Person: TEKKELİ, ŞERİFE EVRİM
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Publication Metadata only ANALYTICAL METHODS FOR THE DETERMINATION OF POLYBROMINATED DIPHENYL ETHERS IN HUMAN MILK(2020-03-01T00:00:00Z) ASRAM SAĞIROĞLU, ALİ; Onal, Cem; DİNCEL, DEMET; Onal, Armagan; ASRAM SAĞIROĞLU, ALİ; TEKKELİ, ŞERİFE EVRİM; DİNCEL, DEMETPolybrominated diphenyl ethers (PBDEs), which are used as flame retardants, are widely used additives so many different kind of materials that are consumed by public. Current researches reveal a significant increase in the levels of PBDEs in human biological fluids all around the world. According to International Agency for Research on Cancer (IARC), PBDEs are declarated as carcinogenic agents and additionally a considerable risks have been shown in animal studies such as liver damage, alteration of thyroid hormone levels, neurotoxicity and hazardous effects on development of fetus. Due to the simplicity of human exposure routes such as ingestion, inhalation or dermal contact, it gains high importance to quantify PBDEs in biological fluids. The exposure to PBDEs is critic aspecially during pregnancy, fetus development and infancy. Excretion of PBDEs in human milk is the focus area for researchers in recent years.Publication Metadata only SIMULTANEOUS DETERMINATION OF FEBUXOSTAT AND MONTELUKAST IN HUMAN PLASMA BY USING FABRIC PHASE SORPTIVE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(2021-09-17T00:00:00Z) GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİM; Kabir, Abuzar; Aslan, Ceylin; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİMPublication Open Access Effects of prebiotic oligofructose-enriched inulin on gut-derived uremic toxins and disease progression in rats with adenine-induced chronic kidney disease(2021-10-06T00:00:00Z) Cetinkaya, M. Alper; Kepekci-Tekkeli, Şerife Evrim; Kul, Oguz; Samur, Gulhan; TEKKELİ, ŞERİFE EVRİMRecent studies suggest that dysbiosis in chronic kidney disease (CKD) increases gut-derived uremic toxins (GDUT) generation, leads to systemic inflammation, reactive oxygen species generation, and poor prognosis. This study aimed to investigate the effect of oligofructose-enriched inulin supplementation on GDUT levels, inflammatory and antioxidant parameters, renal damage, and intestinal barrier function in adenine-induced CKD rats. Male Sprague-Dawley rats were divided into control group (CTL, n = 12) fed with standard diet; and CKD group (n = 16) given adenine (200 mg/kg/day) by oral gavage for 3-weeks to induce CKD. At the 4th week, CKD rats were subdivided into prebiotic supplementation (5g/kg/day) for four consecutive weeks (CKD-Pre, n = 8). Also, the control group was subdivided into two subgroups; prebiotic supplemented (CTL-Pre, n = 6) and non-supplemented group (CTL, n = 6). Results showed that prebiotic oligofructose-enriched inulin supplementation did not significantly reduce serum indoxyl sulfate (IS) but did significantly reduce serum p-Cresyl sulfate (PCS) (p = 0.002) in CKD rats. Prebiotic supplementation also reduced serum urea (p = 0.008) and interleukin (IL)-6 levels (p = 0.001), ameliorated renal injury, and enhanced antioxidant enzyme activity of glutathione peroxidase (GPx) (p = 0.002) and superoxide dismutase (SOD) (p = 0.001) in renal tissues of CKD rats. No significant changes were observed in colonic epithelial tight junction proteins claudin-1 and occludin in the CKD-Pre group. In adenine-induced CKD rats, oligofructose-enriched inulin supplementation resulted in a reduction in serum urea and PCS levels, enhancement of the antioxidant activity in the renal tissues, and retardation of the disease progression.Publication Metadata only Çocuklarda Dikkat Eksikliği Hiperaktivite Bozukluğu Durumunda Kullanılan İlaçlar, Gıda Takviyeleri ve Fitofarmasötiklerin İncelenmesi(2021-11-17T00:00:00Z) Tekkeli, Şerife Evrim; Özbek, Emirhan; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİMPublication Metadata only Development of An HPLC Method for the Determination of Mesalazine in Human Plasma by Fluorimetric Derivatization and Application to A Prototype Pharmacokinetic Study(2021-11-01T00:00:00Z) Ceylan, Burhan; Tekkeli, Evrim Kepekci; Onal, Cem; TEKKELİ, ŞERİFE EVRİMIn this study, a new, fast and sensitive HPLC method with fluorometric detection was developed for the determination of mesalazine in human plasma and applied to a pharmacokinetic study. Mesalazine was precolumn derivatized with NBD-Cl and the fluorescent derivative was separated on a C18 (150 x 4.6 mm x 2.6 mu m) analytical column at 30 oC using a mobile phase composed of acetonitrile-0.1% o-phosphoric acid in water (70:30, v/v) by isocratic elution with flow rate of 1.0 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection (lambda(ex) = 280 nm, lambda(em) = 325 nm). The retention time of mesalazine is 3.08 +/- 0.06 min. Nortriptiline was used as internal standard. