Person: KOÇYİĞİT, ABDÜRRAHİM
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Publication Metadata only Beta-globin gene mutations in children with beta-thalassemia major from Sanliurfa province, Turkey(2011-12-01T00:00:00Z) Aycicek, Ali; Koc, Ahmet; Ozdemir, Zeynep Canan; Bilinc, Hasan; Kocyigit, ABDÜRRAHİM; Dilmec, Fuat; KOÇYİĞİT, ABDÜRRAHİMObjective: The prevalence of (beta-thalassemia in Sanhurfa province, Turkey is reported to be 2.6%-3.7%, whereas nation-wide the frequency of beta-thalassemia is 2%. This study aimed to identify the most frequent beta-thalassemia mutations in Sanhurfa province.Publication Open Access Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis(2018-01-01) Koyuncu, Ismail; Kocyigit, ABDÜRRAHİM; Ozer, Ahmet; SELEK, Sahabettin; Kirmit, Adnan; Karsen, Hasan; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİNSerologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humansPublication Metadata only Parental anxiety and affecting factors in acute paediatric blunt head injury(2014-01-01T00:00:00Z) Serinken, Mustafa; Kocyigit, ABDÜRRAHİM; Karcioglu, O.; Sengül, C.; Hatipoǧlu, C.; Elicabuk, H.; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Effectiveness of Different Surgical Flap Delay Methods and Their Systemic Toxicities.(2021-01-14T00:00:00Z) Yildiz, Kemalettin; Mirapoglu, Semih Lutfi; Kilic, Ulkan; Guneren, Ethem; Kocyigit, ABDÜRRAHİM; Kirazoglu, Ahmet; Sagir, Haci Omer; Yesiloglu, Nebil; Canter, Halil Ibrahim; Cay, Ali; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Cytotoxic, genotoxic and apoptotic activities of carvacrol from Thymus vulgaris on the human adenocarcinoma gastric cells(2015-08-01) GUNES-BAYIR, Ayse; Kiziltan, HURİYE ŞENAY; Guler, ERAY METİN; KARAKAS, Ersin; Kocyigit, ABDÜRRAHİM; GÜNEŞ BAYIR, AYŞE; KIZILTAN, HURİYE ŞENAY; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only DNA damage in children exposed to secondhand cigarette smoke and its association with oxidative stress.(2012-12-01T00:00:00Z) SHERMATOV, K; ZEYREK, D; YILDIRIM, F; KILIC, M; CEBI, N; Kocyigit, ABDÜRRAHİM; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Impact of contrast enhanced MRI on lymphocyte DNA damage and serum visfatin level(2011-08-01T00:00:00Z) Yildiz, Sema; Cece, Hasan; Kaya, Ihsan; Celik, Hakim; Taskin, Abdullah; Aksoy, Nurten; Kocyigit, ABDÜRRAHİM; Eren, Mehmet A.; KOÇYİĞİT, ABDÜRRAHİMObjectives: Nephrogenic systemic fibrosis is a novel clinical entity encountered in subjects undergoing contrast enhanced magnetic resonance imaging (MRI). The aim of the present study is to evaluate the impact of contrast enhanced MRI exposure on lymphocyte DNA damage and serum levels of visfatin.Publication Metadata only Oxidative status and lymphocyte DNA damage in patients with acute pancreatitis and its relationship with severity of acute pancreatitis(2016-01-01) DUR, Ali; KOCAMAN, Orhan; Kocyigit, ABDÜRRAHİM; Turkdogan, Kenan Ahmet; Sonmez, ERTAN; KESKIN, Siddika; YIGIT, Mehmet; Gulen, BEDİA; KILIC, Elif; Uysal, Omer; KOÇYİĞİT, ABDÜRRAHİM; SÖNMEZ, ERTAN; GÜLEN, BEDİA; UYSAL, ÖMERBackground/Aims: Acute pancreatitis (AP) is a life-threatening disease with a rising incidence. The aim of this study was to investigate the association between oxidative status, lymphocyte deoxyribonucleic acid (DNA) damage, and acute pancreatitis.Publication Metadata only Comparison of the efficacy of multiple antioxidant and hyperbaric oxygen treatments in the prevention of ischemia and necrosis of local random McFarlane skin flap.(2021-02-26T00:00:00Z) Dogan, Remzi; Metin Guler, Eray; Kocyigit, ABDÜRRAHİM; Bayindir, Nihan; Mirasoglu, Bengisu Oroglu; Yenigun, Alper; DOĞAN, REMZI; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDES; YENİGÜN, ALPER; ÖZTURAN, ORHANPublication Metadata only Sperm DNA damage and seminal oxidative status after shock-wave lithotripsy for distal ureteral stones(2011-11-01T00:00:00Z) Gulum, Mehmet; Yeni, Ercan; Kocyigit, ABDÜRRAHİM; Taskin, Abdullah; Savas, Murat; Ciftci, Halil; Altunkol, Adem; KOÇYİĞİT, ABDÜRRAHİMObjective: To investigate the relationship between level of sperm DNA damage, seminal oxidative status, and shock-wave lithotripsy (SWL) for distal ureteral stones.