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Now showing 1 - 10 of 11
  • Publication
    Effect of Breast Milk Calcium and Fluidity on Breast Cancer Cells: An In Vitro Cell Culture Study
    (2016-11-01T00:00:00Z) Bayram, Recep; Yavuz, Muhsine Zeynep; Benek, Bedri Selim; BOZKURT, Aysenur Aydogar; UCBEK, Ali; Ozunal, Zeynep Gunes; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    Aim: The aims of this study were to investigate the effects of calcium at the same concentration as that found in human milk on the viability, proliferation, and adhesion of MCF-7 human breast ductal carcinoma cells by exposing them to calcium at the same frequency as in breastfeeding.
  • Publication
    Effect of metformin on the human T98G glioblastoma multiforme cell line
    (2014-05-01T00:00:00Z) Ucbek, Ali; Ozunal, Zeynep Gunes; Uzun, Ozge; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    Metformin is a guanidine derivative found in Galega officinalis that is commonly used to treat diabetes mellitus. The mechanism of action of metformin involves regulation of the adenosine monophosphate-activated protein kinase/mammalian target of rapamycin signaling pathway, which is implicated in the control of protein synthesis and cell proliferation. This led to the hypothesis that metformin reduces the risk of cancer and slows tumor growth. Thus, in the present study, the effectiveness of metformin as an antiglioma agent was evaluated using the human T98G glioblastoma multiforme cell line. The viability of the T98G cells was assessed using a 3-(4,5-dimethylthiazol-2-yL)-2,5-diphenyltetrazolium bromide assay. Apoptosis was monitored by measuring caspase-3 levels, as well as by terminal deoxynucleotidyl transferase dUTP nick end labeling and staining with acridine orange and ethidium bromide. The results demonstrate that metformin reduced cell viability and caused apoptotic morphological changes in the T98G cells. Furthermore, the caspase-3 levels in the metformin-treated T98G cells were higher than those in the control cells. Metformin induced apoptosis in the T98G cell line in a concentration-dependent manner. Metformin may provide an important contribution to the treatment of glioblastoma multiforme.
  • Publication
    Possible Cytoprotective Potential of Ruthenium Red in Evaluation of the Rapid Apoptotic Model Induced by H2O2
    (2011-04-01T00:00:00Z) Bayram, Recep; Yavuz, Muhsine Zeynep; Duzenli, Selma; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    In the present paper, hydrogen peroxide (H2O2) induced an apoptotic process in 1 h and over the doses of 10 mM, while cisplatin did not, in cortical neuronal homogenates of rats. It was proved in DNA fragmentation, MTT and WST-1 assays. Especially, according to WST-1 assay results, apoptotic effect was decided to be very obvious in all tests in the doses of 20 mM of the rapid model of apoptosis of H2O2. Ruthenium red, as a mitochondrial Ca2+ modulator, was tested alone and co-application with H2O2. Without H2O2, at low doses of it, ruthenium red seems to have a slight viability inducing effect in respect to controls in MTT and WST-1, despite that this effect was not observed clearly in DNA fragmentation test. Another point is that the highest dose of ruthenium red (10 M), induces apoptosis, even stronger than the 20 mM H2O2, controversy to the general believing.
  • Publication
    ACE-2, TMPRSS2 and Beyond; Promising Targets and Tools for COVID-19 Prophylaxis and Treatment
    (2020-12-01T00:00:00Z) Gepdiremen, Ali Akçahan; Kumaş, Meltem; GEPDİREMEN, ALİ AKÇAHAN
    Several repurposing drugs and ongoing vaccine researches tocombat Coronavirus Disease-19 (COVID-19) are testing clinically,worldwide. COVID-19 caused by severe acute respiratory failuresyndrome-CoV-2, uses angiotensin-converting enzyme 2 (ACE-2) as a functional receptor for entry into the cells, followed by itspriming by transmembrane protease serine 2 (TMPRSS2). Most ofthe ACE-2 expressing cells are alveolar type II pneumocytes. ViralS-glycoprotein, TMPRSS2 and ACE-2 inhibition, as extracellularmedia components, are potential targets of future therapy. ACE-2 and/or TMPRSS2 blockade is thought to be beneficial in theprevention or treating of this infection which will be the mostconvenient for pharmacoeconomics and effectiveness, regardingsimilar future pandemics. Despite substrate-based design andsynthesis of ACE-2 inhibitor compounds were presented almosttwo decades ago, data on renin angiotensin system activation orits blockers, especially ACE-2, are limited by now. Priority mustbe given to design a convenient vaccine soon, but due to the highmutation ability of such viruses mean that new vaccines may needto be developed for each outbreak. So, de novo drugs such as ACE-2or TMPRSS2 blockers need to be developed which can specificallyblock spike binding sites of the target cells and prevent virusintrusion, especially at the extracellular media, for future pandemics.
