Person: KÖSE, HAYRULLAH
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Publication Analysis of Spinal Nerve Conduction Measurement With Epidural Stimulation in Rats(2010-01-01T00:00:00Z) Kose, Hayrullah; Basoglu, HARUN; KÖSE, HAYRULLAH; BAŞOĞLU, HARUNNerve conduction velocity (NCV) is a very common parameter to diagnose neuromuscular and neurodegenerative diseases. Animal model of the diseases is most suitable model for new treatments. However measuring of NCV in rats brings some difficulties such as short extremities, thickness of skin and small length of peripheral nerves. Our purpose is to overcome these difficulties by measuring spinal NCV on lumbar level with epidural stimulation. In this study Wistar-Albino strain of rats were used. Under general anesthesia, rats were electrically stimulated with monopalar needle electrodes from two different points in spinal cord; L1-L2 and L5-L6 spinal segment and EMG was recorded from surface of the gastrocnemius muscle. In addition, NCV was also measured from sciatic nerve of the rats. The mean of calculated spinal nerve conduction velocity of male rats was 72.4 m/s +/- 3.77 and of female was 68.24 m/s +/- 5.97 from compound muscle action potential (CMAP) analysis. As a result, spinal nerve conduction velocity can be measured with the usage of EMG that produced with the epidural stimulation of spinal cord. Furthermore it is more relevant to induce EMG with epidural spinal stimuli than skin surface stimuli of spine in rats.Publication Protective Effects of Leflunomide on Intestinal Ischemia-Reperfusion Injury Leflunomide against intestinal ischemia-reperfusion(2010-02-01T00:00:00Z) Yildiz, Yuksel; Kose, HAYRULLAH; Cecen, Serpil; Ergin, Kemal; Demir, Ece Mine; Serter, Mukadder; KÖSE, HAYRULLAHAim The aim of this study was to investigate the possible protective effects of leflunomide, which has antioxidant and anti-inflammatory properties, against intestinal IR injury in rats. Materials and Methods Forty female Wistar albino rats were divided into six groups: control (n = 5), drug control (n = 7), sham operated (n = 7), IR alone (n = 7), IR plus vehicle ( IR + vehicle, n = 7) and IR plus 20 mg/kg leflunomide (IR + Leflunomide, n = 7). While rats were pretreated intragastrically with leflunomide (20 mg/kg) and vehicle in three doses prior to the experiment, respectively, in the IR + Leflunomide and IR + vehicle groups, no additional application was done in the IR alone group. Intestines were exteriorized, and the superior mesenteric artery was occluded for 45 min ischemia, and then the clamp was removed for 120 min reperfusion. After the experiment, the intestines were removed for biochemical and histological examinations. Additionally, blood samples were taken for measurements of antioxidant parameters. Results The intestinal IR significantly increased the MDA level and MPO activity; however, treatment with leflunomide reversed those findings (P < 0.05). The CAT activity of the IR + Leflunomide group was significantly higher than in the IR groups (P < 0.05). The SOD activity was increased in the intestinal IR group, and leflunomide treatment reversed that, too (P < 0.05). The light microscopic findings showed that IR caused mucosal necrosis and leflunomide treatment reduced the morphological alterations associated with IR (P < 0.05). Conclusion Intestinal IR injury may be reversed by the anti-inflammatory and antioxidant actions of leflunomide.Publication Apoptosis rate in malignant mammary tumours in bitches(2011-03-01T00:00:00Z) Seyrek, K.; Seyrek-Intas, K.; ÖZALP, RABİA GÖZDE; Yilmazbas, G.; ÖZYİĞİT, MUSA ÖZGÜR; Kose, HAYRULLAH; Aksit, H.; Bildik, A.; Sandikci, M.; KÖSE, HAYRULLAHAs the equilibrium between cell division and cell death (apoptosis) determines the growth of a tumour, the aim of the study was to evaluate the apoptosis frequency in dog mammary tumours both by the TdT-mediated dUTP-biotin nick end Libelling (TUNEL) method