Person: GÜNEŞER, MEHMET BURAK
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Publication Metadata only Comparative antifungal efficacy of light-activated disinfection and octenidine hydrochloride with contemporary endodontic irrigants(2015-02-01T00:00:00Z) Eldeniz, Ayce Unverdi; Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; GÜNEŞER, MEHMET BURAKThe aim of this study was to evaluate the antifungal effects of light-activated disinfection (LAD) in comparison with contemporary root canal irrigation solutions: sodium hypochlorite and 2 % chlorhexidine gluconate and a new wound antiseptic, octenidine hydrochloride. Seventy extracted teeth having single root canals were contaminated with Candida albicans for 14 days. The samples were divided into five experimental (n = 10) and two control (positive and negative) groups (n = 10): (1) LAD with toluidine blue O, (2) octenidine hydrochloride (OCT), (3) 2.5 % sodium hypochlorite (2.5 % NaOCl), (4) 5.25 % sodium hypochlorite (5.25 % NaOCl) and (5) 2 % chlorhexidine. Five millilitres of each test solution was applied for 3 min, and irradiation time used for LAD was 30 s. After treatment, the dentin chips were collected from inner canal walls into vials containing phosphate buffered saline, vortexed, serially diluted, seeded on Tryptic Soy Agar plates and incubated (37 A degrees C, 48 h). The number of colony-forming units was then counted. Differences between LAD group and positive control group were statistically significant (P < 0.05). All Candida cells were totally eliminated in root canals irrigated with OCT, 2.5 % NaOCl, 5.25 % NaOCl and 2 % chlorhexidine groups (CFU = 0). Within the limitations of this ex vivo study, LAD had minimal antimicrobial effect on C. albicans when used 30 s, and further modifications in LAD protocol are required to improve its antifungal capability. A new wound antiseptic, octenidine hydrochloride, demonstrated better potential than LAD in elimination of Candida albicans cells and may be a promising alternative to NaOCl and chlorhexidine solutions in future.Publication Metadata only Effect of Various Endodontic Irrigants on the Push-out Bond Strength of Biodentine and Conventional Root Perforation Repair Materials(2013-03-01T00:00:00Z) Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAKIntroduction: The aim of this study was to evaluate the effect of various endodontic irrigants on the push-out bond strength of Biodentine (Septodont, Saint Maur des Fosses, France) in comparison with contemporary root perforation repair materials. Methods: Midroot dentin of canine teeth was horizontally sectioned into 1-mm-thick slices. The canal space of each dentin slice was enlarged with a diamond bur to 1.4 mm in diameter. The samples were divided into 5 groups (n = 40), and the following materials were placed, respectively: Biodentine, ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), amalgam, Dyract AP (Dentsply DeTrey, Konstanz, Germany), and intermediate restorative material (IRM, Dentsply DeTrey). The samples were wrapped in wet gauze for 10 minutes and divided into 3 subgroups (n = 10) to-be immersed into 3.5% sodium hypochlorite, 2% chlorhexidine gluconate (CHX), or saline for 30 minutes. No irrigation was performed in the controls (n = 10), and a wet cotton pellet was placed over each test material. After incubation for 48 hours, the dislodgement resistance of the samples was measured using a universal testing machine. The samples were examined under a stereomicroscope to determine the nature of the bond failures. Results: Biodentine showed significantly higher push-out bond strength than MTA (P amalgam >= IRM >= Biodentine > MTA. The push-out bond strength of Dyract AP, amalgam, IRM, and Biodentine was not significantly different when immersed in NaOCl, CHX, and saline solutions, whereas MTA lost strength when exposed to CHX. Conclusions: Biodentine showed considerable performance as a perforation repair material even after being exposed to various endodontic irrigants, whereas MTA had the lowest push-out bond strength to root dentin. (J Endod 2013;39:380-384)Publication Metadata only Are dentin posts biomechanically intensive?: A laboratory and FEA study(2014-12-17) Belli, Sema; Celik, Kezban; Akbulut, Makbule Bilge; Guneser, MEHMET BURAK; Eraslan, Oguz; Eskitaccioglu, Gurcan; GÜNEŞER, MEHMET BURAKThe goal of this laboratory and finite elemental stress analysis (FEA) study was to compare fracture strength of dentin post (DP) with stainless steel and glass fiber posts. Single-rooted teeth were decoronated and restored with ParaPost (PP), i-TFC (TFC), and DPs (n=10). Resin cores were created and loaded until failure (1.0mm/min). The data were analyzed (ANOVA, Tukey, Chi-Square tests). Three-dimensional FEA models of the posts were created and the stress distributions were calculated using Solid Works/Cosmos works structural analysis program. A significant difference was found among the groups (p<0.001). TFC group showed the highest and the DP group showed the lowest fracture resistance (p<0.001). PP created more stress at the root dentin and inside the post. Restoration of roots with glass fiber and DPs may reduce stresses within the core material and the remaining root. However, a fracture-resistant restoration cannot be achieved by using a DP.Publication Metadata only Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs(2016-03-03) Akman, Melek; Akbulut, Makbule Bilge; Guneser, MEHMET BURAK; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAKTo evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole-ciprofloxacin-cefaclor, Group2: a combination of metronidazole-ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P=0.00). The medications have an effect on the push-out bond strength of both materials (P=0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.Publication Open Access Antibacterial effect of chlorhexidine-cetrimide combination, Salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants(2016-10-01) Guneser, MEHMET BURAK; Akbulut, Makbule Bilge; Eldeniz, Ayce Unverdi; GÜNEŞER, MEHMET BURAKThe aim of the present study was to compare the antimicrobial effect of sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), a CHX/cetrimide solution (CHX+CTR), octenidine hydrochloride (OCT) and Salvia officinalis plant extract against Enterococcus faecalis. Seventy decoronated single-rooted human teeth were infected and divided into 6 test (n=10) and 2 control groups (n=5) (negative, sterile samples and positive, infected samples). Following irrigants were then applied to test groups: 2.5% NaOCl, 5.25% NaOCl, CHX, CHX+CTR, S. officinalis extract and OCT. The dentin chips were obtained from inner root canal walls and analyzed by counting the number of colony forming units (CFU). The 2.5% NaOCl, 5.25% NaOCl, CHX and OCT groups presented no bacterial growth (CFU=0). S. officinalis and CHX+CTR groups reduced the number of E. faecalis cells but could not eliminate all. OCT may have potential as an endodontic irrigant in treatment of infected root canals.