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KOÇYİĞİT, ABDÜRRAHİM

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Author: Kocyigit, ABDÜRRAHİM × Has files: true ×

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    PublicationOpen Access
    Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis
    (2018-01-01) Koyuncu, Ismail; Kocyigit, ABDÜRRAHİM; Ozer, Ahmet; SELEK, Sahabettin; Kirmit, Adnan; Karsen, Hasan; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİN
    Serologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humans
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    PublicationOpen Access
    Dexpanthenol and ascorbic acid ameliorate colistin-induced nephrotoxicity in rats.
    (2021-01-01T00:00:00Z) Aslan, T; Guler, E M; Dundar, T; Cakir, A; Gulgec, A S; Huseyinbas, O; Celikten, M; Coban, G; Hakyemez, I N; Kocyigit, ABDÜRRAHİM; Durdu, B; DÜNDAR, TOLGA TURAN; KOÇYİĞİT, ABDÜRRAHİM
    Objective: Colistin is a potent antibiotic which is mainly preferred in the treatment of multidrug-resistant (MDR) gram-negative bacilli. However, due to the increased risk of acute kidney injury following its use, the clinical application is limited. This nephrotoxicity is known to be induced by oxidative stress and related inflammation. In this study on rats, potent antioxidants Dexpanthenol (DEX) and Ascorbic acid (Vit C) have been administered in combination with Colistin to find out whether they would weaken Colistin's nephrotoxic effects. Materials and methods: Inflammation biomarkers were studied with enzyme-linked immunosorbent assay (ELISA) kits, and oxidative stress biomarkers were studied with different photometric methods in blood and tissue samples taken after treatment with DEX and Vit C in rats with colistin nephrotoxicity. In addition, inflammation and necrosis in the kidney tissues were examined pathologically. Results: It has been observed in the serum and tissue samples that DEX and Vit C decrease oxidative stress and inflammation biomarkers, therefore acting as nephroprotective agents. Conclusions: These compounds have been found to ameliorate the nephrotoxic effects of Colistin, which were demonstrated in the rats treated with Colistin, as well as the combinations.
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    PublicationOpen Access
    Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma
    (2015-01-01) DILEK, Fatih; Ozkaya, EMİN; Kocyigit, ABDÜRRAHİM; Yazici, MEBRURE; KESGIN, Siddika; GEDIK, Ahmet Hakan; Cakir, ERKAN; ÖZKAYA, EMİN; KOÇYİĞİT, ABDÜRRAHİM; YAZICI, MEBRURE; ÇAKIR, ERKAN
    Background: There is ample knowledge reported in the literature about the role of oxidative stress in asthma pathogenesis. It is also known that the interaction of reactive oxygen species with DNA may result in DNA strand breaks. The aim of this study was to investigate if montelukast monotherapy affects oxidative stress and DNA damage parameters in a population of pediatric asthma patients. Methods: Group I consisted of 31 newly diagnosed asthmatic patients not taking any medication, and group II consisted of 32 patients who had been treated with montelukast for at least 6 months. Forty healthy control subjects were also enrolled in the study. Plasma total oxidant status (TOS) and total antioxidant status (TAS) were measured to assess oxidative stress. DNA damage was assessed by means of alkaline comet assay. Results: The patients in both group I and group II had statistically significant higher plasma TOS (13.1 ± 4 and 11.1 ± 4.1 μmol H2O2 equivalent/liter, respectively) and low TAS levels (1.4 ± 0.5 and 1.5 ± 0.5 mmol Trolox equivalent/liter, respectively) compared with the control group (TOS: 6.3 ± 3.5 μmol H2O2 equivalent/liter and TAS: 2.7 ± 0.6 mmol Trolox equivalent/liter; p < 0.05). DNA damage was 18.2 ± 1.0 arbitrary units (a.u.) in group I, 16.7 ± 8.2 a.u. in group II and 13.7 ± 3.4 a.u. in the control group. There were statistically significant differences only between group I and the control group (p < 0.05). Conclusions: According to the findings, montelukast therapy makes only minimal but not statistically significant improvement in all TOS, TAS and DNA damage parameters.
