Person: SÜSGÜN, SEDA
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Publication Metadata only Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.(2019-07-01) Kucukkaya, B; Basoglu, H; Erdag, D; Akbas, FAHRİ; Yalcintepe, L; AKBAŞ, FAHRİ; SÜSGÜN, SEDAIntracellular calcium concentration ([Ca2+](i)) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high success rate in cancer therapies. We investigated the regulatory role of [Ca2+]in cisplatin resistance in epithelial ovarian cancer cell line, in MDAH-2774, and its chemoresistant subclone MDAH-2774/DDP. The measurement of [Ca2+](i) using fluorescence microscope, and flow cytometry revealed that the amount of intracellular calcium decreased in cisplatin resistant cells compared to the amounts in parental cells. mRNA expression profiles of calcium homeostasis-associated major genes (IP(3)R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) decreased in cisplatin resistant cell line in comparison to the expression profiles in parental cells. Owing to the changes in the expression of genes involved in calcium regulation, these results show, drug resistance may be prevented by introducing a new perspective on the use of inhibitors and activators of these genes, and thus of cytostatic treatment strategies, due to changes in the expression of genes involved in calcium regulation.Publication Open Access The utility of serum microRNA-93 and microRNA-191 levels for determination of injury severity in adults with multiple blunt trauma(2020-12-01T00:00:00Z) Sogut, Ozgur; Metiner, Merve; Kaplan, Onur; Calık, Mustafa; Cakmak, Sumeyye; Umit, Tugba Betul; Ergenc, Huseyin; Akbas, Fahri; Süsgün, Seda; AKBAŞ, FAHRİ; SÜSGÜN, SEDABACKGROUND: Various scoring systems have been developed to determine the trauma severity and prognosis of patients following multiple blunt trauma (MBT). However, these scoring systems do not provide exactly the desired severity assessment. In recent years, serum concentration of many specific miRNAs, especially for head trauma, has been shown to play an important role in determining the diagnosis, severity and prognosis of injury. To date, however, no studies have investigated serum microRNAs in patients with MBT. Thus, this study measured the expression of miRNA-93 and -191 in the serum of adults with MBT and examined the correlations of Injury Severity Score (ISS) and Revised Trauma Score (RTS) values with serum miRNA-93 and -191 levels in these patients with the aim of predicting trauma severity based on the miRNA levels.METHODS: This prospective case–control study enrolled 50 consecutive adults with MBT and age- and sex-matched 60 healthy controls. The patients were divided into ISS >16 (group 1, major or severe trauma) and ISS ≤16 (group 2, minor or mild-moderate trauma) groups. Serum miRNA-93 and -191 levels were assessed using quantitative real-time reverse transcription-PCR. We evaluated whether the miRNAs were differentially expressed in major and minor MBT patients and determined their utility for assessing the severity of injury.RESULTS: The mean serum miRNA-93 and -191 levels were significantly elevated in the patients compared to the controls and were higher in patients with ISS >16 compared to those with ISS ≤16, although the difference was not significant. In the patients with multitrauma, ISS was significantly, negative and weak correlated with serum miRNA-191 level (rho = –0.320, p = 0.023) but not with the serum miRNA-93 level. No optimal cutoff