Person: ÇETİN, NESİBE SELMA
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Publication Open Access Discordance between Serum Neutralizing Antibody Titers and the Recovery from COVID-19(2020-09-25T00:00:00Z) Koç, Mm; Kalkan, Yazıcı; Çetin, Nesibe Selma; Doymaz, Mz; Sümbül, B; Durdu, B; YAZICI, MERVE; MERİÇ KOÇ, MELİHA; ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; OKAY, GÜLAY; DURDU, BÜLENT; SÜMBÜL, BİLGE; DOYMAZ, MEHMET ZIYAThe recent pandemic of COVID-19 has caused a tremendous alarm around the world. Details of the infection process in the host have significant bearings on both recovery from the disease and on the correlates of the protection from the future exposures. One of these factors is the presence and titers of neutralizing Abs (NAbs) in infected people. In the current study, we set out to investigate NAbs in the recovered subjects discharged from the hospital in full health. Serum samples from a total of 49 documented consecutive COVID-19 subjects were included in the study. All the subjects were adults, and serum samples collected during the discharge were tested in viral neutralization, enzyme immunoassay (EIA), and Western immunoblot tests against viral Ags. Even though a majority of the recovered subjects had raised significant NAb titers, there is a substantial number of recovered patients (10 out of 49) with no or low titers of NAbs against the virus. In these cohorts as well as in patients with high NAb titers, viral Ag binding Abs were detectable in EIA tests. Both NAb titers and EIA detectable Abs are increased in patients experiencing a severe form of the disease, and in older patients the Ab titers were heightened. The main conclusion is that the recovery from SARS-CoV-2 infection is not solely dependent on high NAb titers in affected subjects, and this recovery process is probably produced by a complex interplay between many factors, including immune response, age of the subjects, and viral pathology.Publication Metadata only Evaluation of CCHFV nucleoprotein as diagnostic tool in ELISA(2017-09-10) ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; YAZICI, MERVE; Hasanoğlu, Sevde; KALKAN, AHMET; KILIÇ, ALİ OSMAN; ÖZDARENDELİ, AYKUT; DOYMAZ, MEHMET ZİYA; ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; YAZICI, MERVE; DOYMAZ, MEHMET ZIYAPublication Open Access Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)(2021-12-01T00:00:00Z) Karaaslan, Elif; Kalkan-Yazıcı, Merve; Hasanoğlu, Sevde; Karakeçili, Faruk; Özdarendeli, Aykut; Kalkan, Ahmet; Kılıç, Ali Osman; Doymaz, Mehmet Ziya; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYAIn 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations.Publication Metadata only Investigation Of The Cellular And Humoral Immune Responses Against Nucleoprotein Of Crimean Congo Hemorrhagic Fever Virus (CCHFV)(2019-05-10) ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; DOYMAZ, MEHMET ZİYA; KILIÇ, ALİ OSMAN; ÇETİN, NESİBE SELMA; KARAASLAN, ELİF; DOYMAZ, MEHMET ZIYAPublication Metadata only INVESTIGATION OF HUMORAL RESPONSE RAISED AGAINST BACTERIALLY EXPRESSED NUCLEOPROTEIN OF CRIMEAN CONGO HEMORRHAGIC FEVER VIRUS(2016-04-27) KARAASLAN, ELİF; ÇETİN, NESİBE SELMA; KILIÇ, ALİ OSMAN; DOYMAZ, MEHMET ZİYA; KARAASLAN, ELİF; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYAPublication Metadata only The Utility of CRISPR/Cas9 Technology to Attenuate Apoptotic Pressure in Monoclonal Antibody (mAb) Producing Cells(2022-10-20) Doymaz M. Z.; Aydın B. N.; Çetin N. S.; Kaygusuz A.; DOYMAZ, MEHMET ZIYA; ÇETİN, NESİBE SELMAPublication Metadata only COVID-19 ENFEKSİYONUN MİKROBİYOLOJİSİ(Bezmialem Vakıf Üniversitesi Yayınları, 2021-01-01) DOYMAZ M. Z.; SÜMBÜL B.; AKBAŞ E.; KARAASLAN E.; ÇETİN N. S.; YAZICI M.; DOYMAZ, MEHMET ZIYA; SÜMBÜL, BİLGE; ÇETİN, NESİBE SELMA; YAZICI, MERVEPublication Metadata only Detailed Immonological Analysıis of Recombinant Crimean Congo Hemorrhagic Fever Virus Nucleoprotein(2019-04-28) KARAASLAN, ELİF; ÇETİN, NESİBE SELMA; Hasanoğlu, Sevde; KILIÇ, ALİ OSMAN; KALKAN, AHMET; ÖZDARENDELİ, AYKUT; DOYMAZ, MEHMET ZİYA; KARAASLAN, ELİF; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYAPublication Open Access Cross-Reactive anti-Nucleocapsid Protein Immunity against Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus in Multiple Species.(2021-01-13T00:00:00Z) Kalkan-Yazıcı, Merve; Karaaslan, Elif; Çetin, Nesibe Selma; Hasanoğlu, Sevde; Güney, Filiz; Zeybek, Ümit; Doymaz, Mehmet Ziya; YAZICI, MERVE; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYAThe World Health Organization estimates that there may be three billion people at risk of infection by Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a highly lethal, emerging orthonairovirus carried by ticks. On the other hand, the closely related Hazara virus (HAZV), a member of the same serogroup, has not been reported as a pathogen for humans. Given the structural and phylogenetic similarities between these two viruses, we evaluated the immunological similarities of the nucleocapsid protein (NP) of these two viruses in multiple species. Strong antigenic similarities were demonstrated in anti-NP humoral immune responses against HAZV and CCHFV in multiple species using convalescent human CCHF sera, rabbit and mouse polyclonal antiserum raised against CCHFV, and mouse polyclonal antiserum against CCHFV-NP in enzyme immunoassays. We also report a convincing cross-reactivity between NPs in Western blots using HAZV-infected cell lysate as antigen and inactivated CCHFV and CCHFV-NP-immunized mice sera. These results suggest that NPs of HAZV and CCHFV share significant similarities in humoral responses across species and underline the potential utility of HAZV as a surrogate model for CCHFV.IMPORTANCE CCHFV and HAZV, members of the Nairoviridae family, are transmitted to mammals by tick bites. CCHFV is considered to be a severe threat to public health and causes hemorrhagic diseases with a high mortality rate, and there are neither preventative nor therapeutic medications against CCHFV disease. HAZV, on the other hand, is not a pathogen to humans and can be studied under BSL-2 conditions. The antigenic relationship between these viruses is of interest for vaccines and for preventative investigations. Here, we demonstrate cross-reactivity in anti-NP humoral immune response between NPs of HAZV and CCHFV in multiple species. These results underline the utility of HAZV as a surrogate model to study CCHFV infection.Publication Metadata only STABLE EXPRESSION OF PROTEINS OF CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS (CCHFV) IN EUKARYOTIC CELLS: A RELIABLE MODEL FOR INVESTIGATING VIRUS BIOLOGY AND PATHOGENESIS(2023-09-19) Bakangil Ö.; Yazıcı M.; Çetin N. S.; Doymaz M. Z.; BAKANGİL, ÖZLEM; YAZICI, MERVE; ÇETİN, NESİBE SELMA; DOYMAZ, MEHMET ZIYA