Person: DOYMAZ, MEHMET ZIYA
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Publication Open Access Screening of mecC Gene in Methicillin Resistant Staphylococcus aureus Isolates(2022-04-01T00:00:00Z) Ceylan, Ayse Nur; SÜMBÜL, BİLGE; DOYMAZ, Mehmet Ziya; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAObjective: The diagnosis and treatment of mecC positive methicillin resistant Staphylococcus aureus (MRSA) isolates pose a significant problem in clinical microbiology and infectious disease practices. The studies on the frequency of mecC positive isolates in Turkey is rather scarce. In the present study, we aimed to investigate the presence of mecA, mecC, spa and pvu genes in MRSA strains isolated from various clinical specimens submitted to Clinical Microbiology Laboratories of Bezmialem Vakıf Hospital. Methods: We performed nucleic acid extraction and multiplex polymerase chain reaction (PCR) to 126 MRSA strains to detect mecC, mecA, spa and pvl genes. Results: According to the multiplex PCR results of 126 MRSA strains studied, 126 (100%) had mecA, 107 (85%) had spa, and 3 (2%) had pvl genes. We performed another polymerase chain reaction protocol and spa genes were identified in 19 of specimens, which were found negative priorly. Conclusion: Considering the factors that a university medical center where the study was conducted provided a tertiary healthcare service to a large metropolitan area in Istanbul and none of the isolates carried mecC gene might indicate that mecC gene carrying MRSA isolates did not pose a significant public health threat in Turkey.Publication Metadata only Assesment of Predetory Traits of Bdellovibrio bacteriovorus and Agromyces ramosus Species on Clinical Pathogens(2019-08-19) Doymaz M. Z.; Karaaslan E.; Kurtoğlu A. V.; DOYMAZ, MEHMET ZIYA; KURTOĞLU, AHMET VOLKANPublication Open Access Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)(2021-12-01T00:00:00Z) Karaaslan, Elif; Kalkan-Yazıcı, Merve; Hasanoğlu, Sevde; Karakeçili, Faruk; Özdarendeli, Aykut; Kalkan, Ahmet; Kılıç, Ali Osman; Doymaz, Mehmet Ziya; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYAIn 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations.Publication Metadata only The Utility of CRISPR/Cas9 Technology to Attenuate Apoptotic Pressure in Monoclonal Antibody (mAb) Producing Cells(2022-10-20) Doymaz M. Z.; Aydın B. N.; Çetin N. S.; Kaygusuz A.; DOYMAZ, MEHMET ZIYA; ÇETİN, NESİBE SELMAPublication Metadata only SERUM PARAOXONASE ACTIVITY AND PHENOTYPE DISTRIBUTION IN TURKISH COVID-19 PATIENTS(2021-12-03T00:00:00Z) Gülen, Bedia; Selek, Şahabettin; Çelik, Halil İsa; Taşlıdere, Bahadır; Sönmez, Ertan; Metin, Hüseyin; Sarıkaya, Ufuk; Okay, Gülay; Doymaz, Mehmet Ziya; Kazancıoğlu, Rümeyza; SELEK, ŞAHABETTİN; TAŞLIDERE, BAHADIR; SÖNMEZ, ERTAN; OKAY, GÜLAY; DOYMAZ, MEHMET ZIYA; KAZANCIOĞLU, RÜMEYZAPublication Open Access Hand, Foot, and Mouth Disease Caused by Coxsackievirus A6: A Preliminary Report from Istanbul.(2019-01-01) Ceylan, AN; Turkmen, AV; Turel, O; Gultepe, BİLGE; Inan, E; Doymaz, MEHMET ZİYA; TÜREL, ÖZDEN; SÜMBÜL, BİLGE; VEHAPOĞLU TÜRKMEN, AYSEL; DOYMAZ, MEHMET ZIYAHand, foot, and mouth disease (HFMD) is caused by various serotypes of Enterovirus genus. Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) were known to be the only responsible agents for these epidemics; however, this opinion was challenged after the detection that coxsackievirus A6 (CV-A6) was the responsible species for the outbreak in Finland in 2008. HFMD is frequently seen in Turkey, and no detailed study on its clinical and microbiological epidemiology has previously been reported. The present study addresses this question. Twenty-seven patient samples collected between 2015 and 2017 were included in the study. Typing was conducted by RT-PCR and the sequencing applied directly to patient's samples and as well as to the viral cultures with pan-enterovirus and serotype-specific primers. The presence of Enterovirus in 12 of 27 HFMD samples was shown with RT-PCR. The causative agent for three of these 12 samples was CV-A16, one of the most frequent two serotypes around the world, and the remaining nine samples was CV-A6. The findings of the study are relevant since it pertains to the molecular epidemiology of HFMD in Turkey, a gateway country where different serotypes might be circulating and transmitted. The findings also support the notion that CV-A6 cases are rising in number, which has caused more severe clinical features and widespread rashes in recent outbreaks. Hand, foot, and mouth disease (HFMD) is caused by various serotypes of Enterovirus genus. Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) were known to be the only responsible agents for these epidemics; however, this opinion was challenged after the detection that coxsackievirus A6 (CV-A6) was the responsible species for the outbreak in Finland in 2008. HFMD is frequently seen in Turkey, and no detailed study on its clinical and microbiological epidemiology has previously been reported. The present study addresses this question. Twenty-seven patient samples collected between 2015 and 2017 were included in the study. Typing was conducted by RT-PCR and the sequencing applied directly to patient’s samples and as well as to the viral cultures with pan-enterovirus and serotype-specific primers. The presence of Enterovirus in 12 of 27 HFMD samples was shown with RT-PCR. The causative agent for three of these 12 samples was CV-A16, one of the most frequent two serotypes around the world, and the remaining nine samples was CV-A6. The findings of the study are relevant since it pertains to the molecular epidemiology of HFMD in Turkey, a gateway country where different serotypes might be circulating and transmitted. The findings also support the notion that CV-A6 cases are rising in number, which has caused more severe clinical features and widespread rashes in recent outbreaks.Publication Metadata only [Intestinal strongyloidiasis in a psoriasis patient with diabetes].(2014-06-01T00:00:00Z) Iraz, Meryem; Karaman, Ulkü; Topukçu, Buğçe; Doymaz, Mehmet Ziya; DOYMAZ, MEHMET ZIYAPublication Metadata only COVID-19 ENFEKSİYONUN MİKROBİYOLOJİSİ(Bezmialem Vakıf Üniversitesi Yayınları, 2021-01-01) DOYMAZ M. Z.; SÜMBÜL B.; AKBAŞ E.; KARAASLAN E.; ÇETİN N. S.; YAZICI M.; DOYMAZ, MEHMET ZIYA; SÜMBÜL, BİLGE; ÇETİN, NESİBE SELMA; YAZICI, MERVEPublication Open Access A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey(2020-01-01T00:00:00Z) Doymaz, MZ; Sumbul, BİLGE; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYADespite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered to be endemic for TB. In this study, we analyzed the distribution of Mycobacterium species, compare the diagnostic methods, and susceptibilities to anti-tuberculosis drugs of TB isolates. The aim was to document the current status and to provide a frame of reference for future studies. In this study, 278 Mycobacterium species isolated from 7,480 patients between September 2015 and June 2019 were included. Löwenstein-Jensen medium (LJ) and MGIT 960 were used for the isolation of strains. Susceptibility to 1st-line anti-tuberculosis drugs was determined. Positivity rates in clinical samples were as follows: 1.4% for direct microscopic acid-fast bacilli (AFB) detection, 3.4% for growth on the LJ, and 3.7% for growth on MGIT-960. Two hundred thirty-three isolates were identified as Mycobacterium tuberculosis complex (MTBC) and 45 were non-tuberculous mycobacteria (NTMs). Eleven of the NTMs (24.4%) were Mycobacterium fortuitum group isolates, and eight NTMs (17.7%) were Mycobacterium abscessus complex isolates. A number of patients diagnosed with tuberculosis peaked twice between the ages of 20–31 and 60–71. A hundred and eighty-two MTBC isolates (78.1%) were susceptible to all 1st-line anti-tuberculosis drugs, while 51 isolates (21.9%) were resistant to at least one drug tested. The multidrug-resistant tuberculosis rate was 13.7% among resistant strains and 3% in all strains. The liquid cultures were better for detection of both MTBC and NTMs isolates. The data demonstrate that MTBC continues to be challenge for this country and indicates the need for continued surveillance and full-spectrum services of mycobacteriology laboratory and infectious diseases.Publication Metadata only Identification of an atypical strain of toxoplasma gondii as the cause of a waterborne outbreak of toxoplasmosis in Santa Isabel do Ivai, Brazil.(2010-10-15T00:00:00Z) Vaudaux, JD; Muccioli, C; James, ER; Silveira, C; Magargal, SL; Jung, C; Dubey, JP; Jones, JL; Doymaz, MEHMET ZİYA; Bruckner, DA; Belfort, R; Holland, GN; Grigg, ME; DOYMAZ, MEHMET ZIYA