Person: AYŞAN, MUSTAFA ERHAN
53 results
Search Results
Now showing 1 - 10 of 53
Publication Open Access Parathyroid allotransplantation in rabbits without cultivation.(2014-01-15) CAN, I; YUCESAN, E; Aysan, E; SAYITOGLU, M; OZBEK, U; ERCIVAN, M; ATASOY, H; BUYUKPINARBASILI, NUR; MUSLUMANOGLU, M; AYŞAN, MUSTAFA ERHAN; YÜCESAN, EMRAH; BÜYÜKPINARBAŞILI, NURPermanent hypoparathyroidism is a serious clinical situation. Allotransplantation of the parathyroid cells is relatively new approach to treatment. Non-cultivated allotransplantation in rabbits is not tried before. In this research parathyroidectomy was performed in six female New Zealand white rabbits. After division of surgically removed tissues into two, cryopreservation after cell isolation was done. Non-cultivated cross allotransplantation was performed under immunosuppression. Serum calcium and phosphorus levels were observed 15 days and histopathological analyses of the transplanted parathyroid tissues were studied. Significant changes in serum calcium and phosphorus levels during the experiment were observed (p=0.001 for both). Calcium levels which were significantly dropped to 6.66±0.7 mg/dL after parathyroidectomy and progressively increased up to 15.98±1.25 mg/dL at the end of the experiment (p=0.004). Phosphorus levels which were increased to 9.38±0.63 mg/dL after parathyroidectomy and stabilized to 4.46±1.06 mg/dL at the end of the experiment (p=0.007). All allotransplanted parathyroid tissues showed normal tissue architecture without evidence of cellular rejection. In conclusion allotransplantation of the parathyroid tissues without cultivation may be considered as an alternative and safe approach for the treatment of permanent hypoparathyroidism.Publication Metadata only A NOVEL HERBAL EXTRACT MEDICINE FOR LIVER HEMOSTASIS:AN EXPERIMENTAL ANIMAL STUDY(2019-03-01) KUNDUZ, ENVER; DİNCEL, Zeyneb Afra; ŞEKER, Büşra; SAYLAK, HATİCE KÜBRA; DEMİR, Okan; Akbulut, HÜSEYİN; Ayşan, MUSTAFA ERHAN; KUNDUZ, ENVER; AKBULUT, HÜSEYİN; AYŞAN, MUSTAFA ERHANPublication Metadata only Effects of boric acid-linked ampicillin on the rat intra-abdominal sepsis model(2019-03-01T00:00:00Z) Idiz, Ufuk Oguz; Aysan, Erhan; Firat, Deniz; ERCAN, ÇİLEM; Demirci, Selami; Sahin, Fikrettin; AYŞAN, MUSTAFA ERHAN; ERCAN, ÇİLEMThe aim of this study was to determine efficiency of a new molecule that was obtained by linking boric acid with ampicillin in treating intra-abdominal infection. Following intraperitoneal E. coli injection totwenty-one female Wistar albino rats, group 1 was administered boron-linked ampicillin, group 2 was administered only ampicillin and group 3 was injected intraperitoneally with physiological serum. IL-6, and a white blood cell analysis was performed from the blood before and on the seventh day of treatment. No statistically significant difference in blood WBC levels after treatment was found among the groups. There was no statistically significant difference in the IL-6 values of group 2 and group 3 before and after the treatment (p=0.195 and 0.193, respectively); however, the reduction in the serum IL-6 values of group 1 was statistically significant (p=0.003). Boric acid-linked ampicillin is a more effective intra-abdominal infection treatment compared with ampicillin alone.Publication Open Access Efficacy of glycerol and flax seed oil as anti-adhesive barriers after thyroidectomy.(2014-06-28) IDIZ, O; Aysan, E; FIRAT, D; ERSOY, YELİZ EMİNE; CENGIZ, MB; AKBULUT, H; ISıK, A; MUSLUMANOGLU, M; AYŞAN, MUSTAFA ERHAN; ERSOY, YELIZ EMINEWe evaluated the effects of local flax seed oil and glycerol application for reducing adhesion formation after thyroidectomy. Material/Method: We randomly assigned 18 female Wistar albino rats (median weight: 275 g, median age: 4.5 mth) to 3 groups: 0.1 ml 0.9% NaCl, glycerol, and flax seed oil were sprayed in a perithyroidal area after thyroidectomy operation on all animals as anti-adhesive barriers. Rats were sacrificed on the postoperative 14th day and adhesions were evaluated macroscopically and microscopically. Results: The median macroscopic adhesion score was 3.0±0.0 in the 0.9% NaCl group, 1.33±0.52 in the glycerol group, and 1.67±0.53 in the flax seed oil group. The median histopathological fibrosis scores were 2.33±0.82, 0.67±0.52, and 0.83±0.75, respectively. Both glycerol and flaxseed oil group macroscopic and microscopic scores were significantly lower than the 0.9% NaCl group (p<0.05). There was no significant difference among the groups (p>0.05). Conclusions: Glycerol and flax seed oil both decrease the incidence of post-thyroidectomy adhesion in rats, but glycerol