Person: KOÇYİĞİT, ABDÜRRAHİM
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Publication Metadata only Beta-globin gene mutations in children with beta-thalassemia major from Sanliurfa province, Turkey(2011-12-01T00:00:00Z) Aycicek, Ali; Koc, Ahmet; Ozdemir, Zeynep Canan; Bilinc, Hasan; Kocyigit, ABDÜRRAHİM; Dilmec, Fuat; KOÇYİĞİT, ABDÜRRAHİMObjective: The prevalence of (beta-thalassemia in Sanhurfa province, Turkey is reported to be 2.6%-3.7%, whereas nation-wide the frequency of beta-thalassemia is 2%. This study aimed to identify the most frequent beta-thalassemia mutations in Sanhurfa province.Publication Open Access Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis(2018-01-01) Koyuncu, Ismail; Kocyigit, ABDÜRRAHİM; Ozer, Ahmet; SELEK, Sahabettin; Kirmit, Adnan; Karsen, Hasan; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİNSerologic tests for brucellosis aim to detect antibodies produced against membranous lipopolysaccharide of bacteria. Diagnostic use of this method is limited due to false positiveness. This study evaluates an alternative antigen to lipopolysaccharides (LPS), outer membrane 28-precursor-protein, of Brucella melitensis Rev1 for its diagnostic value. Omp28 precursor of B. melitensis Rev1 was cloned, expressed, and purified. 6-His and sumo epitope tags were used to tag the protein at N-termini. Omp28 gene was amplified based on the ORF sequence and cloned into a pETSUMO vector. The recombinant construct was propagated in Escherichia coli One Shot® Mach1™ cells then transformed into E. coli BL21(D3) cells for protein expression. The purified protein was studied in an indirect ELISA for diagnosis of brucellosis. Sera samples from 60 patients were screened by ELISA and the results were compared to Rose Bengal plate test. Recombinant antigen-based iELISA has given a successful outcome with the sensitivity, specificity, positive predictive value, and negative predictive value of 87.8%, 96.2%, 96.6%, and 78.78%, respectively. In conclusion, recombinant production and purification of the immunodominant Omp28 precursor protein has been achieved successfully in a one-step process with efficient yield and can be used for diagnosis of brucellosis in humansPublication Metadata only Parental anxiety and affecting factors in acute paediatric blunt head injury(2014-01-01T00:00:00Z) Serinken, Mustafa; Kocyigit, ABDÜRRAHİM; Karcioglu, O.; Sengül, C.; Hatipoǧlu, C.; Elicabuk, H.; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Cytotoxic, genotoxic and apoptotic activities of carvacrol from Thymus vulgaris on the human adenocarcinoma gastric cells(2015-08-01) GUNES-BAYIR, Ayse; Kiziltan, HURİYE ŞENAY; Guler, ERAY METİN; KARAKAS, Ersin; Kocyigit, ABDÜRRAHİM; GÜNEŞ BAYIR, AYŞE; KIZILTAN, HURİYE ŞENAY; GÜLER, ERAY METİN; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only DNA damage in children exposed to secondhand cigarette smoke and its association with oxidative stress.(2012-12-01T00:00:00Z) SHERMATOV, K; ZEYREK, D; YILDIRIM, F; KILIC, M; CEBI, N; Kocyigit, ABDÜRRAHİM; KOÇYİĞİT, ABDÜRRAHİMPublication Metadata only Impact of contrast enhanced MRI on lymphocyte DNA damage and serum visfatin level(2011-08-01T00:00:00Z) Yildiz, Sema; Cece, Hasan; Kaya, Ihsan; Celik, Hakim; Taskin, Abdullah; Aksoy, Nurten; Kocyigit, ABDÜRRAHİM; Eren, Mehmet A.; KOÇYİĞİT, ABDÜRRAHİMObjectives: Nephrogenic systemic fibrosis is a novel clinical entity encountered in subjects undergoing contrast enhanced magnetic resonance imaging (MRI). The aim of the present study is to evaluate the impact of contrast enhanced MRI exposure on lymphocyte DNA damage and serum levels of visfatin.Publication Metadata only Oxidative status and lymphocyte DNA damage in patients with acute pancreatitis and its relationship with severity of acute pancreatitis(2016-01-01) DUR, Ali; KOCAMAN, Orhan; Kocyigit, ABDÜRRAHİM; Turkdogan, Kenan Ahmet; Sonmez, ERTAN; KESKIN, Siddika; YIGIT, Mehmet; Gulen, BEDİA; KILIC, Elif; Uysal, Omer; KOÇYİĞİT, ABDÜRRAHİM; SÖNMEZ, ERTAN; GÜLEN, BEDİA; UYSAL, ÖMERBackground/Aims: Acute pancreatitis (AP) is a life-threatening disease with a rising incidence. The aim of this study was to investigate the association between oxidative status, lymphocyte deoxyribonucleic acid (DNA) damage, and acute pancreatitis.Publication Metadata