately by using the Soxhlet apparatus and the effects of the extracts on cell viability, proliferation, and apoptosis were measured with the utilization of MTT, LDH assay, Annexin V assay, and Western Blot. Findings of our study revealed a positive correlation between the elevation of cell sensitivity and the increase in the treatment doses of the extract in that higher doses applied reverberate to higher cell sensitivity. A similar correlation was also identified between cell sensitivity elevation and the duration of the treatment. Evidence in our study have shown the existence of an anti-proliferative effect in the extracts ofBryoria capillaris,Evernia divaricata (L.) Ach.,Hypogymnia tubulosa (Schaer.) Hav.,Lobaria pulmonaria (L.) Hoffm., andUsnea florida (L.) Weber ex Wigg., while a similar effect was not observed in the extracts ofCladonia fimbriata.Evernia divaricatainduced anti-proliferative and apoptotic effects in PC-3 cells,whichinduced apoptotic cell death by both extrinsic and intrinsic pathways.Hypogymnia tubulosahas been shown to have anti-proliferative and apoptotic effects in all extractions methods and our findings identified that both the percentage of the apoptotic cells and apoptotic protein expressions recorded an increase at lower treatment concentrations. AlthoughLobaria pulmonariais known to have significant cytotoxic effects, we did not observe a decrease in cell proliferation. Indeed, proliferation marker proliferating cell nuclear antigen (PCNA) protein expression levels have shown an increase in all extracts, whileUsnea florida exhibitedapoptosis induction and slight proliferation reduction in extract treatments with lower concentrations. We tested 18 extracts of six lichen species during our study. Of these,Evernia divaricataandHypogymnia tubulosademonstrated significant apoptotic activity on prostate cancer cells including at low concentrations, which implies that it is worth pursuing the biologically active lead compounds of these extracts on prostate cancerin vitro. Further corroboratory studies are needed to validate the relative potential of these extracts as anti-metastatic and anti-tumorigenic agents.