Person:
ÇETİN, NESİBE SELMA

Profile Picture
Status
Organizational Units
OrgUnit Logo
Organizational Unit
Job Title
First Name
NESİBE SELMA
Last Name
ÇETİN
Name
Email Address
Birth Date

Filters

2021 - 20235
Reset filters

Settings

Rights: info:eu-repo/semantics/openAccess ×

Search Results

Now showing 1 - 5 of 5
  • Thumbnail Image
    PublicationOpen Access
    Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
    (2021-12-01T00:00:00Z) Karaaslan, Elif; Kalkan-Yazıcı, Merve; Hasanoğlu, Sevde; Karakeçili, Faruk; Özdarendeli, Aykut; Kalkan, Ahmet; Kılıç, Ali Osman; Doymaz, Mehmet Ziya; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYA
    In 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations.
  • No Thumbnail Available
    PublicationMetadata only
    The Utility of CRISPR/Cas9 Technology to Attenuate Apoptotic Pressure in Monoclonal Antibody (mAb) Producing Cells
    (2022-10-20) Doymaz M. Z.; Aydın B. N.; Çetin N. S.; Kaygusuz A.; DOYMAZ, MEHMET ZIYA; ÇETİN, NESİBE SELMA
  • No Thumbnail Available
    PublicationMetadata only
    COVID-19 ENFEKSİYONUN MİKROBİYOLOJİSİ
    (Bezmialem Vakıf Üniversitesi Yayınları, 2021-01-01) DOYMAZ M. Z.; SÜMBÜL B.; AKBAŞ E.; KARAASLAN E.; ÇETİN N. S.; YAZICI M.; DOYMAZ, MEHMET ZIYA; SÜMBÜL, BİLGE; ÇETİN, NESİBE SELMA; YAZICI, MERVE
  • Thumbnail Image
    PublicationOpen Access
    Cross-Reactive anti-Nucleocapsid Protein Immunity against Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus in Multiple Species.
    (2021-01-13T00:00:00Z) Kalkan-Yazıcı, Merve; Karaaslan, Elif; Çetin, Nesibe Selma; Hasanoğlu, Sevde; Güney, Filiz; Zeybek, Ümit; Doymaz, Mehmet Ziya; YAZICI, MERVE; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYA
    The World Health Organization estimates that there may be three billion people at risk of infection by Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a highly lethal, emerging orthonairovirus carried by ticks. On the other hand, the closely related Hazara virus (HAZV), a member of the same serogroup, has not been reported as a pathogen for humans. Given the structural and phylogenetic similarities between these two viruses, we evaluated the immunological similarities of the nucleocapsid protein (NP) of these two viruses in multiple species. Strong antigenic similarities were demonstrated in anti-NP humoral immune responses against HAZV and CCHFV in multiple species using convalescent human CCHF sera, rabbit and mouse polyclonal antiserum raised against CCHFV, and mouse polyclonal antiserum against CCHFV-NP in enzyme immunoassays. We also report a convincing cross-reactivity between NPs in Western blots using HAZV-infected cell lysate as antigen and inactivated CCHFV and CCHFV-NP-immunized mice sera. These results suggest that NPs of HAZV and CCHFV share significant similarities in humoral responses across species and underline the potential utility of HAZV as a surrogate model for CCHFV.IMPORTANCE CCHFV and HAZV, members of the Nairoviridae family, are transmitted to mammals by tick bites. CCHFV is considered to be a severe threat to public health and causes hemorrhagic diseases with a high mortality rate, and there are neither preventative nor therapeutic medications against CCHFV disease. HAZV, on the other hand, is not a pathogen to humans and can be studied under BSL-2 conditions. The antigenic relationship between these viruses is of interest for vaccines and for preventative investigations. Here, we demonstrate cross-reactivity in anti-NP humoral immune response between NPs of HAZV and CCHFV in multiple species. These results underline the utility of HAZV as a surrogate model to study CCHFV infection.
  • No Thumbnail Available
    PublicationMetadata only
    STABLE EXPRESSION OF PROTEINS OF CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS (CCHFV) IN EUKARYOTIC CELLS: A RELIABLE MODEL FOR INVESTIGATING VIRUS BIOLOGY AND PATHOGENESIS
    (2023-09-19) Bakangil Ö.; Yazıcı M.; Çetin N. S.; Doymaz M. Z.; BAKANGİL, ÖZLEM; YAZICI, MERVE; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYA