Person: SELEK, ŞAHABETTİN
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Publication Kardiyovasküler Hastalıklarda Biyotinidaz ve TCA Enzim Aktivitelerinin İncelenmesi(2023-07-09) Sarıkaya U.; Çimen Y. A.; Taşlıdere B.; Selek Ş.; Açıkgöz N.; Meral İ.; SARIKAYA, UFUK; ÇİMEN, YASİN ALİ; TAŞLIDERE, BAHADIR; SELEK, ŞAHABETTİN; AÇIKGÖZ, NUSRET; MERAL, İSMAİLPublication Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters(2012-09-01T00:00:00Z) Erturk, Cemil; Altay, Mehmet Akif; Selek, Sahbettin; Kocyigit, ABDÜRRAHİM; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİMBackground. The aim of this study was to investigate serum paraoxonase-1 (PON1) activity and oxidative/anti-oxidative status in knee osteoarthritis (OA), and evaluate their relationship using radiological and clinical parameters. Materials and methods. The study population comprised 127 patients with knee OA and 107 healthy volunteers. Patients with knee OA were divided into four subgroups according to the Kellgren-Lawrence (K&L) grading scale. In addition, each patient was clinically evaluated by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Serum PON1 activity was measured spectrophotometrically. Oxidative status was assessed by measuring serum lipid hydroperoxide (LOOH) and total oxidant status (TOS). Anti-oxidative status was assessed by measuring serum free sulfydryl groups (-SH = total thiol) and total antioxidant capacity (TAC). Oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods. Results. Serum PON1 activity was significantly lower in the knee OA group compared to the control group (p 0.05). The lowest and highest mean serum PON1 activities were detected in patients with grades 4 and 1, respectively (ANOVA p < 0.001). In multiple regression analysis, WOMAC score was independently associated with serum PON1 activity (beta = -0.248, p = 0.027). Conclusions. Decreased serum PON1 activity and elevated LOOH, TOS, and OSI levels may be associated with knee OA, and serum PON1 activity may be a useful adjunctive indicator of the severity of knee OA for follow-up.Publication Serum paraoxonase enzyme activity and oxidative stress in obese subjects(2011-01-01T00:00:00Z) ASLAN, Mehmet; Horoz, Mehmet; Sabuncu, Tevfik; Celik, Hakim; Selek, Sahbettin; SELEK, ŞAHABETTİNIntroduction Obesity is an important risk factor for atherosclerotic cardiovascular disease. Paraoxonase-1 (PON1) may play a significant role in the prevention of obesity-related accelerated atherosclerosis by hydrolyzing lipid peroxides in oxidized low-density lipoproteins.Publication MicroRNA expressions are altered in endometrial tissues of PCOS women(2017-09-01) BULUT, HURİ; BULUT, Berk; SERDARZADE, A.; Yuksel, B.; Selek, S.; BULUT, HURI; SELEK, ŞAHABETTİNPublication Hipotroidili hastalarda paraoxonase aktivitesinin ölçülmesi ve ateroskleroz ile ilişkisinin araştırılması(2018-05-11T00:00:00Z) ÇELİK, HAKİM; Aksoy, Nurten; SELEK, ŞAHBETTİN; EREL, ÖZCAN; SELEK, ŞAHABETTİNPublication Farelede Radyoterapinin Etkileri(2015-04-27T00:00:00Z) TAŞPINAR, ÖZGÜR; AYDIN, TEOMAN; KILIÇ, GÖKHAN; EREN, FATMA; KIZILTAN, HURİYE; KEPEKÇİ, MÜGE; ERİŞ, HİKMET; KESKİN, YAŞAR; ÖZER, ÖMER FARUK; SELEK, ŞAHBETTİN; SEYİTHANOĞLU, MEHMET HAKAN; AYDIN, TEOMAN; KILIC, GÖKHAN; KESKİN, YAŞAR; ÖZER, ÖMER FARUK; SELEK, ŞAHABETTİNPublication Protective effects of caffeic acid phenethyl ester and thymoquinone on toluene induced liver toxicity.(2019-05-01) Esrefoglu, MUKADDES; Bayındır, NİHAN; KURBETLI, N; Selek, S; Akbas, Tosunoglu; Meydan, SEDAT; OZTURK, OSMAN; Bulut, HURİ; Meral, I; MEYDAN, SEDAT; EŞREFOĞLU, MUKADDES; SELEK, ŞAHABETTİN; ÖZTÜRK, OSMAN; BAYINDIR, NİHAN; BULUT, HURI; MERAL, İSMAİLPublication METABOLOMIC PROFILING IN DISTINCT TYPES OF LEUKEMIA(2024-02-29) Gül A. Z.; Selek Ş.; Bekiroğlu S.; Demirel M.; Çakır F. B.; Uyanık B.; SELEK, ŞAHABETTİN; DEMİREL, METİNPublication Molecular cloning and characterization of NAD(+) dependent isocitrate dehydrogenase enzyme from Shewanella putrefaciens(2021-10-01T00:00:00Z) Fahri, Akbas; Metin, Demirel; Ahmet, Ozaydin; Sahabettin, ŞAHBETTİN; AKBAŞ, FAHRİ; DEMİREL, METİN; SELEK, ŞAHABETTİNIsocitrate dehydrogenase (IDH) is a fundamental enzyme for carbon metabolism in the Krebs cycle. This enzyme is required for oxidation-reduction reactions in both eukaryotic and prokaryotic cells and plays a critical role in their growth and pathogenesis. In this study, we cloned the gene encoding NAD(+) dependent isocitrate dehydrogenase from Shewanella putrefaciens. The expression of recombinant protein was induced with 0.5 mM of IPTG. His-tagged IDH overexpressed in E. coli was purified and characterized. The expressed IDH enzyme was purified in an active soluble form. The molecular weight of the enzyme was confirmed with Western blotting. High sequence homology was observed with IDH sequences of other Shewanella strains and remarkable sequence homology was found with other bacteria reported in the database.Publication Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis(2018-01-01) Koyuncu, Ismail; Kocyigit, ABDÜRRAHİM; Ozer, Ahmet; SELEK, Sahabettin; Kirmit, Adnan; Karsen, Hasan; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİNSerologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humans