  • Publication
    Synthesis of novel triazoles bearing 1,2,4-oxadiazole and phenylsulfonyl groups by 1,3-dipolar cycloaddition of some organic azides and their biological activities
    (2014-01-01T00:00:00Z) Durust, Yasar; Karakus, Hamza; Yavuz, Muhsine Zeynep; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    1,3-Dipolar cycloaddition of 5-azidomethyl-3-p-substituted phenyl-1,2,4-oxadiazoles to phenyl vinyl sulfone and bismaleimide gives rise straightforwardly to 1-((3-(p-substituted) phenyl-1,2,4-oxadiazol-5-yl)methyl)-4-(phenylsul-fonyl)- 4,5-dihydro-1 H -1,2,3-triazoles and bisdihydropyrrolo[3,4-d][1,2,3] triazole-4,6(3 a H, 5 H)-diones. The structures of the new cycloadducts were elucidated by means of IR, NMR (1 H, 1 3 C, 2D), mass spectra, and physical characteristics (mp and R f values). In addition, anticancer activities of the cycloadducts against MCF-7 cells were also investigated.
  • Publication
    Antiproliferative Activity of Some Medicinal Plants on Human Breast and Hepatocellular Carcinoma Cell Lines and their Phenolic Contents
    (2015-10-01T00:00:00Z) Karakas, Fatma Pehlivan; Yildirim, Arzu Birinci; Bayram, Recep; Yavuz, Muhsine Z.; Gepdiremen, ALİ AKÇAHAN; Turker, Arzu Ucar; GEPDİREMEN, ALİ AKÇAHAN
    Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis, Convolvulus galaticus, Trifolium pannonicum and Lysimachia vulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines.
  • Publication
    Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line
    (2014-01-01T00:00:00Z) Kaya, Ertugrul; Bayram, Recep; Yaykasli, Kursat Oguz; YILMAZ, Ismail; Bayram, Sait; Yaykasli, Emine; Yavuz, Muhsine Zeynep; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    Background/aim: Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line.
  • Publication
    The evaluation and comparison of the alpha and beta amanitin toxicity on MCF-7 cell line
    (2013-07-01T00:00:00Z) Kaya, Ertugrul; Bayram, Recep; Yaykasli, Kursat Oguz; YILMAZ, Ismail; Bayram, Sait; Yaykasli, Emine; Yavuz, Muhsine Zeynep; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
  • Publication
    Apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and kit
    (2012-08-01T00:00:00Z) GÜLÇİN, İlhami; Beydemir, Sukru; Topal, Fevzi; Gagua, Natia; Bakuridze, Aliko; Bayram, Recep; Gepdiremen, ALİ AKÇAHAN; GEPDİREMEN, ALİ AKÇAHAN
    In the present study, apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and Kit were studied. For testing the possible apoptotic effects of majdine and isomajdine from V. herbacea, DNA fragmentation assay was conducted on the rat brain cortical tissue homogenates, in vitro. Also their possible effects on mitochondrial activity were tested by using the same tissue samples of rats. In addition, the antioxidant activity of isomajdine and majdine was determined using various in vitro antioxidant assays, including 2,2--azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging and N,N-dimethyl-p-phenylenediamine (DMPD center dot+) radical scavenging, ferric ions (Fe3+) and cupric ions (Cu2+) reducing abilities and ferrous ions (Fe2+) chelating activity. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were used as reference antioxidants.
  • Publication
    Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline
    (2010-02-01T00:00:00Z) GÜLÇİN, İlhami; elias, Riad; Gepdiremen, ALİ AKÇAHAN; Chea, Aun; Topal, Fevzi; GEPDİREMEN, ALİ AKÇAHAN
    In the present study, we determined the antioxidant activity of cepharanthine and fangchinoline from Stephania rotunda by performing different in vitro antioxidant assays, including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2--azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging, superoxide anion (O-2(center dot-)) radical scavenging, hydrogen peroxide scavenging, total antioxidant activity, reducing power, and ferrous ion (Fe2+) chelating activities. Cepharanthine and fangchinoline showed 94.6 and 93.3% inhibition on lipid peroxidation of linoleic acid emulsion at 30 mu g/mL concentration, respectively. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, and trolox indicated inhibitions of 83.3, 92.2, 72.4, and 81.3% on peroxidation of linoleic acid emulsion at the same concentration (30 mu g/mL), respectively. According to the results, cepharanthine and fangchinoline have effective antioxidant and radical scavenging activity.