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    PublicationOpen Access
    Antioxidant and toxicological in-vivo and in-vitro examination of licorice extract
    (2016-09-01) Bektay, MUHAMMED YUNUS; Uckaya, FATİH; Guler, E. M.; Bayindir, NİHAN; Kocyigit, ABDÜRRAHİM; Esrefoglu, MUKADDES; Topcu, GÜLAÇTI; BEKTAY, MUHAMMED YUNUS; UÇKAYA, FATİH; GÜLER, ERAY METİN; BAYINDIR, NİHAN; KOÇYİĞİT, ABDÜRRAHİM; EŞREFOĞLU, MUKADDES; TOPÇU, GÜLAÇTI
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    PublicationOpen Access
    Oxidative Stress in Children with Chronic Spontaneous Urticaria
    (2016-01-01) DILEK, Fatih; Ozceker, Deniz; Ozkaya, EMİN; Guler, Nermin; Tamay, Zeynep; KESGIN, Siddika; Yazici, MEBRURE; Kocyigit, ABDÜRRAHİM; ÖZKAYA, EMİN; YAZICI, MEBRURE; KOÇYİĞİT, ABDÜRRAHİM
    The pathogenesis of chronic spontaneous urticaria (CSU) has not been fully understood; nevertheless, significant progress has been achieved in recent years. The aim of this study was to investigate the possible role of reactive oxygen species (ROS) in the pathogenesis of CSU. Sixty-two children with CSU and 41 healthy control subjects were enrolled in the study. An extensive evaluation of demographic and clinical features was done, and serum oxidative stress was evaluated by plasma total oxidant status (TOS) and total antioxidant status (TAS) measurements.The median value of plasma TOS was found to be 10.49 𝜇mol H2O2 equiv./L (interquartile range, 7.29–17.65) in CSU patients and 7.68 𝜇mol H2O2 equiv./L (5.95–10.39) in the control group. The difference between the groups was statistically significant (𝑝 = 0.003). Likewise, the median plasma TAS level in the CSU group was decreased significantly compared to that of the control group (2.64 [2.30–2.74] versus 2.76 [2.65–2.86] mmol Trolox equiv./L, resp., 𝑝 = 0,001). Our results indicated that plasma oxidative stress is increased in children with CSU when compared to healthy subjects, and plasma oxidative stress markers are positively correlated with disease activity
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    PublicationOpen Access
    Comparison of the biochemical and radiological criteria for lumbar disc degeneration
    (2018-09-01) Seyithanoglu, MEHMET HAKAN; Kitis, SERKAN; Ozer, Omer Faruk; Kocyigit, ABDÜRRAHİM; DÜNDAR, Tolga; Papaker, Meliha Gundeg; SEYİTHANOĞLU, MEHMET HAKAN; KİTİŞ, SERKAN; ÖZER, ÖMER FARUK; KOÇYİĞİT, ABDÜRRAHİM
    Background: The relationship between radiological degeneration criteria on lumbar magnetic resonance imaging (MRI) and both the keratan sulfate (KS) and chondroitin sulfate (ChS) levels was examined in disc material taken from patients undergoing lumbar disc herniation (LDH) surgery. To examine whether the biochemical and radiological degeneration criteria testing the reliability of radiological degeneration findings agreed and to evaluate the contribution of the KS/ChS ratio to disc form (protruding or extruding).
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    PublicationOpen Access
    Does intra-articular fracture change the lubricant content of synovial fluid?
    (2015-06-03) CEYLAN, Hasan H.; Erdil, Mehmet; Polat, Gokhan; Kara, DENİZ; KILIC, Elif; Kocyigit, ABDÜRRAHİM; Tuncay, Ibrahim; KARA, DENİZ; KOÇYİĞİT, ABDÜRRAHİM; TUNCAY, İBRAHİM
    Background: Lubrication function is impaired and the lubricant content of synovial fluid (SF) changes immediately after plateau tibia fractures. Here, we aimed to analyze the lubricant content of SF at chronic term following plateau tibia fracture. Methods: Forty-eight surgically treated patients without joint incongruency (<2 mm displacement) were included in the study. Joint aspiration had been possible in 16 of the participants. However, sampling could be made from healthy knees in only ten of these patients. Twenty-six SF samples (16 injured knees, 10 healthy knees) were analyzed for concentrations of hyaluronic acid (HA), proteoglycan-4 (PRG4), TNF-α, IL-1β, and IL-6. Results: The group of experimental samples were obtained at a mean of 31 (12–66) months after injury from patients with a mean age of 45.1 (32–57) years. There were no relationships detected between biochemical analysis results and patient ages, sexes, postoperative time, and fracture type. After excluding six patients for whom we could not sample from their healthy knee, ten patients’ values were compared with paired Wilcoxon signed rank test and no significant differences detected between the healthy and injured knee in terms of the SF concentrations of HA and PRG4 (p = 0.225 and 0.893, respectively). Similarly, there were no statistically significant differences in SF sample concentrations of TNF-α, IL-1β, and IL-6 between healthy and injured knees. Conclusions: Despite acute changes, the long-term concentrations of HA and PRG4 were similar after plateau tibial fracture. We could not detect any concentration level differences between healthy knees and injured knees regarding HA and PRG4 in the long-term follow-up.
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    PublicationOpen Access
    DNA damage and oxidative status in PFAPA syndrome
    (2015-10-01) TUGRUL, Selahattin; Dogan, REMZİ; Kocyigit, ABDÜRRAHİM; Torun, EMEL; SENTURK, EROL; Ozturan, ORHAN; TUĞRUL, SELAHATTİN; DOĞAN, REMZI; KOÇYİĞİT, ABDÜRRAHİM; TORUN, EMEL; ŞENTÜRK, EROL; ÖZTURAN, ORHAN
    Objective: PFAPA syndrome is a clinical entity of unknown etiology which presents with periodic episodes of fever, aphthous stomatitis, tonsillitis or pharyngitis, and cervical adenitis. In this study we investigated DNA damage and the oxidative stress parameters in patients diagnosed with PFAPA, to elucidate the underlying pathophysiological mechanism of this syndrome. Methods: Thirty-one patients diagnosed with PFAPA (Group 1), 22 patients diagnosed with normal tonsillitis or pharyngitis (Group 2), and 20 healthy volunteers (Group 3) were included in our study. Heparinized peripheral blood samples were drawn from all patients and volunteers. DNA damage was assessed by single cell alkaline electrophoresis assay in peripheral mononuclear leukocytes. Plasma levels of total antioxidant status (TAS) and total oxidative status (TOS) were determined by using a novel automated measurement method, and oxidative stress index (OSI) was calculated. Results: DNA damage in the mononuclear leukocytes of Group 1 was significantly higher than that of Group 2 and Group 3. The oxidative stress parameters revealed that the TOS and OSI values of Group 1 were significantly higher than those of Group 2 and Group 3. TAS values of Group 1 were significantly lower than those of Group 2 and Group 3. Correlation analysis of Group 1 demonstrated a significant correlation between TOS, one of the oxidative stress parameters, and DNA damage. Correlations between DNA damage and C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) values were also significant. Conclusion: Our study indicated that both the inflammatory and the oxidative stress parameters were significantly increased in patients with PFAPA syndrome, accompanied by a significant positive correlation between DNA damage and oxidative stress.