only Sperm DNA damage and seminal oxidative status after shock-wave lithotripsy for distal ureteral stones(2011-11-01T00:00:00Z) Gulum, Mehmet; Yeni, Ercan; Kocyigit, ABDÜRRAHİM; Taskin, Abdullah; Savas, Murat; Ciftci, Halil; Altunkol, Adem; KOÇYİĞİT, ABDÜRRAHİMObjective: To investigate the relationship between level of sperm DNA damage, seminal oxidative status, and shock-wave lithotripsy (SWL) for distal ureteral stones.Publication Metadata only Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters(2012-09-01T00:00:00Z) Erturk, Cemil; Altay, Mehmet Akif; Selek, Sahbettin; Kocyigit, ABDÜRRAHİM; SELEK, ŞAHABETTİN; KOÇYİĞİT, ABDÜRRAHİMBackground. The aim of this study was to investigate serum paraoxonase-1 (PON1) activity and oxidative/anti-oxidative status in knee osteoarthritis (OA), and evaluate their relationship using radiological and clinical parameters. Materials and methods. The study population comprised 127 patients with knee OA and 107 healthy volunteers. Patients with knee OA were divided into four subgroups according to the Kellgren-Lawrence (K&L) grading scale. In addition, each patient was clinically evaluated by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Serum PON1 activity was measured spectrophotometrically. Oxidative status was assessed by measuring serum lipid hydroperoxide (LOOH) and total oxidant status (TOS). Anti-oxidative status was assessed by measuring serum free sulfydryl groups (-SH = total thiol) and total antioxidant capacity (TAC). Oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods. Results. Serum PON1 activity was significantly lower in the knee OA group compared to the control group (p 0.05). The lowest and highest mean serum PON1 activities were detected in patients with grades 4 and 1, respectively (ANOVA p < 0.001). In multiple regression analysis, WOMAC score was independently associated with serum PON1 activity (beta = -0.248, p = 0.027). Conclusions. Decreased serum PON1 activity and elevated LOOH, TOS, and OSI levels may be associated with knee OA, and serum PON1 activity may be a useful adjunctive indicator of the severity of knee OA for follow-up.Publication Metadata only Mononuclear leukocyte DNA damage and oxidative stress: The association with smoking of hand-rolled and filter-cigarettes(2011-04-03T00:00:00Z) Kocyigit, ABDÜRRAHİM; Selek, ŞAHBETTİN; Celik, Hakim; Dikilitas, Murat; KOÇYİĞİT, ABDÜRRAHİM; SELEK, ŞAHABETTİNCigarette smoking is a major cause of human cancer at various sites, although its carcinogenic mechanisms still remain unestablished. Based on the use of a filter, cigarette smoke can be divided into a gas phase and a tar phase. Both contain different concentrations of oxidants, free radicals and tobacco-specific carcinogens. To explore the effects of both filtered and non-filtered cigarette smoke on DNA damage and oxidative status, we measured the level of mononuclear leukocyte DNA damage by use of the single-cell gel electrophoresis (Comet) assay. We also determined malondialdehyde (MDA), protein carbonyl content (PC) and total antioxidative capacity (TAC) levels in blood plasma of smokers of manufactured filter-cigarettes and of hand-rolled cigarettes. Cotinine levels were also measured in plasma to estimate the degree of smoking. Mononuclear leukocyte DNA damage, plasma MDA, plasma PC and plasma cotinine levels were found significantly higher, while plasma TAC levels were found significantly lower in smokers of filter-cigarettes and smokers of hand-rolled cigarettes, compared with control subjects. TAC levels in hand-rolled and manufactured filter-cigarette smokers were not significantly different from each other. However, the levels of DNA damage. plasma MDA, plasma cotinine, and plasma protein oxidation were significantly higher in hand-rolled cigarette smokers than in filter-cigarette smokers. There was a significant positive correlation between MDA and DNA damage in both hand-rolled cigarette smokers and manufactured filter-cigarette smokers. This study indicates that smoking of hand-rolled cigarettes has stronger genotoxic and oxidative effects on the metabolism than smoking of manufactured filter-cigarettes. We propose that these harmful effects could be attributed to the higher level of oxidants. (C) 2011 Elsevier B.V. All rights reserved.