Person: ŞİŞMAN, BEHİCE HANDE
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Publication Metadata only C6 Glioma Hücre Kültüründe Timokinonun Etkileri(2017-09-23T00:00:00Z) ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZ; ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZPublication Metadata only C6 Glioma Hücre Kültüründe Timokinon’un Etkinliğinin Araştırılması(2017-04-02T00:00:00Z) ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SEYİTHANOĞLU, MEHMET HAKAN; HATİBOĞLU, MUSTAFA AZİZ; ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; SEYİTHANOĞLU, MEHMET HAKAN; HATİBOĞLU, MUSTAFA AZİZPublication Metadata only Thymoquinone is an effective treatment through induction of apoptosis in U87 human glioma cells(2017-08-25T00:00:00Z) ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; NALLI, ARİFE; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZ; ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZPublication Metadata only Evaluation of Oxidative Stress and Inflammatory Biomarkers Pre and Post-Treatment in New Diagnosed Atherosclerotic Patients(2022-02-01T00:00:00Z) Belce, Ahmet; Ozkan, Beyza Nur; Dumlu, Fatma Sena; ŞİŞMAN, BEHİCE HANDE; Guler, Eray Metin; ŞİŞMAN, BEHİCE HANDEAtherosclerosis is a chronic vascular inflammatory disease associated to oxidative stress and endothelial dysfunction. It is characterized by lipid accumulation in the arterial wall, increased hyperlipidemia, oxidative stress, lipid peroxidation, and protein oxidation. Our study included 45 patients ages of 40-60 and 45 healthy volunteers with similar demographic characteristics without any chronic disease as well. Fasting plasma glucose, BUN, creatinine, LDL-cholesterol, HDL-cholesterol, triglyceride, total cholesterol, HbA1c, and C-reactive protein (CRP) levels were measured using commercial kits by autoanalyzer. The oxidative stress biomarkers total oxidant status (TOS), total antioxidant status (TAS), total thiol (TT), native thiol (NT), catalase (CAT), paraoxonase (PON1), and arylesterase (ARES) enzyme activities were measured using photometric methods. The inflammatory biomarkers interleukin 1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), presepsin (PSPN), and raftlin (RFTN1) levels were measured with ELISA Kits. Oxidative stress index (OSI) and disulfide (DIS) were calculated. The clinical, biochemical biomarkers such as BUN, creatinine, HDL, LDL, total cholesterol, triglyceride, and CRP levels were found to be higher than the control group and lower post-treatment compared to the pre-treatment group (p <0.001). The oxidative stress parameters, TOS, OSI, and DIS levels were found to be higher than the control group, and the levels before the treatment were statistically significantly higher than after the treatment (p < 0.001). Antioxidant biomarkers TAS, TT, and NT levels were low in the patient group. Inflammatory biomarkers were highest before treatment and decreased with treatment. Oxidative stress and inflammation, which increased in atherosclerosis patients may guide disease prognosis and treatment strategies.Publication Metadata only Thymoquinone’s effect on C6 glioma cell culture(2017-03-14T00:00:00Z) ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZ; ŞİŞMAN, BEHİCE HANDE; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZPublication Metadata only Thymoquinone Enhances the Effectiveness of Gamma Knife in Glioma Cell Lines in vitro Through Inducing Apoptosis and Genotoxicity(2017-10-11T00:00:00Z) HATİBOĞLU, MUSTAFA AZİZ; GÜLER, ERAY METİN; AKDUR, KERİME; NALLI, ARİFE; ŞİŞMAN, BEHİCE HANDE; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZ; GÜLER, ERAY METİN; ŞİŞMAN, BEHİCE HANDE; KOÇYİĞİT, ABDÜRRAHİMPublication Open Access Investigation of cellular effects of thymoquinone on glioma cell(2021-01-01T00:00:00Z) Guler, Eray Metin; ŞİŞMAN, Behice Hande; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZ; ŞİŞMAN, BEHİCE HANDE; KOÇYİĞİT, ABDÜRRAHİM; HATİBOĞLU, MUSTAFA AZİZGlioblastoma, as an invasive tumor, is one of the most common primary malignant brain tumors. Despite maximum aggressive treatment, patients with glioblastoma have a dismal prognosis. Thymoquinone (TQ) has been found to show anti-cancer effects on different types of cancer. There are a few in vitro studies on the effect of TQ on glial tumors. However, the molecular mechanism of TQ-s anti-cancer effect has not been fully elucidated. In the present study, we aimed to investigate the genotoxic, apoptotic, and cytotoxic effects of TQ on C6 rat glioma cells. C6 glioma cells were analyzed after 24 h of exposure to different concentrations of TQ by the ATP cell viability assay for cytotoxicity, comet assay for genotoxicity, 2′,7′dichlorodihydrofluorescein diacetate (H2DCF-DA) for intracellular reactive oxygen species (iROS) generation, 3.3′dihexyloxacarbocyanine iodide (DiOC6(3)) for mitochondrial membrane potential, GSH/GSSG-Glo Assay for glutathione level and Fura-2AM for intracellular calcium levels. Apoptosis induction was studied by acridine orange/ethidium bromide double staining, flow cytometry, and western blotting analyses. Caspase-3, Caspase-9, Bax, Bcl-2, and pSTAT3 protein levels were determined by the western blotting method. Cytotoxicity was enhanced by TQ in C6 glioma cells in a concentration-dependent manner. TQ also induced DNA damage, apoptosis, and increased iROS. Also, MMP and GSH levels were decreased by TQ. It inhibited pSTAT3, resulting in apoptosis induction through the regulation of anti-apoptotic and pro-apoptotic proteins. Our results suggest that TQ would be an effective treatment in glioma. Further studies should support these findings.