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    PublicationOpen Access
    Plasma total thiol pool in children with asthma: Modulation during montelukast monotherapy
    (2016-03-01) DILEK, Fatih; Ozkaya, EMİN; Kocyigit, ABDÜRRAHİM; Yazici, MEBRURE; Guler, ERAY METİN; DUNDAROZ, Mehmet Rusen; ÖZKAYA, EMİN; KOÇYİĞİT, ABDÜRRAHİM; YAZICI, MEBRURE; GÜLER, ERAY METİN
    Background: Inflammation, which is a hallmark of asthma, is one of the main sources of oxidative stress in the human body. Thiols are powerful antioxidants that protect cells against the consequences of oxidative stress. We aimed to investigate whether asthma and montelukast monotherapy affect the total plasma thiol pool in children. Methods: A total of 60 children with asthma and 35 healthy controls participated in the study. Group I consisted of newly diagnosed asthmatics who did not have regular anti-asthmatic therapy previously. Group II consisted of patients who had been undertaking montelukast monotherapy regularly for at least 4 months. Plasma total antioxidant status (TAS) and plasma total thiol (PTT) were measured using spectrophotometric methods. Results: Bronchial asthma patients in both groups I and II had decreased median TAS levels compared with the control group (1.59 [interquartile range, 1.04–1.70] and 1.67 [1.50–1.75] vs. 2.98 [2.76–3.16] Trolox equiv./L, respectively; P <0.001). Group I had decreased PTT concentrations compared with the control group (0.18 [0.16–0.20] vs. 0.21 [0.19–0.22] mmol/L; P <0.001), and group II had similar PTT levels to the control group (0.20 [0.17–0.22] mmol/L; P >0.05). In addition, the median TAS and PTT levels for groups I and II were not statistically different (P >0.05). There was a positive correlation between TAS and PTT levels (rho = 0.38, P <0.05) in group I. Conclusion: In order to balance the oxidative stress, both TAS and PTT which are markers of the antioxidant system are reduced in children with asthma. Montelukast monotherapy can limit oxidative stress and thus restore PTT levels but not TAS levels in asthmatic children.
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    PublicationOpen Access
    Oxidative Stress Status in Childhood Obesity: A Potential Risk Predictor
    (2016-10-13) Kılıç, Elif; Ozer, Omer Faruk; Erek, Aybala Toprak; ERMAN, HAYRİYE; Torun, EMEL; AYHAN, Siddika Kesgin; Caglar, HİFA GÜLRU; Selek, Sahbettin; Kocyigit, ABDÜRRAHİM; ÖZER, ÖMER FARUK; TORUN, EMEL; ÇAĞLAR, HİFA GÜLRU; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİM
    Background: Childhood obesity characterized by excessive fat in the body is one of the most serious health problems worldwide due to the social, medical, and physiological complications. Obesity and associated diseases are triggering factors for oxidative stress and inflammation. The aim of this study was to explore the possible association between childhood obesity and inflammatory and oxidative status. Material/Methods: Thirty-seven obese children and 37 healthy controls selected from among children admitted to BLIND University Paediatrics Department were included in the study. Anthropometric measurements were performed using standard methods. Glucose, lipid parameters, CRP, insulin, total oxidant status (TOS), total anti-oxidant status (TAS) levels, and total thiol levels (TTL) were measured in serum. HOMA index (HOMA-IR) were calculated. The differences between the groups were evaluated statistically using the Mann-Whitney U test. Results: Body mass index was significantly higher in the obese group (median: 28.31(p<0.001). Glucose metabolism, insulin, and HOMA-IR levels were significantly higher in the obese group (both p<0.001). Total cholesterol, HDL cholesterol, LDL cholesterol, and triglyceride levels were significantly higher in the obese group (p<0.001). TAS (med: 2.5 µmol Trolox eq/L (1.7–3.3)) and TOS (med: 49.1 µmol H2 O2 eq/L (34.5–78.8)) levels and TTL (med: 0.22 mmol/L (0.16–0.26)) were significantly higher in the obese group (p=0.001). CRP levels showed positive correlation with TOS and negative correlation with TTL levels (p=0.005, r=0.473; p=0.01, r=–0.417; respectively). TTL levels exhibited negative correlation with TOS levels (p=0.03, r=–0.347). Conclusions: In conclusion, obese children were exposed to more oxidative burden than children with normal weight. Increased systemic oxidative stress induced by childhood obesity can cause development of obesity-related complications and diseases. Widely focussed studies are required on the use of oxidative parameters as early prognostic parameters in detection of obesity-related complications