Person:
GÖREN, AHMET CEYHAN

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Kurumdan Ayrılmıştır

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Organizational Unit

Eczacılık Fakültesi

First Name

AHMET CEYHAN

Last Name

GÖREN

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Now showing 1 - 10 of 15

Open Access
Antidiabetic, anticholinergic and antioxidant activities of aerial parts of shaggy bindweed (Convulvulus betonicifolia Miller subsp.) - profiling of phenolic compounds by LC-HRMS
(2021-05-01T00:00:00Z) Bingol, Zeynebe; Kızıltaş, Hatice; GÖREN, AHMET CEYHAN; Kose, Leyla Polat; Topal, Meryem; DURMAZ, LOKMAN; Gulcin, İlhami; Alwasel, Saleh H.; GÖREN, AHMET CEYHAN
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH. scavenging activity, ABTS(center dot)(-) scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, alpha-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase and 0.675 mu g/mL against alpha-amylase enzymes.
Open Access
The phenolic contents, antioxidant and anticholinesterase activity of section Amaracus (Gled.) Vogel and Anatolicon Ietsw. of Origanum L. species
(2020-04-01T00:00:00Z) Ozer, Zuleyha; GÖREN, AHMET CEYHAN; Kilic, Turgut; Oncu, Merve; Carikci, Sema; Dirmenci, Tuncay; GÖREN, AHMET CEYHAN
Origanum boissieri Ietsw., O. saccatum P.H.Davis, O. solymicum P.H.Davis and O. ayliniae Dirmenci & T.Yazici belonging to sect. Amaracus (Gled.) Vogel, O. sipyleum L. and O. hypericifolium O.Schwarz & P.H.Davis belonging to sect. Anatolicon Ietsw. were analyzed for their chemical composition of essential oil and phenolic components. The essential oil compositions were analysed by using GC-MS and GC-FID. The phenolic contents of the chloroform, acetone, and methanol extracts were analyzed using LC-MS/MS. Antioxidant activities of the extracts were investigated by using three methods; DPPH free radical scavenging activity, beta-carotene linoleic acid assays and CUPRAC assays. The essential oil compositions of the section Amaracus were found to be as carvacrol type (O. ayliniae, O. boissieri) and p-cymene type (O. saccatum, O. solymicum). In the section of Anatolicon, while O. sipyleum was found as gamma-terpinene type, O. hypericifolium was carvacrol type. In the extracts, the most abundant components were determined as flavonoids, coumaric acids and derivatives. Especially rosmarinic acid and penduletin were detected in high amounts. Among the studied species, extracts of O. ayliniae showed quite good activity for all methods. The extracts from all species showed remarkable antioxidant activity. Inhibition capability of the extracts against acetyl and butyrylcholinesterase enzymes (AChE and BChE) were determined. The extracts were found as inactive against AChE. The moderate inhibition capacity observed against BChE. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
Open Access
Saponins from Twenty-Two Cephalaria Species
(2021-11-01T00:00:00Z) Sarikahya, Nazli Boke; GÖREN, AHMET CEYHAN; Okkali, Gaye Sumer; Kirmizigul, Suheyla; GÖREN, AHMET CEYHAN
Saponins in the Cephalaria species have a broad spectrum of biological properties and application potentials. This research aimed to develop a novel method to tentatively identify and quantify triterpenes and saponins in 22 Cephalaria species by LC-HR/MS method. In this study, we provided a broad screening of important biologically active triterpenes and saponins with high separation ability, high sensitivity and high selectivity. This is the first report to prove that Cephalaria species can be a good source of tormentic acid which has many biological activities in literature. The main triterpene compound, tormentic acid was presented at the level between 74.02 mg/kg and 75702.0 mg/kg in n-butanol extract of Cephalaria species. The source of some biologically active saponins such as davisianoside B, aristatoside C, elmalienoside A, balansoide B and scoposide B were also determined with this study, for the first time. Therefore, due to the different activities and usages of the identified compounds, these findings may find application in a number of industries such as nutraceutical, pharmaceutical, medical and cosmetic industries.