and by the ELISA detection of nucleosoms in cell homogenates. For that, apoptosis rates were investigated in 35 malignant tumours (16 malignant mixed tumours, 12 lobular adenocarcinomas, 3 cirrhotic adenocarcinomas and 4 myoepitheliomas) and in 10 normal mammary tissues. Whereas the apoptotic cells were found abundant and homogeneously distributed throughout the healthy breast tissues by the TUNEL method, they appeared less numerous in malignant tumours and were essentially located within mammary acini in few areas. The average amounts of nucleosoms proportional to the absorbance at 405 nm in the ELISA test in cell homogenates from malignant tumours were lower than in the healthy mammary tissues but according to the tumour type, the difference with controls was significant only for the malignant mixed tumours (P < 0.05). These results show that apoptosis rate tended globally to decrease in dog malignant mammary tumours, favouring in this way the selection and expansion of apoptosis-resistant neoplastic cells.Publication Effects of Caffeic Acid Phenethyl Ester on Penicillin-induced Epileptiform Activity(2016-09-01T00:00:00Z) Kozan, Ramazan; Pala, Mukaddes; KÖSE, HAYRULLAH; Akyuz, Enes; KÖSE, HAYRULLAH; AKYÜZ, ENESPublication The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine-induced acute pancreatitis in rats(2016-01-01T00:00:00Z) Dur, A.; Kose, H.; KOÇYİĞİT, ABDÜRRAHİM; Kocaman, O.; Ismayilova, M.; Sonmez, F.C.; KÖSE, HAYRULLAH; KOÇYİĞİT, ABDÜRRAHİMPublication Cuprizone-induced demyelination in Wistar rats; electrophysiological and histological assessment(2013-10-01T00:00:00Z) Basoglu, HARUN; Boylu, N. T.; KOSE, H.; BAŞOĞLU, HARUN; KÖSE, HAYRULLAHOBJECTIVES: Multiple Sclerosis (MS) is a disease that affects the Central Nervous System by destructing myelin shield and also can affects the peripheral nervous system. Demyelination is acquired characteristics disease and appears with the degeneration of myelin which protects the axons. Cuprizone (CPZ) model is a toxic demyelination model. The purpose of this study was to develop an MS model by cuprizone exposure toWistar rats.Publication Anticonvulsant Effect of Ramelteon on the Seizures Induced by Pentylenetetrazole in Rats(2016-09-01T00:00:00Z) Pala, Mukaddes; Kozan, Ramazan; KÖSE, HAYRULLAH; KÖSE, HAYRULLAHPublication The Effects of Negative Air Ions upon Some Biochemical Parameters in Rats(2010-01-01T00:00:00Z) Kose, HAYRULLAH; Seyrek, Kamil; Kiral, Funda; KÖSE, HAYRULLAHNegative air ions (NAI) are capable of evoking a wide range of responses in bacteria, protozoa, higher plants, insects and animals, as well as in humans. However, their precise mechanism of action is uncertain. There is evidence to show that NAI are able to influence mood, behaviour, and performance of certain tasks. In this study, rats were put into two groups, a control and an experimental group. Animals in the experimental group were exposed to an atmosphere enriched with NAI for four weeks. At the end of the experiment, we determined the blood glutathione (GSH), malondialdehyde (MDA), ceruloplasmin, hemoglobin and packed cell volume (PCV) concentrations of the rats. MDA concentrations in the experimental group were lower than that of the control animals (4.02 mu mol/l and 5.11 mu mol/l, respectively). Likewise, in the experimental animals, ceruloplasmin levels were also reduced from 33.18 mg/dl to 26.27 mg/dl, whereas no alterations in hemoglobin or glutathione concentrations were detected. An increase from 43.80% to 46.10 % was shown in the PCV content of the experimental rats. In addition, the mean weight gain of the experimental group was less than that of the control group. However, in terms of these parameters, we were unable to detect any significant differences between the two groups. In conclusion, contrary to some in vitro studies, this in vivo animal study suggests that NAI has limited or no effect upon certain biochemical parameters.