Gümüş eklenmiş çeşitli simanların antibakteriyel etkinliklerinin ve materyal bağlanma dayanımının invitro olarak incelenmesi / Evaluation of the antibacterial effectiveness and the bonding strength of various silveradded cement materials in vitro
AuthorYILDIRIM, Zeynep Beyza
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The aim of this in-vitro work is to examine the physical and antibacterial properties of the orthodontic tapes by adding lyophilized Ag nanoparticles (silver) to the conventional glass ionomer, resin-modified glass ionomer adhesive cement and polyacid modified composite resin materials used for the cementation of orthodontic tapes. In our study, the orthodontic bands were composed of conventional glass ionomer (Ketac Cem), resin modified glass ionomer (Multi-Cure) and polyacid modified composite resin (Transbond Plus) band cements and a total of 138 teeth were formed by the addition of 0.1% lyophilized Ag nanoparticles. The six month aging procedure was applied to the specimens to be subjected to the mechanical test. The desynthesizing of the bands with the 'Instron device (TSTM 02500, Elista, Turkey) was carried out by applying tensile force. The maximum force exerted on the break was measured with the aid of a fixed force gauge attached to the upper part of the instrument and recorded in N (Newton) with the computer connected to the instrument. The values obtained in Newtonian terms were then converted to megapask using the equation Mpa = N / mm², and the statistical analyzes were evaluated as MPa (megapascal). Multi Cure cement without Ag nanoparticle showed the highest tensile strength values; The difference between all groups except the Multi-Cure cement containing Ag nanoparticle was found to be statistically significant (p<0,05). Ketac Cem cement containing Ag nanoparticle; Although higher tensile strength than the Transbond Plus groups and Ag nanoparticle-free the Ketac Cem group without Ag nanoparticles, the only statistically significant difference was seen with the Ag nanoparticles containing Transbond Plus cement (p <0.05). The Ketac Cem feldspar containing Ag nanoparticles is followed by the Transbond Plus groups which do not contain Ag nanoparticles and Ketac Cem and Ag nanoparticles with respectively. Statistically significant difference for both cements was obtained with Multi Cure groups (p <0.05). The lowest tensile strength values were recorded for the Transbond Plus cement containing Ag nanoparticles. At the same time, there was showed a statistically significant difference between the Multi Cure groups and the Ketac Cem groups containing Ag nanoparticles (p<0,05). When the intrinsic tensile strength values were examined, it was seen that the addition of Ag nanoparticle to cement materials did not significantly affect the in-group tensile strength values in the statistically significant level. The cementum residue remaining on the surface of the tooth after the desaturation was visually inspected and scored according to the residual adhesive index (ARI) used by Artun and Bergland. According to the results of the ARI score evaluation, the band cementation break was observed mostly in the Ketac Cem group with and without Ag nanoparticles. It was also seen that the band cement breakage was high in the glass ionomer groups with and without Ag nanoparticles. The highest tensile strengths are found in the adhesive over 50% of the tooth, and the lowest tensile strength is measured in samples that do not have any adhesive on the tooth. Disc diffusion method, liquid medium culture and biofilm formation methods were used for antibacterial evaluation. Discs were produced with the help of Teflon discs with a diameter of 2 mm and a diameter of 9 mm. Antimicrobial properties of all materials were examined by disk diffusion method and Ag nanoparticle was added and examined without addition and no inhibition zone was observed. No difference was observed in CIS as it appeared to reduce the St mutans colonization by RMCIS supplemented with Ag nanoparticles and PMKR added to the liquid medium. Most of the biofilm formation was observed with conventional glass ionomer, Ag nanoparticle-added conventional glass ionomer and component simulator, compared to the absorbance readings measured at 550 nm wavelength in the ELISA reader.