Open Access
Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS
(2021-11-01T00:00:00Z) Kızıltaş, Hatice; GÖREN, AHMET CEYHAN; Bingol, Zeynebe; Alwasel, Saleh H.; GÜLÇİN, İlhami; GÖREN, AHMET CEYHAN
To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS(center dot+) scavenging activity, DPPH. scavenging activity, Fe(3+)reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, alpha-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase, and 0.675 mu g/mL against alpha-amylase.
Open Access
LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer
(2021-10-01T00:00:00Z) Kızıltaş, Hatice; Bingol, Zeynebe; Goren, Ahmet C.; Pınar, Süleyman Mesut; Alwasel, Saleh H.; GÜLÇİN, İlhami; GÖREN, AHMET CEYHAN
Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than 250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425 taxa. The roots of Asfragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial, antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing. For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Asfragalus brachycalyx FISCHER (EEAB), some bioanalytic methods including DPPH center dot and ABTS(center dot+) scavenging effects, ferric ions (Fe3+) and cupric ions (Cu2+) reducing abilities, and metal (Fe-) chelating activity were realized. alpha-Tocopherol, ascorbic acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 mu g/mL toward acetylcholinesterase (AChE), 0.620 mu g/mL on alpha-glycosidase and 0.306 mu g/mL against alpha-amylase enzymes.
Open Access
Anticholinesterase furocoumarins from Heracleum platytaenium, a species endemic species to Ida Mountains
(2013-01-01) Dincel, DEMET; Hatipoglu, Seda Damla; GOREN, AHMET CEYHAN; Topcu, Gulacti; DİNCEL, DEMET; GÖREN, AHMET CEYHAN; TOPÇU, GÜLAÇTI
The petroleum ether extract of Heracleum platytaenium afforded 8 furocoumarins (psoralen, bergapten, xanthotoxin, pimpinellin, isopimpinellin, sphondin, byakangelicin, and heraclenol) and the methanol extract of H. platytaenium gave only 1 glycosylated dihydrofurocoumarin, apterin. In addition, stigmasterol was also obtained from petroleum ether extract. Structure identification of the isolated compounds has been achieved by using spectroscopic methods, namely 1D and 2D NMR experiments and mass spectral analyses. The antioxidant activity of the extracts and pure compounds was investigated by 2 methods, including DPPH free radical scavenging activity and lipid peroxidation inhibitory activity by beta-carotene-linoleic acid assays. The anticholinesterase activity of petroleum ether and methanol extracts of the plant and the isolated furocoumarins was investigated against acetylcholinesterase and butyrylcholinesterase enzymes by the Ellman method in vitro.
Open Access
Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds
(2020-10-01T00:00:00Z) ÖZGEN, UFUK; ŞENER, SILA ÖZLEM; Smejkal, Karel; Vaclavik, Jiri; ŞENOL DENİZ, FATMA SEZER; ERDOĞAN ORHAN, İLKAY; Svajdlenka, Emil; GÖREN, AHMET CEYHAN; Zemlicka, Milan; GÖREN, AHMET CEYHAN
Objectives: The scope of the present study was to specify the therapeutic potential for neurodegenerative diseases through evaluating cholinesterase and tyrosinase (TYR) inhibitory and antioxidant activity of Lysimachia verticillaris (LV), and to isolate the major compounds considering the most active fraction.
Open Access
Anticholinergic, antidiabetic and antioxidant activities of cinnamon (cinnamomum verum) bark extracts: polyphenol contents analysis by LC-MS/MS
(2019-01-01) GÜLÇİN, İlhami; Kaya, Ruya; GÖREN, AHMET CEYHAN; Akincioglu, Hulya; Topal, Meryem; Bingol, Zeynebe; Cakmak, Kader Cetin; Sarikaya, Sevim B. Ozturk; DURMAZ, LOKMAN; Alwasel, Saleh; GÖREN, AHMET CEYHAN
