2015-02-01T00:00:00Z, Eldeniz, Ayce Unverdi, Guneser, MEHMET BURAK, Akbulut, Makbule Bilge, GÜNEŞER, MEHMET BURAK

The aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.

2019-01-01, AKBULUT, MB, Guneser, MEHMET BURAK, ELDENIZ, AU, GÜNEŞER, MEHMET BURAK

2019-09-11T00:00:00Z, ÜNVERDİ ELDENİZ, AYÇE, AKBULUT, MAKBULE BİLGE, GÜNEŞER, MEHMET BURAK, GÜNEŞER, MEHMET BURAK

2017-12-01T00:00:00Z, Akbulut, Makbule Bilge, Terlemez, Arslan, Akman, Melek, Buyukerkmen, Begum, Guneser, Mehmet Burak, Eldeniz, Ayce Unverdi, GÜNEŞER, MEHMET BURAK

Background/purpose: Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this in vitro study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration.

2017-01-01, Baka, Zeliha Muge, Eldeniz, Ayce Unverdi, Guneser, MEHMET BURAK, Basciftci, Faruk Ayhan, GÜNEŞER, MEHMET BURAK

Aim: The aim of this study was to evaluate the antibacterial effects of five orthodontic bonding materials commonly used in orthodontics. Materials and Methods: The antibacterial effects of five orthodontic bonding materials were evaluated against Streptococcus mutans and Streptococcus salivarius by direct contact test (DCT). Using the DCT technique, quintet specimens of Transbond XT, Blugloo, Grengloo, Light Bond, and Opal Bond were applied to the bottom and the walls of the five wells of a 96-microtiter plate with a height of 2 mm. Samples were prepared in two sets: 1-day samples and 7-day samples. Ten microliters of bacterial suspension were added to each well for direct contact with each material for 1h at 37 degrees C. Bacterial growth was then measured in a microplate spectrophotometer hourly at 600 nm for 24h. Five uncoated wells with identical inoculum sizes served as positive controls. The data obtained at the end of 24h was statistically analyzed with one-way ANOVA, and post hoc comparisons were done using Tamhane-s T2 test. Results: Blugloo generally showed better antibacterial activity than the other materials. Transbond XT did not show any antibacterial activity. There was a statistically significant difference between Transbond XT and Light Bond in the 1-day sample against S. mutans (p0.05). Conclusion: Blugloo had the most potent antibacterial activity against S. salivarious.

2017-02-01, GÜNEŞER, MEHMET BURAK, GÜNEŞER, MEHMET BURAK

2019-01-01T00:00:00Z, ALİM, BETÜL AYCAN, YUCA, TUĞÇE, GÜNEŞER, MEHMET BURAK, GÜNEŞER, MEHMET BURAK

2013-03-01T00:00:00Z, Guneser, MEHMET BURAK, Akbulut, Makbule Bilge, Eldeniz, Ayce Unverdi, GÜNEŞER, MEHMET BURAK

Introduction: The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fosses, France) in comparison with contemporary root perforation repair materials. Methods: Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to-be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. Results: Biodentine showed significantly higher push-out bond strength than MTA (P amalgam >= IRM >= Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. Conclusions: Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin. (J Endod 2013;39:380-384)

2017-06-01, Dincer, ASİYE NUR, Guneser, MEHMET BURAK, ARSLAN, Dilara, GÜNEŞER, MEHMET BURAK, DİNÇER, ASİYE NUR

Objective: The aim of this in vitro study was to compare the various techniques for removing precipitate formed after irrigation with sodium hypochlorite (NaOCl) and chlorhexidine (CHX). Background data: It is still unclear whether different irrigant activation systems can effectively remove precipitate that is firmly attached to the root canal walls. Methods: Eighty-two extracted single-rooted human teeth were instrumented by using the ProTaper rotary system. The roots were irrigated with 5% NaOCl and then 2% CHX to form orange-brown precipitate on the root canal walls. The teeth were sectioned longitudinally, and the amount of precipitate on the canal walls was measured under a stereomicroscope at 15xmagnification before the root halves were reassembled. Then, conventional syringe irrigation (CSI), CanalBrush (CB), EndoActivator (EA) system, photon-induced photoacoustic streaming (PIPS), and manual instrumentation (MI) techniques were used to remove the precipitate, during which 5mL of distilled water was used for 1min. The amount of precipitate remaining on the canal walls was measured, and the percentage of precipitate removed was calculated. Data were analyzed by using the Kruskal-Wallis and Mann-Whitney U tests (p=0.05). Results: All experimental groups contained residual precipitate. The precipitate was more effectively removed in the MI group than in the other groups (p0.05). Conclusions: None of the techniques used was able to completely remove the orange-brown precipitate from the root canal surfaces.

2016-05-29T00:00:00Z, GÜNEŞER, MEHMET BURAK, DİNÇER, ASİYE NUR, ARSLAN, DİLARA, GÜNEŞER, MEHMET BURAK