for the serum miRNA-93 level was found with respect to trauma severity (AUC 0.617, [0.455–0.779]). However, an optimal cutoff value for serum miRNA-191 was identified, with values <1.94 indicating severe trauma (AUC 0.668 [0.511–0.826]; 65.6% sensitivity, 77.8% specificity).CONCLUSION: miRNA-191 and -93 levels were significantly upregulated in multitrauma patients compared to controls. The level of miRNA-191 in conjunction with ISS, but not that of miRNA-93, may be a useful biomarker for determining injury severity in patients with multitrauma.Publication Open Access Fibromiyalji Sendromu ile Bağırsak Mikrobiyotası Arasındaki İlişkinin Araştırılması(2021-03-01T00:00:00Z) Albayrak, Büşra; Süsgün, Seda; Küçükakkaş, Okan; Akbaş, Fahri; Yabacı, Ayşegül; Özçelik, Semra; SÜSGÜN, SEDA; KÜÇÜKAKKAŞ, OKAN; AKBAŞ, FAHRİ; YABACI TAK, AYŞEGÜL; ÖZÇELİK, SEMRAFibromiyalji sendromu (FMS), yetişkin popülasyonda bildirilen prevalansı %3-10 olan, kronik yaygın ağrının sık görülen formlarından biridir. Tipik ağrının klinik görünümü ve ilişkili somatik ve psikolojik semptomların varlığı tanıya temel oluşturur. FMS sinir sistemi disfonksiyonu ile ilişkilidir ve nörotransmitterler fibromiyalji için onaylanmış bir dizi ilacın hedefi olarak rol oynamaktadır. Bununla birlikte, FMS’de altta yatan mekanizmalar kesin olarak henüz bilinmemekle birlikte birçok hipotez ortaya konmuştur. Fibromiyalji ve irritabl bağırsak sendromu (IBS) arasındaki ilişki göz önüne alındığında, değişen bağırsak mikrobiyomu fibromiyalji ile ilişkili olabilir. Bu çalışmada, FMS tanılı hastalarda sağlıklı kontrollere göre değişen bağırsak mikrobiyom düzeylerinin araştırılması amaçlanmıştır. Mikrobiyom çalışması için FMS tanılı 54 hastadan ve 36 sağlıklı kişiden oluşan kohorttan fekal örnekler toplanmıştır. Kontrol grubunda herhangi bir mental ve/veya fiziksel hastalığı olanlar çalışma dışında bırakılmıştır. FMS’li hasta grubu, -American College of Rheumatology (ACR)- 2010 tanı kriterlerine bağlı kalınarak belirlenmiştir. Fekal örnekler, kullanılana kadar -80°C’de muhafaza edilmiş ve buz üzerinde çözülmüş; her ekstraksiyon için 0.3 g feçes tartılmıştır. DNA izolasyonu, ticari kit ile üreticinin protokolüne uygun olarak gerçekleştirilmiştir. Örnekler, gerçek zamanlı polimeraz zincir yöntemi ile Bacteroidetes, Firmicutes, Enterobacter, Lactobacillus, Streptococcus ve Bifidobacterium’a özgül primerler ile 16S rRNA gen amplifikasyonu yapılarak karşılaştırılmıştır. Sonuçlara göre, hasta grubunda Bacteroidetes ve Bifidobacterium istatistiksel olarak anlamlı bir şekilde artarken (p< 0.05), Firmicutes’in azaldığı saptanmıştır (p< 0.001). Enterobacter, Streptococcus ve Lactobacillus için istatistiksel olarak anlamlı sonuç bulunamamıştır (p> 0.05). Bakteriler arasındaki ilişki değerlendirildiğinde, Bacteroidetes ile Firmicutes yüzdesi arasında istatistiksel olarak yüksek anlamlı ve negatif korelasyon bulunurken (r= -0.778, p< 0.001), Enterobacter ve Bifidobacterium yüzdesi arasında orta derecede istatistiksel anlamlılık ve pozitif korelasyon gözlenmiştir (r= 0.460, p= 0.005). Sonuçlar, bağırsak mikrobiyotasının fibromiyaljide rol oynayabileceğini göstermektedir. Bağırsaktaki Firmicutes ve Bacteroidetes filumlarının dengesinin bağırsak homeostazı için önemli etkilere sahip olduğu bilinmektedir. Özetle, daha büyük kohortlarda yapılacak büyük ölçekli araştırmaların, bağırsak mikrobiyomu ve FMS arasındaki ilişkiyi anlamada ve olası tedavi seçeneklerini değerlendirmede etkili olacağı