Ethanolic (EEC) and aqueous (WEC) extracts of cinnamon (Cinnamomum verum) were evaluated for their antioxidant profiles by eight distinguished bioanalytical antioxidant methods. Their inhibitory effects were tested against some enzymes including acetylcholinesterase, butyrylcholinesterase, alpha-glycosidase and alpha-amylase, which linked to different diseases. Additionally, the antioxidant properties were determined and polyphenolic compositions of the both extracts were evaluated by LC-MS/MS analysis. According to the LC-MS/MS experiments, thirteen compounds were found in WEC and EEC. Also, p-hydroxybenzoic acid (321.1 mg/kg extract), p-coumaric acid (291.4 mg/kg extract), and pyrogallol (142.4 mg/kg extract) were found to be the most abundant ingredients in the WEC. On the other hand, pyrogallol (264.3 mg/kg extract), ferulic acid (224.7 mg/kg extract) and p-coumaric acid (170.2 mg/kg extract) were found as the most plentiful chemicals in the EEC. For the estimation of the antioxidant capacities of the both extracts (WEC and EEC), DPPH center dot and ABTS(center dot+) scavenging activities, as well as Fe3+-Fe2+, and Cu2+-Cu+ reducing assays were studied. The IC50 values of the WEC and EEC indicated that they were potent effective DPPH center dot (21. 25 and 15.71 mu g/mL) and ABTS(center dot+) (6.52 and 5.79 mu g/mL) scavengers, as well as AChE (221.33 and 110.26 mu g/mL), BChE (461.69 and 94.93 mu g/mL), alpha-glycosidase (206.86 and 220.00 mu g/mL) and alpha-amylase (189.86 and 200.86 mu g/mL) inhibitors. As a conclusion, both EEC and WEC had rich phenolic contents and demonstrated effective anticholinergic, antidiabetic and antioxidant effects.
Open Access
Anti-Alzheimer, antidiabetic and antioxidant potential of Satureja cuneifolia and analysis of its phenolic contents by LC-MS/MS
(2020-03-01T00:00:00Z) Taslimi, Parham; KÖKSAL, EKREM; GÖREN, AHMET CEYHAN; Bursal, Ercan; Aras, Abdulmelik; Kilic, Omer; Alwasel, Saleh; GÜLÇİN, İlhami; GÖREN, AHMET CEYHAN
Many chronic diseases such as diabetes and Alzheimer-s disease are related to the type and quality of foods, which are consumed. Particularly, various plant origin products are stated as beneficial against such kind of chronic diseases with secondary metabolites such as their phenolic structures. Satureja cuneifolia is a plant, which is consumed as an herbal tea in some regions of Turkey and that-s why investigate of its biological activity is important. In our study, the anti-diabetic and anti-Alzheimer potentials of the methanol and water extracts of S. cuneifolia plant were measured via some enzymes inhibition experiments as in vitro. The antioxidant ability of the same extracts was measured via radical scavenging and reducing power methods. Also, the total phenolics and flavonoids of the plant were identified. Finally, the extracts were analyzed by the LC-MS/MS analysis and the phenolic content of S. cuneifolia was clarified. (C) 2019 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
Open Access
Anticholinergic and antioxidant activities of avocado (Folium perseae) leaves - phytochemical content by LC-MS/MS analysis
(2020-01-01T00:00:00Z) Kose, Leyla Polat; Bingol, Zeynebe; Kaya, Ruya; GÖREN, AHMET CEYHAN; Akincioglu, Hulya; DURMAZ, LOKMAN; KÖKSAL, EKREM; Alwasel, Saleh H.; GÜLÇİN, İlhami; GÖREN, AHMET CEYHAN
In the first stage of the manuscript, we aimed to examine antioxidant capacity and anticholinergic properties of avocado (Folium perseae) leaves. Avocado leaf was extracted by water (WEFP) and ethyl alcohol (EEFP) and antioxidant activity was determined using by several antioxidant assays including DPPHGreek ano teleia and ABTS(center dot+)radical scavenging assays, Cu2+-Cu(+)reducing, Fe3+-Fe(2+)reducing, and FRAP reducing activities. Avocado leaf extracts demonstrated antioxidant activity and anticholinergic activities, while alpha-tocopherol, BHT, trolox, and BHA were used as positive antioxidant controls. In the second part of this study, the inhibition effects of WEFP and EEFP were valuated against acetylcholinesterase and butyrylcholinesterase enzymes, which catalyze the breakdown of choline esters (i.e. neurotransmitters). This study obviously showed that avocado leaf extracts had effective antioxidant, antiradical, and anticholinergic influences.