Research Project:
Identification of gut microbiome of individuals who live in Turkey, pilot study

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11.2018\16

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Description

The human gut harbours a complex ecosystem composed of 1014 microbial cells. More than 1,500 different bacterial species have already been associated with the human gut microbiota. The close relationship between the host and microbial community causes intestinal and extra-intestinal disorders. Over the last two decades, development of next-generation sequencing (NGS) technology has significantly increased our knowledge of gut microbiota. Tools permitting exhaustive analysis of individual gut microbiota including a phylogenetic identification and quantification are still under development. Most of these techniques are based on the 16S ribosomal RNA (rRNA) gene sequence variations between different species. 16S rRNA sequencing is a common, cost-effective, amplicon sequencing method that can be analysed to determine the bacterial taxa and gives accurate measures of taxonomic diversity. The aim of this project is to identify taxonomic classes of human microbes and to reveal their association with diseases. Stool samples will be collected from experimental and control groups, DNA will be isolated from the samples and next generation sequencing of the obtained DNA samples based on the 16s rRNA gene regions of the bacterial populations that constitute the microbiota. Statistical analysis will be performed to compare the results between test and control groups. As a result, we will categorize microbiota of patients and healthy individuals in Turkish populations. This classification will allow identification of new interactions between diseases and gut microbiome. These novel links will be used to develop therapeutic targets and diseases and treatment approaches in the long term.

Keywords

Sağlık Bilimleri
Temel Bilimler
Health Sciences
Natural Sciences
Klinik Tıp (MED)
Temel Bilimler (SCI)
Doğa Bilimleri Genel
ÇOK DİSİPLİNLİ BİLİMLER
Clinical Medicine (MED)
Natural Sciences (SCI)
NATURAL SCIENCES, GENERAL
MULTIDISCIPLINARY SCIENCES
Multidisciplinary