açıktır.Publication Metadata only Possible effects of caffeic acid phenethyl ester and thymoquinone against toluene brain injury(2019-08-31T00:00:00Z) MEYDAN, SEDAT; EŞREFOĞLU, MUKADDES; SELEK, ŞAHBETTİN; AKBAŞ TOSUNOĞLU, ESRA; öztürk, özlem; kurbetli, nuriye; BAYINDIR, NİHAN; BULUT, HURİ; MERAL, İSMAİL; SÜSGÜN, SEDA; AKBAŞ, FAHRİ; MEYDAN, SEDAT; EŞREFOĞLU, MUKADDES; SELEK, ŞAHABETTİN; MERAL, İSMAİL; SÜSGÜN, SEDA; AKBAŞ, FAHRİPublication Metadata only Effects of Methylprednisolone in the Treatment of Spinal Cord Injuries by Evaluation of microRNA-21: An Experimental Study.(2022-04-19T00:00:00Z) Abdallah, Anas; Tekin, Abdurrahim; Oztanir, Mustafa Namik; Süsgün, Seda; Yabacı, AYŞEGÜL; Çınar, İrfan; Can, Engin; Tokar, Sadık; Akbaş, Fahri; Seyithanoğlu, Mehmet Hakan; SÜSGÜN, SEDA; YABACI TAK, AYŞEGÜL; AKBAŞ, FAHRİ; SEYİTHANOĞLU, MEHMET HAKANPublication Metadata only Fibromiyalji Sendromu ile Bağırsak Mikrobiyotası Arasındaki İlişkinin Araştırılması(2020-12-31T00:00:00Z) Albayrak, Büşra; Süsgün, Seda; Küçükakkaş, Okan; Akbaş, Fahri; Yabacı, Ayşegül; Özçelik, Semra; SÜSGÜN, SEDA; KÜÇÜKAKKAŞ, OKAN; AKBAŞ, FAHRİ; YABACI TAK, AYŞEGÜLFibromyalgia syndrome (FMS) is one of the frequent forms of chronic widespread pain, with a reported prevalence of 2% to 4% in the adult population. Nervous system dysfunction is implicated in FMS and neurotransmitters are the targets of a number of drugs approved for fibromyalgia. However exact underlying mechanisms of FMS are unknown yet and multiple hypotheses being suggested. Considering the relation between fibromyalgia and irritable bowel syndrome, altered gut microbiome could be associated with fibromyalgia. Herein, altered gut microbiome levels were investigated in patients with FMS compared to healthy controls. We collected faeces to study the microbiome, from a cohort of 54 patients with FMS and 36 healthy individuals. Extraction of DNA was carried out with commercial kit according to the manufacturer’s recommendations. Samples were compared using 16S rRNA gene amplification with specific primers of Bacteroidetes, Firmicutes, Enterobacteria, Lactobacillus, Streptococcus and Bifidobacterium by the Real-Time PCR instrument. According to our results, Bacteroidetes and Bifidobacteria were increased statistically significant (p<0.05), Firmicutes was decreased (p<0.001) in the patient group. No statistically significant results were found for Enterobacterium, Streptococcus and Lactobacillus (p>0.05). When the relationship between bacteria was evaluated, statistically significant and negative correlation was found between Bacteroidetes and Firmicutes percentage (r =-0.778, p <0.001). A moderately positive correlation was observed between the percentage of Enterobacter and Bifidobacter (r = 0.460, p = 0.005). The results suggest that the gut microbiota may play a role in fibromyalgia.t the gut microbiota may play a role in fibromyalgia. In sum, it is clear that large-scale further research in larger cohorts will be efficient in understanding the relationship of gut microbiome and FMS and evaluating possible treatment options.Publication Metadata only Eksüdatif yaşa bağlı makula dejenerasyonunun etiyopatogenezinin aydınlatılması amacıyla ARPE-19 hücre hattında CoCl2 ile indüklenen hipoksi modeli oluşturulması(2021-10-31T00:00:00Z) Süsgün, Seda; Korkmaz, Nur Damla; Kutlutürk, Işıl; Akbaş, Fahri; Özdemir, Mehmet Hakan; Elbay, Ahmet; SÜSGÜN, SEDA; KORKMAZ, NUR DAMLA; AKBAŞ, FAHRİ; ÖZDEMİR, MEHMET HAKAN; ELBAY, AHMET