Person: SÜSGÜN, SEDA
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Publication Metadata only Calcium homeostasis in cisplatin resistant epithelial ovarian cancer.(2019-07-01) Kucukkaya, B; Basoglu, H; Erdag, D; Akbas, FAHRİ; Yalcintepe, L; AKBAŞ, FAHRİ; SÜSGÜN, SEDAIntracellular calcium concentration ([Ca2+](i)) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high success rate in cancer therapies. We investigated the regulatory role of [Ca2+]in cisplatin resistance in epithelial ovarian cancer cell line, in MDAH-2774, and its chemoresistant subclone MDAH-2774/DDP. The measurement of [Ca2+](i) using fluorescence microscope, and flow cytometry revealed that the amount of intracellular calcium decreased in cisplatin resistant cells compared to the amounts in parental cells. mRNA expression profiles of calcium homeostasis-associated major genes (IP(3)R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) decreased in cisplatin resistant cell line in comparison to the expression profiles in parental cells. Owing to the changes in the expression of genes involved in calcium regulation, these results show, drug resistance may be prevented by introducing a new perspective on the use of inhibitors and activators of these genes, and thus of cytostatic treatment strategies, due to changes in the expression of genes involved in calcium regulation.Publication Metadata only Possible effects of caffeic acid phenethyl ester and thymoquinone against toluene brain injury(2019-08-31T00:00:00Z) MEYDAN, SEDAT; EŞREFOĞLU, MUKADDES; SELEK, ŞAHBETTİN; AKBAŞ TOSUNOĞLU, ESRA; öztürk, özlem; kurbetli, nuriye; BAYINDIR, NİHAN; BULUT, HURİ; MERAL, İSMAİL; SÜSGÜN, SEDA; AKBAŞ, FAHRİ; MEYDAN, SEDAT; EŞREFOĞLU, MUKADDES; SELEK, ŞAHABETTİN; MERAL, İSMAİL; SÜSGÜN, SEDA; AKBAŞ, FAHRİPublication Metadata only Effects of Methylprednisolone in the Treatment of Spinal Cord Injuries by Evaluation of microRNA-21: An Experimental Study.(2022-04-19T00:00:00Z) Abdallah, Anas; Tekin, Abdurrahim; Oztanir, Mustafa Namik; Süsgün, Seda; Yabacı, AYŞEGÜL; Çınar, İrfan; Can, Engin; Tokar, Sadık; Akbaş, Fahri; Seyithanoğlu, Mehmet Hakan; SÜSGÜN, SEDA; YABACI TAK, AYŞEGÜL; AKBAŞ, FAHRİ; SEYİTHANOĞLU, MEHMET HAKANPublication Metadata only Fibromiyalji Sendromu ile Bağırsak Mikrobiyotası Arasındaki İlişkinin Araştırılması(2020-12-31T00:00:00Z) Albayrak, Büşra; Süsgün, Seda; Küçükakkaş, Okan; Akbaş, Fahri; Yabacı, Ayşegül; Özçelik, Semra; SÜSGÜN, SEDA; KÜÇÜKAKKAŞ, OKAN; AKBAŞ, FAHRİ; YABACI TAK, AYŞEGÜLFibromyalgia syndrome (FMS) is one of the frequent forms of chronic widespread pain, with a reported prevalence of 2% to 4% in the adult population. Nervous system dysfunction is implicated in FMS and neurotransmitters are the targets of a number of drugs approved for fibromyalgia. However exact underlying mechanisms of FMS are unknown yet and multiple hypotheses being suggested. Considering the relation between fibromyalgia and irritable bowel syndrome, altered gut microbiome could be associated with fibromyalgia. Herein, altered gut microbiome levels were investigated in patients with FMS compared to healthy controls. We collected faeces to study the microbiome, from a cohort of 54 patients with FMS and 36 healthy individuals. Extraction of DNA was carried out with commercial kit according to the manufacturer’s recommendations. Samples were compared using 16S rRNA gene amplification with specific primers of Bacteroidetes, Firmicutes, Enterobacteria, Lactobacillus, Streptococcus and Bifidobacterium by the Real-Time PCR instrument. According to our results, Bacteroidetes and Bifidobacteria were increased statistically significant (p<0.05), Firmicutes was decreased (p<0.001) in the patient group. No statistically significant results were found for Enterobacterium, Streptococcus and Lactobacillus (p>0.05). When the relationship between bacteria was evaluated, statistically significant and negative correlation was found between Bacteroidetes and Firmicutes percentage (r =-0.778, p <0.001). A moderately positive correlation was observed between the percentage of Enterobacter and Bifidobacter (r = 0.460, p = 0.005). The results suggest that the gut microbiota may play a role in fibromyalgia.t the gut microbiota may play a role in fibromyalgia. In sum, it is clear that large-scale further research in larger cohorts will be efficient in understanding the relationship of gut microbiome and FMS and evaluating possible treatment options.Publication Metadata only Eksüdatif yaşa bağlı makula dejenerasyonunun etiyopatogenezinin aydınlatılması amacıyla ARPE-19 hücre hattında CoCl2 ile indüklenen hipoksi modeli oluşturulması(2021-10-31T00:00:00Z) Süsgün, Seda; Korkmaz, Nur Damla; Kutlutürk, Işıl; Akbaş, Fahri; Özdemir, Mehmet Hakan; Elbay, Ahmet; SÜSGÜN, SEDA; KORKMAZ, NUR DAMLA; AKBAŞ, FAHRİ; ÖZDEMİR, MEHMET HAKAN; ELBAY, AHMET