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 0.25-1.5 mu g mL(-1) with the correlation coefficient of 0.9997. LOD and LOQ were found to be 0.075 and 0.25 mu g mL(-1), respectively. Intraday and interday RSD values were less than 5.92%. The plasma concentration-time profile and pharmacokinetic parameters such as AUC(0-t), AUC(0-infinity), C-max, t(max), t(1/2), were calculated according to the assays. The presented method can certainly be used for bioequivalence and bioavailability investigations and routine analysis of the drug in plasma.Publication Metadata only Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent(2020-01-01T00:00:00Z) TEKKELİ, ŞERİFE EVRİM; TEKKELİ, ŞERİFE EVRİMA sensitive method is presented for the determination of the ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride). Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate (pH 10) to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0-200.0 ng mL(-1), with a coefficient of determination of 0.9999 with a limit of detection (LOD) of 0.14 ng mL(-1) and the limit of quantitation (LOQ) of 0.46 ng mL(-1). The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.Publication Metadata only A new HPLC method for the determination of Allicin and S-Allyl cysteine in garlic (Allium sativum L.) extracts(2020-09-01T00:00:00Z) BUCAK, Seden; TEKKELİ, ŞERİFE EVRİM; CEYLAN, BURHAN; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİM; TIRIS, GİZEMPublication Metadata only Liquid chromatographic analysis for the determination of deferasirox in pharmaceutical formulations and spiked plasma samples using dansyl chloride reagent(2020-01-01T00:00:00Z) Asram Sağıroğlu, Ali; ASRAM SAĞIROĞLU, ALİ; TEKKELİ, ŞERİFE EVRİMPublication Metadata only Development of sol-gel phenyl/methyl/poly (dimethylsiloxane) sorbent coating for fabric phase sorptive extraction and its application in monitoring human exposure to selected polycyclic aromatic hydrocarbons using high performance liquid chromatography-fluorescence detection(2021-01-01T00:00:00Z) GAZİOĞLU, IŞIL; Kabir, Abuzar; Zengin, Ozge S.; Tekkeli, Evrim Kepekci; Furton, Kenneth G.; Tartaglia, Angela; Locatelli, Marcello; GAZİOĞLU, IŞIL; TEKKELİ, ŞERİFE EVRİMFollowing the convenient, yet very powerful pathway to create designer extraction sorbent using sol-gel chemistry, a novel sol-gel phenyl/methyl/poly(dimethylsiloxane) sorbent coating was created on polyester fabric substrate for fabric phase sorptive extraction (FPSE) and was subsequently applied to monitor human exposure to selected polycyclic aromatic hydrocarbons (PAHs) including pyrene, chrysene, and benzo[a]pyrene in plasma samples obtained from tobacco smoker volunteers using high performance liquid chromatography fluorescence detector (HPLC-FLD). A rapid FPSE-HPLC-FLD method was developed that adequately resolved the PAHs chromatographically, after their successful extraction from human plasma using fabric phase absorption extraction (FPSE) and subsequently analysed in the liquid chromatographic system by means of an analytical column (InterSustain C-18 column 150 x 4.6 mm, 5 mu m) using acetonitrile (ACN) and water as mobile phases in gradient elution mode. With the optimized conditions, the retention times were found to be 6.168, 7.214, and 10.404 min for pyrene, chrysene, and benzo[a]pyrene, respectively. The total chromatographic runtime was limited to 12.5 min.Publication Metadata only Evaluation of Monomer Release of Bulk Fill Composite Resins with Extended Light Curing(2022-04-22T00:00:00Z) Tırıs, Gizem; Dönmez, Nazmiye; Dalkılıç, Evrim; ÖZDUMAN, ZÜMRÜT CEREN; TIRIS, GİZEM; TEKKELİ, ŞERİFE EVRİM; DÖNMEZ, NAZMİYE; DALKILIÇ, EVRIM