is more effective.Publication Metadata only Mikroenkapsüle edilen paratiroid hücrelerinin in-vitro optimizasyonu(2017-12-01) YUCESAN, Emrah; GONCU, Beyza; BAŞOĞLU, HARUN; ÖZTEN KANDAŞ, NUR; ERSOY, YELİZ EMİNE; AKBAŞ, FAHRİ; AYŞAN, MUSTAFA ERHAN; BAŞOĞLU, HARUN; GÖNCÜ, BEYZA SERVET; ÖZTEN KANDAŞ, NUR; ERSOY, YELIZ EMINE; AKBAŞ, FAHRİ; AYŞAN, MUSTAFA ERHANPublication Metadata only NEW TRANSPORT SOLUTION FOR PARATHYROIDALLOTRANSPLANTATION (FR) (NFR)(2017-09-27) GONCU, Beyza; OZDEMIR, Burcu; BAŞOĞLU, HARUN; ÖZTEN KANDAŞ, NUR; AKBAŞ, FAHRİ; KESKİN TOKA, Cemile; YUCESAN, Emrah; KAZANCIOĞLU, RÜMEYZA; AYŞAN, MUSTAFA ERHAN; GÖNCÜ, BEYZA SERVET; BAŞOĞLU, HARUN; ÖZTEN KANDAŞ, NUR; AKBAŞ, FAHRİ; KAZANCIOĞLU, RÜMEYZA; AYŞAN, MUSTAFA ERHANPublication Metadata only . Paratiroid Doku Transplantasyonu, Türkiye’de Bir İlk. Hayata Bağış Dergisi(2014-03-01) AYŞAN, MUSTAFA ERHAN; gök, özlem; ALTUĞ, BURCUGÜL; ERCAN, ÇİLEM; AYŞAN, MUSTAFA ERHAN; ERCAN, ÇİLEMPublication Open Access Safe thyroidectomy with intraoperative methylene blue spraying.(2012-11-13) SARI, S; Aysan, E; MUSLUMANOGLU, M; ERSOY, YELİZ EMİNE; BEKTASOGLU, HÜSEYİN KAZIM; YARDıMCı, ERKAN; AYŞAN, MUSTAFA ERHAN; ERSOY, YELIZ EMINE; BEKTAŞOĞLU, HÜSEYİN KAZIM; YARDIMCI, ERKANBackground: We aimed to minimalize operative complications by spraying of methylene blue stain on thyroid glands and the perithyroidal area. Material and methods: The intra-operative methylene blue spraying technique was used prospectively on a total of 56 patients who had undergone primary (not recurrent) thyroid surgery for a variety of thyroid diseases. Bilateral total thyroidectomy was performed in all cases. After superior but before inferior pole ligation, 0.5ml of methylene blue was sprayed over the thyroid lobe and perilober area. Tissues, especially parathyroides, the recurrent laryngeal nerve, and the inferior thyroid artery, were identified and evaluated. Results: Recurrent laryngeal nerve and arteries were not stained and thus they remained white in all cases while all other tissues were stained blue. Within three minutes parathyroid glands washed out the blue stain and the original yellow color was regained. Thyroid tissue wash-out time was not less than 15 minutes; perithyroideal muscles, tendinous and lipoid structures took no less than 25 minutes. Conclusion: The safety of intravascular methylene blue guidance on thyroid surgery is known. This research demonstrates the effectiveness of the spraying technique, a new technique which ensures not only identification of parathyroid glands within three minutes, but also identification of recurrent laryngeal nerves and inferior thyroid arteries.Publication Metadata only Does adrenaline spraying over thyroidectomy area reduce bleeding?(2014-01-01) AYSAN, Erhan; MERIC, Aysenur; Kadioglu, HÜSEYİN; CENGIZ, Merve B.; Bozkurt, SÜLEYMAN; Memmi, NAİM; CIPE, Gokhan; MUSLUMANOGLU, Mahmut; ERSOY, YELIZ EMINE; AYŞAN, MUSTAFA ERHAN; MERİÇ HAFIZ, AYŞENUR; KADIOĞLU, HÜSEYİN; BOZKURT, SÜLEYMAN; MEMMİ, NAİMBackground: Means to prevent and control intra-or postoperative bleeding remain a topic of utmost importance in thyroidectomy. In this randomised clinical trial, we used adrenaline spraying to see if it helps bleeding control and reduces drainage and hematoma formation after thyroidectomy. Methods: After total thyroidectomy, 1 mg/ml adrenaline solution in 10 ml saline was sprayed all over the operation area by a syringe in 40 patients of -Adrenaline (+) Group-. In the other 40 patients in -Adrenaline (-) Group-, only standart total thyroidectomy was performed. Drainage amounts of 24 hours were recorded. Results: Among 80 patients, 66 (82.5%) were female and 14 (17.5%) were male. The daily drainage amounts of the Adrenaline (+) Group were found statistically significantly lower than the Adrenaline (-) Group (p<0.05). In both of the groups, thyroid volumes were significantly correlated with the drainage amounts. -p- values were 0.008 and <0.001 in Adrenaline (+) and Adrenaline (-) Groups, respectively. Conclusions: Preliminary experience using adrenaline has been encouraging and it is useful as an adjunct to thyroid surgery in order to prevent hemorrhagia and give up drain placement. But prospective randomized trials using adequate patient numbers are still needed to validate efficacy and safety.Publication Metadata only Parathyroid Allotransplant for Persistent Hypocalcaemia: A New Technique Involving Short-Term Culture. Experimental and clinical transplantation(2016-11-01) KILIÇ, ÜLKAN; AYŞAN, MUSTAFA ERHAN; gök, özlem; ALTUĞ, BURCUGÜL; ERCAN, ÇİLEM; İDİZ, UFUK OĞUZ; AYŞAN, MUSTAFA ERHAN; ERCAN, ÇİLEM